Patents by Inventor Melud Nabavi
Melud Nabavi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220411458Abstract: Phosphoramidate-based monomers are provided for use in the synthesis of expandable polymers for nanopore-based sensing. Such monomers comprising a reporter construct that contain a first reporter code, a symmetrical chemical brancher bearing a translocation control element, and a second reporter code, wherein the ends of the reporter construct are attached to phosphoramidate-nucleoside. Related methods and products are also provided.Type: ApplicationFiled: November 23, 2021Publication date: December 29, 2022Inventors: Brent Banasik, Cynthia Cech, Jagadeeswaran Chandrasekar, Drew Goodman, Aaron Jacobs, Mark Stamatios Kokoris, Svetlana Kritzer, Matthew Lopez, Cara Machacek, Lacey Merrill, Melud Nabavi, Dylan O'Connell, John Tabone, Jessica Vellucci
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Publication number: 20220186205Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.Type: ApplicationFiled: March 3, 2022Publication date: June 16, 2022Inventors: Mark Stamatios Kokoris, Marc Prindle, Melud Nabavi, Craig Ostrander, Taylor Lehmann, Samantha Vellucci, Michael Kovarik, Jack Chase, Robert Busam, Miranda Lahman
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Publication number: 20220112548Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.Type: ApplicationFiled: December 21, 2021Publication date: April 14, 2022Inventors: Jagadeeswaran Chandrasekar, Drew Goodman, Aaron Jacobs, Mark Stamatios Kokoris, Lacey Merrill, Melud Nabavi, Dylan O'Connell, John Tabone
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Patent number: 11299725Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.Type: GrantFiled: May 4, 2020Date of Patent: April 12, 2022Assignee: Stratos Genomics, Inc.Inventors: Mark Stamatios Kokoris, Marc Prindle, Melud Nabavi, Craig Ostrander, Taylor Lehmann, Samantha Vellucci, Michael Kovarik, Jack Chase, Robert Busam, Miranda Lahman
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Publication number: 20220073556Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.Type: ApplicationFiled: March 18, 2021Publication date: March 10, 2022Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Aaron Jacobs
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Publication number: 20210164016Abstract: Methods and materials are provided for detecting nucleic acid sequence differences including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions in single molecule target members present in a test population of nucleic acid fragments. Heteroduplexes are formed between members of the test nucleic acid population and their corresponding complements provided in a pool of mismatch cleavage probes. Mismatched base pairs in the heteroduplexes are specifically cleaved and cleaved probe fragments are electronically detected to signal the present of the target members in the test population.Type: ApplicationFiled: August 3, 2018Publication date: June 3, 2021Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Cara Machacek
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Publication number: 20210139966Abstract: The invention relates to methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.Type: ApplicationFiled: December 20, 2018Publication date: May 13, 2021Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Aaron Jacobs, Dylan O'Connell, Drew Goodman, Lacey Merrill, Jagadeeswaran Chandrasekar
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Publication number: 20210062251Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.Type: ApplicationFiled: December 27, 2018Publication date: March 4, 2021Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Aaron Jacobs, Dylan O'Connell, Drew Goodman, Lacey Merrill, Jagadeeswaran Chandrasekar, Kendall Berg, Samantha Vellucci, Jessica Vellucci
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Publication number: 20200385701Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.Type: ApplicationFiled: May 4, 2020Publication date: December 10, 2020Inventors: Mark Stamatios Kokoris, Marc Prindle, Melud Nabavi, Craig Ostrander, Taylor Lehmann, Samantha Vellucci, Michael Kovarik, Jack Chase, Robert Busam, Miranda Lahman
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Patent number: 10774105Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed, including compounds having the following structure: wherein R1, R2, R3 and R4 are as defined herein. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.Type: GrantFiled: April 10, 2019Date of Patent: September 15, 2020Assignee: STRATOS GENOMICS, INC.Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Aaron Jacobs
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Patent number: 10745685Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.Type: GrantFiled: November 11, 2016Date of Patent: August 18, 2020Assignee: Stratos Genomics, Inc.Inventors: Mark Stamatios Kokoris, Marc Prindle, Melud Nabavi, Craig Ostrander, Taylor Lehmann, Samantha Vellucci, Michael Kovarik, Jack Chase, Robert Busam, Miranda Lahman
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Publication number: 20190330254Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.Type: ApplicationFiled: April 10, 2019Publication date: October 31, 2019Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Aaron Jacobs
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Patent number: 10301345Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed, including compounds having the following structure: wherein R1, R2, R3 and R4 are as defined herein.Type: GrantFiled: November 20, 2015Date of Patent: May 28, 2019Assignee: STRATOS GENOMICS, INC.Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Aaron Jacobs
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Publication number: 20190078075Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.Type: ApplicationFiled: November 11, 2016Publication date: March 14, 2019Inventors: Mark Stamatios Kokoris, Marc Prindle, Melud Nabavi, Craig Ostrander, Taylor Lehmann, Samantha Vellucci, Michael Kovarik, Jack Chase, Robert Busam, Miranda Lahman
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Publication number: 20160145292Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.Type: ApplicationFiled: November 20, 2015Publication date: May 26, 2016Inventors: Mark Stamatios Kokoris, John Tabone, Melud Nabavi, Aaron Jacobs
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Patent number: 8772017Abstract: Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.Type: GrantFiled: June 8, 2012Date of Patent: July 8, 2014Assignee: Micronics, Inc.Inventors: C. Frederick Battrell, John Gerdes, John R. Williford, Denise Maxine Hoekstra, Wayne L. Breidford, Stephen Mordue, John Clemmens, Melud Nabavi, Mark Kokoris
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Publication number: 20120329142Abstract: Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.Type: ApplicationFiled: June 8, 2012Publication date: December 27, 2012Applicant: MICRONICS, INC.Inventors: C. Frederick BATTRELL, John GERDES, John R. WILLIFORD, Denise Maxine HOEKSTRA, Wayne L. BREIDFORD, Stephen MORDUE, John CLEMMENS, Melud NABAVI, Mark KOKORIS
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Patent number: 8222023Abstract: Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.Type: GrantFiled: September 10, 2008Date of Patent: July 17, 2012Assignee: Micronics, Inc.Inventors: C. Frederick Battrell, John Gerdes, John R. Williford, Denise Maxine Hoekstra, Wayne L. Breidford, Stephen Mordue, John Clemmens, Melud Nabavi, Mark Kokoris
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Publication number: 20090148933Abstract: Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.Type: ApplicationFiled: September 10, 2008Publication date: June 11, 2009Applicant: Micronics, Inc.Inventors: C. Frederick Battrell, John Gerdes, John R. Williford, Denise Maxine Hoekstra, Wayne L. Breidford, Stephen Mordue, John Clemmens, Melud Nabavi, Mark Kokoris
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Publication number: 20090148847Abstract: Disclosed is an improvement in methods for nucleic acid and immunological bioassays. The methods comprise a step for “sweeping” paramagnetic bead: target molecule complexes so as to capture them with an affinity capture agent on a test pad by moving a magnetic force field from outside to inside the test pad area so as to bring into contact the paramagnetic complexes with the capture agent, while sweeping any unbound paramagnetic material off the test pad by moving the magnetic field from inside to outside the test pad area. Surprisingly, the paramagnetic complexes are rapidly affinity-extracted from the moving magnetic field.Type: ApplicationFiled: September 3, 2008Publication date: June 11, 2009Applicant: MICRONICS, INC.Inventors: Mark Kokoris, Melud Nabavi, Wayne L. Breidford, John Gerdes, Stephen Mordue, C. Frederick Battrell