Abstract: Compositions of polynucleotide(s), pharmaceutical compositions thereof, and methods of use thereof are disclosed. A polynucleotide may be or encode a synthetic transfer ribonucleic acid (tRNA). The polynucleotide may be assembled with a lipid composition for delivery to a cell or an organ, such as a lung cell or a lung of a subject. Methods for enhancing an expression or activity of cystic fibrosis transmembrane conductance regulator (CFTR) protein in a cell are provided. Methods for treating a subject having or suspected of having a CFTR-associated condition are also provided.

Abstract: Provided herein are isolated nucleic acids comprising two or more nucleotide sequences capable of encoding genome-editing enzymes. Also provided are vectors comprising the isolated nucleic acids, host cells comprising the vectors, and kits comprising the isolated nucleic acids.