Abstract: A chromatographic apparatus includes two chromatographic columns configured to perform chromatography. The apparatus further includes a primary valve disposed upstream of the first chromatographic column and the second chromatographic column. The primary valve selectively switches between a first position in which the pump is in fluid communication with a first path that includes the first chromatographic column and a second position in which the pump is in fluid communication with a second path that includes the second chromatographic column. The apparatus also includes a first and a second sample injection port associated with said first and second columns, respectively, and at least one detector. The apparatus is configured such that flow from the column in the selected path goes to said at least one detector without passing through the other column.

Abstract: Pump priming systems and chromatography systems containing the same are, disclosed. Methods of making and methods of using pump priming systems are also disclosed. In conventional chromatography instrumentation, pump priming is performed in a manual mode where the operator manually opens a mechanical valve and uses a syringe to move liquid through the pump until the lines and the pump are filled with liquid.