Abstract: The present invention provides an adenoviral-mediated method of transfection with nucleic acids which can be augmented through incubation of the nucleic acids with cationic agents. Specifically, the present inventive method of introducing a nucleic acid into a eukaryotic cell comprises contacting the cell with, in any order or simultaneously, the nucleic acid and an adenovirus, wherein the nucleic acid is not bound to any molecule capable of effecting its entry into the cell. The cell is preferably additionally contacted with a cationic agent, such as a monocationic or polycationic liposome, such that the nucleic acid is not bound to any molecule capable of effecting its entry into the cell other than, optionally, the cationic agent.