Abstract: Systems and methods are disclosed for generating an elastic stiffness map for a volume of an ophthalmic tissue of an eye of a patient. Exemplary systems and methods involve a laser assembly, an optical scanning assembly, an objective lens assembly, a beam control assembly, an eye camera assembly, and a Brillouin spectrometer assembly. Systems and methods can operate to transmit x,y coordinate scan control signals to the optical scanning assembly, transmit z coordinate scan control signals to the objective lens assembly, and generate the elastic stiffness map for the volume of the ophthalmic tissue of the eye based on Brillouin signals generated by a Brillouin spectrometer of the Brillouin spectrometer assembly.

Abstract: Input light comprising an optical signal associated with a spectrum of at least a portion of the input light is received into an interferometer. Data comprising an image is provided using a detector array comprising detection elements. Output light is received from the interferometer into a set of one or more optical elements, which provide an optical interference pattern associated with an intensity of at least a portion of the output light that is distributed over a set of detection elements. Detection of the optical signal is stabilized based at least in part on the data using a control system comprising: a light-control module that controls a first feedback loop that stabilizes the portion of the output light; and/or a temperature-control module that controls a second feedback loop that stabilizes a temperature sensed by at least one temperature sensor within a thermal environment associated with the interferometer.

Abstract: Systems and methods are disclosed for generating an elastic stiffness map for a volume of an ophthalmic tissue of an eye of a patient. Exemplary systems and methods involve a laser assembly, an optical scanning assembly, an objective lens assembly, a beam control assembly, an eye camera assembly, and a Brillouin spectrometer assembly. Systems and methods can operate to transmit x,y coordinate scan control signals to the optical scanning assembly, transmit z coordinate scan control signals to the objective lens assembly, and generate the elastic stiffness map for the volume of the ophthalmic tissue of the eye based on Brillouin signals generated by a Brillouin spectrometer of the Brillouin spectrometer assembly.

Abstract: An apparatus and method for providing a gradient strain to an extracellular matrix to induce mechanotransduction. The gradient strain can be produced by adhering the extracellular matrix to a post and the walls of a sample holder, and then rotating the post and the sample holder. This strain can result in increased stiffness of the extracellular matrix with very little loss of pore size.

Abstract: An apparatus and method for providing a gradient strain to an extracellular matrix to induce mechanotransduction. The gradient strain can be produced by adhering the extracellular matrix to a post and the walls of a sample holder, and then rotating the post and the sample holder. This strain can result in increased stiffness of the extracellular matrix with very little loss of pore size.