Abstract: A connection device between two micro-channels or two milli-channels of two fluidic elements including a complementary arrangement for holding the device with at least one of the two fluidic elements. A micro-fluidic or milli-fluidic chip including a connection device between a micro-channel or a milli-channel of the chip and a micro-channel or a milli-channel of a fluidic element.

Abstract: The invention relates to a three-dimensional cellular microcompartment or an assembly of three-dimensional cellular microcompartments of ovoid, cylindrical, spheroid or spherical shape, or substantially ovoid, cylindrical, spheroid or spherical shape, comprising an external hydrogel layer defining an internal part (14), said internal part comprising at least: extracellular matrix elements, and at least two cysts, each cyst being formed by at least one layer of human or animal cells organised three-dimensionally around a lumen, the smallest radius or the average radius of the internal part being at least 100 ?m. The invention also relates to a method for producing such a microcompartment or microcompartment assembly.

Abstract: A cellular microcompartment comprising: at least one layer of cells, an outer layer of hydrogel, and a fibrin mesh arranged between the outer layer of hydrogel and said layer of cells, the cellular microcompartment being compliant with regulations for Good Manufacturing Practices (GMP) and suitable for clinical applications, as well as methods for preparing the cellular microcompartment, and methods in which the cellular microcompartment is used for production of cells and/or tissues, including a large-scale production.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

Abstract: The invention relates to a compacted tissue of human cardiac cells expressing cardiac troponin C, which is contractile and has a low spontaneous contraction frequency. The invention also relates to the use of the tissue, in particular in the treatment of a cardiac pathology, particularly ischaemic heart disease.

Abstract: The invention relates to a liver microtissue, preferably, the liver microtissue having the largest dimension between 500 and 700 ?m, and the liver microtissue expressing CYP3A4 monooxygenase with an activity of at least 75,000 RLU per million cells and producing at least 18 ag of urea per million cells per 24 hours. The invention also relates to a method for producing the liver microtissue from pluripotent stem cells, and uses of the liver microtissue in treatment and/or prevention of liver failure.

Abstract: The invention relates to cellular microcompartments, each microcompartment successively comprising the following layers, which are organised around at least one lumen: —at least one inner layer of human cells undergoing cardiac differentiation, expressing at least one gene chosen from PDGFR?, MESP-1, NKX2-5, GATA4, MEF2C, TBX20, ISL1 and TBX5, —at least one intermediate layer of isotonic aqueous solution, and—at least one outer hydrogel layer. The invention also relates to the cardiac tissues obtained from said microcompartments and the use thereof, particularly in the treatment of heart disease.

Abstract: The invention relates to a liver microtissue, preferably a liver microtissue comprising at least 3 different phenotypes of liver cells obtained from induced pluripotent stem cells encapsulated in a single three-dimensional closed microcompartment. The invention also relates to a method for preparing the liver microtissue and its uses in treatment or prevention of liver failure.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

Abstract: The invention relates to a neural tissue unit for use in implantation into the nervous system of a human or non-human mammal, wherein said neural tissue unit contains differentiated post-mitotic neuronal cells in an extracellular matrix, said unit being obtained from a cellular microcompartment comprising a hydrogel capsule surrounding the neural tissue unit, and said hydrogel capsule being at least partially removed before use of the neural tissue unit. The invention also relates to a process for preparing such a neural tissue unit.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

Abstract: The invention relates to a process for producing a plurality of capsules, each comprising an external shell of crosslinked hydrogel surrounding a central core, according to which a hydrogel solution and a composition of interest, intended to form the central core, are concentrically co-extruded so as to form mixed drops, each comprising a layer of hydrogel solution surrounding a drop of liquid composition of interest, characterized in that the co-extrusion step is carried out above a crosslinking aerosol so that the mixed drops pass through said crosslinking aerosol, such that the layer of hydrogel solution at least partially crosslinks around the drop of liquid composition of interest on contact with said aerosol.

Abstract: The present invention relates to a method for in-vitro production of mammalian neurons expressing the 6 isoforms of the Tau protein (2N4R, 1N4R, 0N4R, 2N3R, 1N3R, 0N3R), comprising a step of neuronal differentiation, in which cellular microcompartments are cultivated for a period of 5 weeks to 100 weeks, each one comprising a hollow hydro gel capsule surrounding post-mitotic neuronal cells and an extracellular matrix, the neuronal differentiation step being carried out in a bioreactor, the cellular microcompartments being kept in suspension in an enclosure of the bioreactor containing a neuronal differentiation medium.

Abstract: The invention relates to three-dimensional tissue units which are hollow and which comprise, when organized about an internal opening, at least one layer of living human retinal pigment epithelium cells which are differentiated, the basal face of each cell pointing outwards and the apical face pointing towards the internal opening. The invention also relates to these tissue units for use in the treatment of retinopathies, and to a method for preparing these tissue units and an implantation kit.

Abstract: The invention relates to a bioreactor cell culture system comprising a closed chamber containing a plurality of suspended cell microcompartments, wherein the microcompartments each comprise an outer hydrogel layer providing a cavity containing a set of self-organized cells and extracellular matrix or an extracellular matrix substitute. The invention further relates to the use of such bioreactors to produce cells and/or organoids, and/or molecules and/or complex molecular assemblies.

Abstract: The invention relates to a process for producing a plurality of capsules, each comprising an external shell of crosslinked hydrogel surrounding a central core, according to which a hydrogel solution and a composition of interest, intended to form the central core, are concentrically co-extruded so as to form mixed drops, each comprising a layer of hydrogel solution surrounding a drop of liquid composition of interest, characterized in that the co-extrusion step is carried out above a crosslinking aerosol so that the mixed drops pass through said crosslinking aerosol, such that the layer of hydrogel solution at least partially crosslinks around the drop of liquid composition of interest on contact with said aerosol.

Abstract: The invention relates to a neural tissue unit for use in implantation into the nervous system of a human or non-human mammal, wherein said neural tissue unit contains differentiated post-mitotic neuronal cells in an extracellular matrix, said unit being obtained from a cellular microcompartment comprising a hydrogel capsule surrounding the neural tissue unit, and said hydrogel capsule being at least partially removed before use of the neural tissue unit. The invention also relates to a process for preparing such a neural tissue unit.

Abstract: The invention relates to a hollow cell microfibre comprising successively, organized around a lumen, at least one endothelial cell layer, at least one smooth muscle cell layer, an extracellular matrix layer, and optionally an outer hydrogel layer. The invention also relates to a process for fabricating such a hollow cell microfibre.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.