Patents by Inventor Meng Taing
Meng Taing has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240150824Abstract: The present disclosure relates to tagged nucleoside-5?-oligophosphates having a positively charged polymer tag structure and components thereof. Such nucleoside-5?-oligophosphates are useful, for example, in nanopore-based sequencing-by-synthesis applications. Also disclosed herein are nanopore constructs engineered to have additional negatively-charged moieties in the channel of the nanopore. Such nanopores can be useful, for example, for providing a repellant force against template and/or primer nucleic acids inserting into the pore during a nucleic sequence-by-synthesis process. The tagged nucleoside-5?-oligophosphates and nanopores disclosed herein can be used together to provide nanopore-based nucleic acid sequencing-by-synthesis systems and processes having reduced background tag levels and improved throughput.Type: ApplicationFiled: August 9, 2021Publication date: May 9, 2024Inventors: Aruna Ayer, Frank Bergmann, Peter Crisalli, Lars Hillringhaus, Omid Khakshoor, Hannes Kuchelmeister, Rongxin Nie, Marshall W. Porter, Christoph Seidel, Seong-Ho Shin, Meng Taing, Adolfo Vargas
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Publication number: 20240010668Abstract: The present disclosure provides 3? protected nucleotides, including those 3? protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3? protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.Type: ApplicationFiled: August 11, 2023Publication date: January 11, 2024Inventors: Frank Bergmann, Peter Crisalli, Dieter Heindl, Omid Khakshoor, Meng Taing
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Patent number: 11773130Abstract: The present disclosure provides 3? protected nucleotides, including those 3? protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3? protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.Type: GrantFiled: July 6, 2022Date of Patent: October 3, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Frank Bergmann, Peter Crisalli, Dieter Heindl, Omid Khakshoor, Meng Taing
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Publication number: 20230159999Abstract: This application discloses compositions comprising primer compounds that reduce or block deleterious threading into a nanopore of nucleic acid strands displaced by a nanopore-linked polymerase, for example during the use of a nanopore device for nucleic acid sequencing. Also disclosed are methods for using the compositions to reduce deleterious threading events during nanopore-based nucleic acid detection techniques, such as nanopore sequencing.Type: ApplicationFiled: August 4, 2022Publication date: May 25, 2023Inventors: Aruna Ayer, David Beyer, Peter Crisalli, Kirti Dhiman, Helen Franklin, Swathi Chakravarthy, Omid Khakshoor, Blake Langdon, Meng Taing, Adolfo Vargas
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Publication number: 20220348604Abstract: The present disclosure provides 3? protected nucleotides, including those 3? protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3? protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.Type: ApplicationFiled: July 6, 2022Publication date: November 3, 2022Inventors: Frank Bergmann, Peter Crisalli, Dieter Hiendl, Omid Khakshoor, Meng Taing
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Patent number: 11440933Abstract: The present disclosure provides 3? protected nucleotides, including those 3? protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3? protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.Type: GrantFiled: December 16, 2019Date of Patent: September 13, 2022Assignee: ROCHE SEQUENCING SOLUTIONS, INC.Inventors: Frank Bergmann, Peter Crisalli, Dieter Hiendl, Omid Khakshoor, Meng Taing
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Publication number: 20220041642Abstract: The present disclosure provides 3? protected nucleotides, including those 3? protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3? protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.Type: ApplicationFiled: December 16, 2019Publication date: February 10, 2022Inventors: Frank Bergmann, Peter Crisalli, Dieter Hiendl, Omid Khakshoor, Meng Taing
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Publication number: 20210292832Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.Type: ApplicationFiled: February 22, 2021Publication date: September 23, 2021Applicant: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Meng Taing, Aruna Ayer, Peter J. Crisalli
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Patent number: 10975432Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.Type: GrantFiled: March 18, 2020Date of Patent: April 13, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Meng Taing, Aruna Ayer, Peter J. Crisalli
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Publication number: 20200385803Abstract: The disclosure relates to tagged nucleoside compounds comprising a nucleotide polyphosphate covalently attached to a tag, wherein the compound is a polymerase substrate and the polymer moiety is capable of entering a nanopore linked to the polymerase and thereby altering the flow of ions through the nanopore. The disclosure also provides methods for preparing the tagged nucleoside compounds and for their use as tags in nanopore-based nucleic acid detection and sequencing.Type: ApplicationFiled: August 25, 2020Publication date: December 10, 2020Inventors: Frank Bergmann, Peter J. Crisalli, Sara Kelley, Omid Khakshoor, Hannes Kuchelmeister, Meng Taing
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Publication number: 20200263248Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.Type: ApplicationFiled: March 18, 2020Publication date: August 20, 2020Applicant: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Meng Taing, Aruna Ayer, Peter J. Crisalli
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Patent number: 10655174Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.Type: GrantFiled: May 24, 2017Date of Patent: May 19, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Meng Taing, Aruna Ayer, Peter J. Crisalli
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Publication number: 20190241952Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.Type: ApplicationFiled: November 26, 2018Publication date: August 8, 2019Inventors: Aruna Ayer, Peter J. Crisalli, Dmitriy Gremyachinskiy, Meng Taing
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Publication number: 20170342485Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.Type: ApplicationFiled: May 24, 2017Publication date: November 30, 2017Applicant: Roche Sequencing Solutions, Inc.Inventors: Dmitriy Gremyachinskiy, Meng Taing, Aruna Ayer, Peter Crisalli
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Patent number: 8173198Abstract: Intermediates and methods for forming activated metal complexes bound to surfaces on oxide layers, immobilizing beads to the modified surface and articles produced thereby are described. Hydroxyl groups on the oxide surfaces are reacted with a metal reagent complex of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a methoxyethanol, a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a plurality of functional groups that are reactive with or that form complexes with Y.Type: GrantFiled: July 23, 2009Date of Patent: May 8, 2012Assignee: Life Technologies CorporationInventors: Steven M. Menchen, Christina E. Inman, Meng Taing, George A. Fry
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Publication number: 20110159305Abstract: Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.Type: ApplicationFiled: March 10, 2011Publication date: June 30, 2011Applicant: APPLIED BIOSYSTEMS, LLCInventors: Steven M. MENCHEN, Christina E. Inman, Meng Taing, Khai Luong, Handong Li
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Patent number: 7928038Abstract: Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.Type: GrantFiled: November 21, 2007Date of Patent: April 19, 2011Assignee: Applied Biosystems, LLCInventors: Steven M. Menchen, Christina E. Inman, Meng Taing, Khai Luong, Handong Li
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Publication number: 20100304381Abstract: Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.Type: ApplicationFiled: February 5, 2010Publication date: December 2, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Meng Taing, Shaheer H. Khan, Steven M. Menchen, Barnett B. Rosenblum
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Publication number: 20100086927Abstract: Intermediates and methods for forming activated metal complexes bound to surfaces on oxide layers, immobilizing beads to the modified surface and articles produced thereby are described. Hydroxyl groups on the oxide surfaces are reacted with a metal reagent complex of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a methoxyethanol, a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a plurality of functional groups that are reactive with or that form complexes with Y.Type: ApplicationFiled: July 23, 2009Publication date: April 8, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Steven M. Menchen, Christina E. Inman, Meng Taing, George A. Fry
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Publication number: 20090092986Abstract: Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.Type: ApplicationFiled: September 29, 2008Publication date: April 9, 2009Applicant: APPLIED BIOSYSTEMS INC.Inventors: Meng TAING, Shaheer H. KHAN, Steven M. MENCHEN, Barnett B. ROSENBLUM