Patents by Inventor Max Schubert
Max Schubert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11939571Abstract: Expressing guide nucleic acids (e.g., gRNA) from the same oligonucleotide that contains donor sequence permits the high efficiency, simultaneous transformation of a population of cells with both substrates. Using oligonucleotide chip array technology, one can construct thousands of oligonucleotides with customized gRNA and donor sequence in a cost effective manner. In combination, one can efficiently modify endogenous and exogenous genes.Type: GrantFiled: June 12, 2017Date of Patent: March 26, 2024Assignees: President and Fellows of Harvard College, The General Hospital CorporationInventors: Xiaoge Guo, Alejandro Chavez, Max Schubert, Eric Kelsic
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Publication number: 20220325303Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: ApplicationFiled: June 14, 2022Publication date: October 13, 2022Inventors: Andrew HORWITZ, Kristy Michelle HAWKINS, Max SCHUBERT, Wayne SZETO
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Patent number: 11390888Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. in certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. in some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: GrantFiled: March 11, 2020Date of Patent: July 19, 2022Assignee: AMYRIS, INC.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Publication number: 20200354746Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. in certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. in some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: ApplicationFiled: March 11, 2020Publication date: November 12, 2020Applicant: Amyris, Inc.Inventors: Andrew HORWITZ, Kristy Michelle HAWKINS, Max SCHUBERT, Wayne SZETO
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Patent number: 10626418Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: GrantFiled: July 24, 2018Date of Patent: April 21, 2020Assignee: AMYRIS, INC.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Publication number: 20200115706Abstract: This invention provides methods of altering a cell including providing the cell with a nucleic acid sequence encoding a Cas1 protein and/or a Cas2 protein of a CRISPR adaptation system, providing the cell with a CRISPR array nucleic acid sequence including a leader sequence and at least one repeat sequence, and providing the cell with one or more retron systems, wherein the cell expresses the Cas1 protein and/or the Cas2 protein.Type: ApplicationFiled: April 12, 2018Publication date: April 16, 2020Inventors: Seth Lawler Shipman, Jeffrey Matthew Nivala, George M. Church, Max Schubert
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Publication number: 20190264196Abstract: Expressing guide nucleic acids (e.g., gRNA) from the same oligonucleotide that contains donor sequence permits the high efficiency, simultaneous transformation of a population of cells with both substrates. Using oligonucleotide chip array technology, one can construct thousands of oligonucleotides with customized gRNA and donor sequence in a cost effective manner. In combination, one can efficiently modify endogenous and exogenous genes.Type: ApplicationFiled: June 12, 2017Publication date: August 29, 2019Applicant: President and Fellows of Harvard CollegeInventors: Xiaoge Guo, Alejandro Chavez, Max Schubert, Eric Kelsic
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Publication number: 20190017074Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: ApplicationFiled: July 24, 2018Publication date: January 17, 2019Applicant: Amyris, Inc.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Patent number: 10041092Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: GrantFiled: September 9, 2016Date of Patent: August 7, 2018Assignee: AMYRIS, INC.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Publication number: 20180105832Abstract: The technology provided herein relates to novel methods and compounds for a multi-species pathogen infection control. In particular, the present disclosure pertains to methods of inhibiting the growth of a target pathogen expressing the cysteine-rich secreted protein (CSP), whereby the method comprises contacting said target pathogen with an inhibitor against said CSP, wherein said inhibitor inhibits the CSP expression and/or binds to a protein product of a gene coding CSP. Nucleic acid molecules encoding said inhibitors, vectors and host cells containing the nucleic acids and methods for preparation and producing such inhibitors are also disclosed, as well as the use of said CSP-inhibitors for the control/treatment of diseases associated with a microbial pathogen expressing CSP.Type: ApplicationFiled: April 13, 2015Publication date: April 19, 2018Inventors: Greta Noelke, Stefan Schillberg, Max Schubert
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Publication number: 20170058299Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: ApplicationFiled: September 9, 2016Publication date: March 2, 2017Applicant: Amyris, Inc.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Patent number: 9476065Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: GrantFiled: December 19, 2014Date of Patent: October 25, 2016Assignee: AMYRIS, INC.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Publication number: 20150184199Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: ApplicationFiled: December 19, 2014Publication date: July 2, 2015Applicant: AMYRIS, INC.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto