Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

Abstract: The present disclosure relates to the production of cannabinoids in either recombinant microorganism or in cell-free systems using a combination of enzymes, including but not limited to a PKS enzyme, a npgA enzyme, a cs-OLAS-1, a pp-DVAS-1, a cs-HEX-1 and/or Butiryl synthase.

Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

Abstract: A 3D printer comprising an extruder comprising a heated nozzle for depositing a layer of molten material on to a substrate, and a heating means configured to heat the substrate in an area underneath the heated nozzle of the extruder.

Abstract: A bioreactor is provided which contains cells capable of producing cytokine inhibitors in response to cytokines, in a manner regulated by the local or systemic milieu of an individual patient and predicted by mechanistic computational simulations. The bioreactor transfers the cytokine inhibitors to a patient in need of control of the inflammation process as part of a disease or condition in the patient, such as sepsis, trauma, traumatic brain injury, or wound healing. Related methods also are provided.

Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

Abstract: Provided are methods and systems for the visualization, distribution, and event-driven management of elements of a workflow that defines an analysis of very large data sets using multi-node compute clusters. A method for visualization of elements of such a workflow may include displaying the workflow via a user interface, collapsing groups of elements, adding further elements, removing elements, and modifying elements in the workflow. A digital workflow distribution platform comprises a user interface configured to allow a user to select a workflow, to acquire the workflow, to import the workflow into a user environment, and to develop the workflow imported into the user environment. An event-driven management engine for workflows may activate one or more computational modules in response to triggering events wherein the computational modules may be allocated non-sequentially in a distributed cloud computing environment to process a data set according to predetermined criteria.

Abstract: A bioreactor is provided which contains cells capable of producing cytokine inhibitors in response to cytokines, in a manner regulated by the local or systemic milieu of an individual patient and predicted by mechanistic computational simulations. The bioreactor transfers the cytokine inhibitors to a patient in need of control of the inflammation process as part of a disease or condition in the patient, such as sepsis, trauma, traumatic brain injury, or wound healing. Related methods also are provided.

Abstract: A bioreactor is provided which contains cells capable of producing cytokine inhibitors in response to cytokines, in a manner regulated by the local or systemic milieu of an individual patient and predicted by mechanistic computational simulations. The bioreactor transfers the cytokine inhibitors to a patient in need of control of the inflammation process as part of a disease or condition in the patient, such as sepsis, trauma, traumatic brain injury, or wound healing. Related methods also are provided.

Abstract: Biological pacemakers engineered to intrinsically generate rhythmic excitations are disclosed. In addition, methods of producing the biological pacemakers are disclosed. Methods of treating or preventing arrhythmia and heart disease associated with a defective pacemakers are also disclosed.

Abstract: Provided are methods and systems for the visualization, distribution, and event-driven management of elements of a workflow that defines an analysis of very large data sets using multi-node compute clusters. A method for visualization of elements of such a workflow may include displaying the workflow via a user interface, collapsing groups of elements, adding further elements, removing elements, and modifying elements in the workflow. A digital workflow distribution platform comprises a user interface configured to allow a user to select a workflow, to acquire the workflow, to import the workflow into a user environment, and to develop the workflow imported into the user environment. An event-driven management engine for workflows may activate one or more computational modules in response to triggering events wherein the computational modules may be allocated non-sequentially in a distributed cloud computing environment to process a data set according to predetermined criteria.

Abstract: Provided are methods and systems for scientific workflow distribution. A digital scientific workflow distribution platform comprises a user interface configured to allow a user to select a scientific workflow based on one or more parameters associated with the scientific workflow. Based on the selection, a distribution module enables acquiring the scientific workflow and importing the scientific workflow into a user environment. The digital scientific workflow distribution platform further comprises a management engine for scientific workflows configured to support development of the scientific workflow imported into the user environment.

Abstract: Provided are methods and systems for the visualization of elements of a scientific workflow. A method for visualization of elements of a scientific workflow may include displaying the scientific workflow via a user interface. Based on predetermined grouping criteria, one or more collapsible groups of elements are defined within the scientific workflow. Upon receiving a request to collapse the collapsible groups of elements, the collapsible groups of elements are collapsed into collapsed groups of elements. After collapsing, a layout of the plurality of elements and the collapsed groups of elements is selectively readjusted. The method can further include adding further elements to the scientific workflow, removing elements from the scientific workflow, and modifying elements in the scientific workflow.

Abstract: Provided are methods and systems for computer-implemented event-driven management of scientific workflows. An event-driven management engine for scientific workflows may comprise a decision node configured to determine that at least one condition within a scientific workflow is true by running a conditional loop. Based on the determination, the decision node may selectively activate a computational module. The event-driven management engine for scientific workflows may further comprise a fork-join queuing cluster. The fork-join queuing cluster may allocate the computational module non-sequentially to participant computational nodes in a distributed cloud computing environment and process a data set according to predetermined criteria. A distributed database of the event-driven management engine for scientific workflows may store the computational modules and conditions associated with the at least one computational module.

Abstract: Provided are methods and systems for the visualization of elements of a scientific workflow. A method for visualization of elements of a scientific workflow may include displaying the scientific workflow via a user interface. Based on predetermined grouping criteria, one or more collapsible groups of elements are defined within the scientific workflow. Upon receiving a request to collapse the collapsible groups of elements, the collapsible groups of elements are collapsed into collapsed groups of elements. After collapsing, a layout of the plurality of elements and the collapsed groups of elements is selectively readjusted. The method can further include adding further elements to the scientific workflow, removing elements from the scientific workflow, and modifying elements in the scientific workflow.

Abstract: Provided are methods and systems for scientific workflow distribution. A digital scientific workflow distribution platform comprises a user interface configured to allow a user to select a scientific workflow based on one or more parameters associated with the scientific workflow. Based on the selection, a distribution module enables acquiring the scientific workflow and importing the scientific workflow into a user environment. The digital scientific workflow distribution platform further comprises a management engine for scientific workflows configured to support development of the scientific workflow imported into the user environment.

Abstract: A bioreactor is provided which contains cells capable of producing cytokine inhibitors in response to cytokines, in a manner regulated by the local or systemic milieu of an individual patient and predicted by mechanistic computational simulations. The bioreactor transfers the cytokine inhibitors to a patient in need of control of the inflammation process as part of a disease or condition in the patient, such as sepsis, trauma, traumatic brain injury, or wound healing. Related methods also are provided.