Patents by Inventor Megumi Shigematsu

Megumi Shigematsu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • SPECIFIC EXPRESSION OF HALF-TRNA IN CANCERS
    Publication number: 20210355548
    Abstract: The present invention relates to systems, devices and methods for diagnosing cancer. In various embodiments, the present invention provides a method for performing sequencing of 5?-htRNA in an RNA sample including obtaining a DNA library of 5?-htRNA and sequencing the DNA library of 5?-htRNAs in the RNA sample. The invention also teaches a method for quantifying 5?-htRNA half molecules in an RNA sample including (a) treating an RNA sample containing 5?-htRNAs half molecules having a 3? cyclic phosphate with a T4 polynucleotide kinase to form 3?-dephosphrylated 5?-tRNA halves, (b) 3?-AD ligating the 3?-dephosphrylated 5?-tRNA halves to form ligated RNA products, and (c) quantifying the ligated RNA products by RT-qPCR using a plurality of target primers and probes configured to simultaneously quantify at least two cP-containing 5?-tRNA half species. The treating of step (a) is simultaneously performed as one step in a single tube with the 3?-AD ligating of step (b).
    Type: Application
    Filed: May 6, 2021
    Publication date: November 18, 2021
    Applicant: Thomas Jefferson University
    Inventors: Yohei Kirino, Shozo Honda, Megumi Shigematsu