Patents by Inventor Melanie Dart

Melanie Dart has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Luciferase-based thermal shift assays
    Patent number: 11899021
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: January 4, 2023
    Date of Patent: February 13, 2024
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION
    Publication number: 20230342826
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Application
    Filed: November 7, 2022
    Publication date: October 26, 2023
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • LUCIFERASE-BASED THERMAL SHIFT ASSAYS
    Publication number: 20230341410
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Application
    Filed: January 4, 2023
    Publication date: October 26, 2023
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • COMPOSITIONS AND METHODS FOR STABILIZING COELENTERAZINE AND ANALOGS AND DERIVATIVES THEREOF
    Publication number: 20230092932
    Abstract: Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.
    Type: Application
    Filed: November 21, 2022
    Publication date: March 23, 2023
    Inventors: Melanie Dart, Thomas Smith, Thomas Kirkland, Thomas Machleidt, Keith Wood
  • Luciferase-based thermal shift assays
    Patent number: 11567083
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: November 25, 2020
    Date of Patent: January 31, 2023
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • Compositions and methods for stabilizing coelenterazine and analogs and derivatives thereof
    Patent number: 11534504
    Abstract: Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.
    Type: Grant
    Filed: October 3, 2019
    Date of Patent: December 27, 2022
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Thomas Smith, Thomas Kirkland, Thomas Machleidt, Keith Wood
  • Activation of bioluminescene by structural complementation
    Patent number: 11493504
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Grant
    Filed: April 17, 2020
    Date of Patent: November 8, 2022
    Assignee: Promega Corporation
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • REACTIVE PEPTIDE LABELING
    Publication number: 20220065786
    Abstract: Provided herein are sulfo n-hydroxysuccimidyl ester (sulfo-SE) linked peptides, methods of synthesis thereof, and methods of using such peptides for labeling of biomolecules. In particular, peptides comprising non-alkyl group such as serine, threonine, cysteine, tyrosine, glutamic acid, and aspartic acid are stably modified (e.g., without autoreactivity) with a sulfo-SE group and used to label or otherwise modify biomolecules.
    Type: Application
    Filed: November 9, 2021
    Publication date: March 3, 2022
    Inventors: Hui Wang, Nidhi Nath, Rod Flemming, Becky Godat, Wenhui Zhou, Virginia Kincaid, Melanie Dart
  • Reactive peptide labeling
    Patent number: 11175228
    Abstract: Provided herein are sulfo n-hydroxysuccimidyl ester (sulfo-SE) linked peptides, methods of synthesis thereof, and methods of using such peptides for labeling of biomolecules. In particular, peptides comprising non-alkyl group such as serine, threonine, cysteine, tyrosine, glutamic acid, and aspartic acid are stably modified (e.g., without autoreactivity) with a sulfo-SE group and used to label or otherwise modify biomolecules.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: November 16, 2021
    Inventors: Hui Wang, Nidhi Nath, Rod Flemming, Becky Godat, Wenhui Zhou, Virginia Kincaid, Melanie Dart
  • MULTIPARTITE LUCIFERASE PEPTIDES AND POLYPEPTIDES
    Publication number: 20210262941
    Abstract: Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.
    Type: Application
    Filed: November 27, 2020
    Publication date: August 26, 2021
    Inventors: Virginia Kincaid, Lance P. Encell, Mary Hall, Michael Killoran, Keith Wood, Melanie Dart, Trish Hoang
  • LUCIFERASE-BASED THERMAL SHIFT ASSAYS
    Publication number: 20210080470
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Application
    Filed: November 25, 2020
    Publication date: March 18, 2021
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • Luciferase-based thermal shift assays
    Patent number: 10928400
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: February 11, 2020
    Date of Patent: February 23, 2021
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • COMPOSITIONS AND METHODS FOR ANALYTE DETECTION USING BIOLUMINESCENCE
    Publication number: 20200386748
    Abstract: Provided herein are systems and methods for the detection of an analyte or analytes in a sample. In particular, the present disclosure provides compositions, assays, and methods for detecting and/or quantifying a target analyte using a bioluminescent complex comprising substrates, peptides, and/or polypeptides capable of generating a bioluminescent signal that correlates to the presence, absence, or amount of the target analyte.
    Type: Application
    Filed: April 10, 2020
    Publication date: December 10, 2020
    Inventors: Thomas Kirkland, Melanie Dart, Zhiyang Zeng, Thomas Smith, Keith Wood, Thomas Machleidt, Virginia Kincaid, Hui Wang, Wenhui Zhou, Emily Jost, Lance Encell, Mary Hall, Robin Hurst
  • ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION
    Publication number: 20200292534
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Application
    Filed: April 17, 2020
    Publication date: September 17, 2020
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • MULTIPARTITE LUCIFERASE
    Publication number: 20200270586
    Abstract: Provided herein are compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular, a bioluminescent complex is formed upon the interaction of two or more peptide tags (e.g., separately or fused as a dipeptide or tripeptide) and a polypeptide component.
    Type: Application
    Filed: June 12, 2019
    Publication date: August 27, 2020
    Inventors: Mary Hall, Lance P. Encell, Michael Killoran, Keith Wood, Thomas Smith, Virginia Kincaid, Thomas Kirkland, Melanie Dart
  • LUCIFERASE-BASED THERMAL SHIFT ASSAYS
    Publication number: 20200174012
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Application
    Filed: February 11, 2020
    Publication date: June 4, 2020
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • REACTIVE PEPTIDE LABELING
    Publication number: 20200166460
    Abstract: Provided herein are sulfo n-hydroxysuccimidyl ester (sulfo-SE) linked peptides, methods of synthesis thereof, and methods of using such peptides for labeling of biomolecules. In particular, peptides comprising non-alkyl group such as serine, threonine, cysteine, tyrosine, glutamic acid, and aspartic acid are stably modified (e.g., without autoreactivity) with a sulfo-SE group and used to label or otherwise modify biomolecules.
    Type: Application
    Filed: November 27, 2019
    Publication date: May 28, 2020
    Inventors: Hui Wang, Nidhi Nath, Rod Flemming, Becky Godat, Wenhui Zhou, Virginia Kincaid, Melanie Dart
  • Activation of bioluminescence by structural complementation
    Patent number: 10648971
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: May 12, 2020
    Assignee: Promega Corporation
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • COMPOSITIONS AND METHODS FOR STABILIZING COELENTERAZINE AND ANALOGS AND DERIVATIVES THEREOF
    Publication number: 20200109146
    Abstract: Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.
    Type: Application
    Filed: October 3, 2019
    Publication date: April 9, 2020
    Inventors: Melanie Dart, Thomas Smith, Thomas Kirkland, Thomas Machleidt, Keith Wood
  • Luciferase-based thermal shift assays
    Patent number: 10571471
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: February 25, 2020
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi