Abstract: A detection device includes a carrying unit, at least a reaction unit, a wire transporting unit, and a guiding unit. The carrying unit includes at least a hole, and the reaction unit is disposed on one side of the hole. The reaction unit includes at least a reaction area, which includes a hydrophilic material containing a detection reagent. The wire transporting unit is disposed on the other side of the hole and includes a sampling end and a transporting end. The guiding unit is in contact with the reaction unit and the transporting end. The guiding unit collects a test sample through the transporting end and provides at least a part of the test sample to react with the detection reagent. The detection device adopts the wire transporting unit to replace the pipette for collecting the test sample without directly contacting the detection area with the sample source.