Abstract: A device including a pressure generating assembly and a chamber for generating hydrostatic pressure in the said chamber. In particular, the device is useful for cell mechanical stimulation and tissue regeneration by generating hydrostatic pressure in a chamber receiving samples such as cell cultures, biological tissues, and cell seeded biomaterial constructs for the purpose of treatment with hydrostatic pressure. The pressure generating assembly of the device comprises a piston and a cylinder generating and relieving pressure by moving the piston in the cylinder using an actuating system for quick placement and removal of the piston in and out of the cylinder. The device is portable and autoclavable and can be hand operated without any tool to generate pressures at high physiological levels up to at least 10 MPa. The device has a venting system allowing vital gasses to reach the samples under treatment when the device is placed in an incubator.

Abstract: A fixation mechanism for use in a prosthesis implantable into bone comprises a main body defining an internal passageway and including a plurality of openings extending between the internal passageway and the exterior of the main body. The device has a plurality of bone engaging members, with each bone engaging member being mounted in a respective opening for movement between a retracted position in which the bone engaging member does not extend outside the body, and an extended position in which the bone engaging member extends through the respective opening into surrounding bone to secure the device. A plunger within the main body is adapted to directly or indirectly engage the bone engaging members to displace the bone engaging members out through their respective openings.