Abstract: The present invention relates to a method for refining an ophthalmic protein pharmaceutical with an isoelectric point in a particular range by applying cation exchange chromatography or anion exchange chromatography. According to the present invention, high-purity ophthalmic protein pharmaceutical can be produced more economically by applying three or four chromatography processes.

Abstract: A refrigerator having an ice crush apparatus enables to conveniently prepare food such as shaved ice with sugar syrup includes a motor and a section formed by plural links for transferring the motor in the axial direction. A clutch is installed between the motor and a freezing container which is formed with a clutch disc onto the bottom plane, and a rotating rod and brackets are installed for reversing the freezing container. A cutter is fixed to face the lower portion of the reversed to be rotated freezing container for crushing the ice within the freezing container. That is, the ice is crushed by the cutter while the freezing container is reversely rotated and transferred downward to be the food such as shaved ice with sugar syrup. Thus, the water is frozen to be crushed for preparing the shaved ice with sugar syrup simply and conveniently within the household refrigerator. Also, the shaved ice with sugar syrup can be prepared without taking out or relocating the ice to have an effect for sanitary reason.