Abstract: The present disclosure relates to tagged nucleoside-5?-oligophosphates having a positively charged polymer tag structure and components thereof. Such nucleoside-5?-oligophosphates are useful, for example, in nanopore-based sequencing-by-synthesis applications. Also disclosed herein are nanopore constructs engineered to have additional negatively-charged moieties in the channel of the nanopore. Such nanopores can be useful, for example, for providing a repellant force against template and/or primer nucleic acids inserting into the pore during a nucleic sequence-by-synthesis process. The tagged nucleoside-5?-oligophosphates and nanopores disclosed herein can be used together to provide nanopore-based nucleic acid sequencing-by-synthesis systems and processes having reduced background tag levels and improved throughput.

Abstract: The method comprises of placing the palm side of a glove mold facing up, and raising the fingers portion of glove mold up, in such a way that the glove mold is tilting up at 15°-45° in relative to horizontal position. The first pouring process is carried out by pouring silica gel liquid on the fingers portion of glove mold. Then adjust the glove mold to horizontal position, and rotate the glove mold by using its length as axis. Continue pouring silica gel liquid on the glove mold surface to carry out second pouring process. Let the silica gel liquid to be coated on the whole glove mold surface forming a semi-finished glove. Upon completion, let the glove mold to go through a dripping procedure for dripping treatment. After the dripping treatment, carry out vulcanization and cooling to obtain the corresponding silica gel glove.

Abstract: The method comprises of placing the palm side of a glove mold facing up, and raising the fingers portion of glove mold up, in such a way that the glove mold is tilting up at 15°-45° in relative to horizontal position. The first pouring process is carried out by pouring silica gel liquid on the fingers portion of glove mold. Then adjust the glove mold to horizontal position, and rotate the glove mold by using its length as axis. Continue pouring silica gel liquid on the glove mold surface to carry out second pouring process. Let the silica gel liquid to be coated on the whole glove mold surface forming a semi-finished glove. Upon completion, let the glove mold to go through a dripping procedure for dripping treatment. After the dripping treatment, carry out vulcanization and cooling to obtain the corresponding silica gel glove.