Abstract: The present invention provides a method for notifying at least one invitee of an initial event of changes to the initial event. The method comprises receiving the initial event comprising a plurality of initial event elements and receiving an updated event comprising a plurality of updated event elements, each updated event element corresponding to a respective initial event element. The updated event elements are compared to the initial event elements to determine which of the updated event elements are changed event elements that differ from their respective initial event elements and the at least one invitee is notified of any specific changed event elements.

Abstract: This invention provides methods to prepare and use immunostimulatory cells for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-?R) agonist and/or a tumor necrosis factor alpha receptor (TNF-?R) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g.