Abstract: A method for operating a point laser-scanning microscope includes scanning a sample with a focused illumination laser beam; recording a plurality of images by detecting elements being configurable to an intensity mode, in which the recorded images are intensity images gi,j(n) related to photons collected during an entire dwell time of the illumination beam on an individual position n, or to a time-resolved mode, in which the recorded images are time-resolved images gi,jt(n, t), the collected photons being discriminated based on their arrival times to individual detecting elements; calculating a fingerprint image a by summing the plurality of intensity images gi,j(n) over all positions n; estimating shift matrices sx and sy from the intensity images gi,j(n); reconstructing at least one of a time-resolved object function ft and an intensity object function f; and visualizing at least one of a high-resolution time-resolved image ft˜ and a high-resolution intensity image f˜.

Abstract: A photodetector may include an array of microcells and an output module configured to collect, from each microcell, an output signal indicative of a photon detection and to combine the collected output signals in at least one output line. Each microcell comprises a first device and a second device, wherein at least one of the devices is a photosensitive device capable of detecting the photon; a time-gating module connected to said at least one photosensitive device and configured to provide a gate signal to the at least one photosensitive device to activate it; and a readout module configured to receive, upon arrival of the photon on the at least one activated photosensitive device, a corresponding signal from the at least one activated photosensitive device and, on the basis of the received signal, to provide the output signal to the output module.

Abstract: A method for operating a point laser-scanning microscope comprises scanning a sample with a focused illumination laser beam; recording a plurality of images by detecting elements being configurable to an intensity mode, in which the recorded images are intensity images gi,j(n) related to photons collected during an entire dwell time of the illumination laser beam on an individual position n, or to a time-resolved mode, in which the recorded images are time-resolved images gi,jt(n,t), the collected photons being discriminated based on their arrival times to individual detecting elements; calculating a fingerprint image a by summing the plurality of intensity images gi,j(n) over all positions n; estimating shift matrices sx and sy from the intensity images gi,j(n); reconstructing at least one of a time-resolved object function ft and an intensity object function f; and visualizing at least one of a high-resolution time-resolved image ft˜ and a high-resolution intensity image f˜.