Abstract: Disclosed herein is a low cost and rapid microfluidic based method and test device for quantifying male fertility potential. The device can simultaneously measure three critical semen parameters rapidly, namely live sperm concentration, motile sperm concentration, and sperm motility. The device includes a transparent substrate and a top sheet with two holes therethrough and an intermediate sheet sandwiched between the substrate and the top sheet. The wells formed by holes form a concentration measuring well (C) and a motility well (M) formed by the top sheet with these two holes bonded to the intermediate sheet. A colorimetric agent is located on the top surface of the intermediate sheet at the bottom of each well which changes color when in contact with sperm. In the motility well a porous membrane is located on top of the colorimetric agent and a liquid buffer may be placed on the top surface of the porous membrane.

Abstract: Disclosed herein is a low cost and rapid microfluidic based method and test device for quantifying male fertility potential. The device can simultaneously measure three critical semen parameters rapidly, namely live sperm concentration, motile sperm concentration, and sperm motility. The device includes a transparent substrate and a top sheet with two holes therethrough and an intermediate sheet sandwiched between the substrate and the top sheet. The wells formed by holes form a concentration measuring well (C) and a motility well (M) formed by the top sheet with these two holes bonded to the intermediate sheet. A colorimetric agent is located on the top surface of the intermediate sheet at the bottom of each well which changes color when in contact with sperm. In the motility well a porous membrane is located on top of the colorimetric agent and a liquid buffer may be placed on the top surface of the porous membrane.