Patents by Inventor Megumi KUROKAWA

Megumi KUROKAWA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing proteins
    Patent number: 9410176
    Abstract: This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.
    Type: Grant
    Filed: December 14, 2011
    Date of Patent: August 9, 2016
    Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTD.
    Inventors: Megumi Kurokawa, Yoko Hayashi, Masayoshi Tsukahara
  • Process for production of protein
    Patent number: 9102971
    Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: August 11, 2015
    Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTD
    Inventors: Megumi Kurokawa, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara, Koichi Kawakami, Yoko Hayashi
  • METHOD FOR PRODUCING PROTEINS
    Publication number: 20130273604
    Abstract: This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.
    Type: Application
    Filed: December 14, 2011
    Publication date: October 17, 2013
    Applicant: KYOWA HAKKO KIRIN CO., LTD.
    Inventors: Megumi Kurokawa, Yoko Hayashi, Masayoshi Tsukahara
  • PROCESS FOR PRODUCTION OF PROTEIN
    Publication number: 20120196327
    Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.
    Type: Application
    Filed: December 15, 2011
    Publication date: August 2, 2012
    Applicants: Kyowa Hakko Kirin Co., Ltd., Inter-University Research Institute Corporation Research Organization of Information and Systems
    Inventors: Megumi KUROKAWA, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA, Koichi KAWAKAMI, Yoko HAYASHI