Abstract: A storage system for automatic replenishment includes: a control server and a first robot. The control server is configured to, in a case where it is determined that an item in a picking zone needs to be replenished, generate a replenishment task for the item that needs to be replenished and send a first transporting instruction to the first robot according to the replenishment task; and the first robot is configured to, in response to the first transporting instruction, carry an item associated with the replenishment task from a stocking zone to the picking zone as indicated by the first transporting instruction to allow the item to be replenished into an item container in the picking zone.

Abstract: Provided are a preparation method and system for a recombinant adeno-associated virus (rAAV) and a recombinant bacmid. The method comprises: first reconstructing a recombinant bacmid containing a recombinant baculovirus genome that produces essential functional elements for an rAAV, at least one of the essential functional elements being inserted into the N-terminal or C-terminal of a locus of an essential gene of the recombinant baculovirus genome; and then transfecting the obtained recombinant bacmid containing the recombinant baculovirus genome that produces the rAAV into a host cell line for culturing to prepare an rAAV. Compared with recombinant baculoviruses obtained by conventional Tn7 recombinant preparations of recombinant bacmid, the recombinant baculovirus obtained by inserting a core element containing Cap, Rep and ITR into two sides of a baculovirus essential gene has a more stable rAAV serial passage production level in a cell and has a higher rAAV yield.

Abstract: An inventory region integrating goods preparation and picking, and inventory management system and method. The inventory region comprises: a plurality of high-level shelves and a plurality of picking shelves, wherein any high-level shelf of the plurality of high-level shelves comprises a shelf board, and the shelf board partitions the high-level shelf into at least two levels; and a picking shelf is placed at a bottom level of at least one high-level shelf of the plurality of high-level shelves, and at least one storage container is placed on each of the other floors except for the bottom level where the picking shelf is placed. Inventory articles, which can be picked, are accommodated on each of the picking shelves, and inventory articles for replenishing the picking shelves are accommodated in the storage container.

Abstract: Described herein are compositions and methods for use in targeting neuropilin 2 (NRP2) in lethal prostate cancer, e.g., in metastatic castration-resistant prostate cancer (mCRPC) or neuroendocrine prostate cancer (NEPC).

Abstract: A carrying device includes a bottom plate and a support layer configured to bear an object. The support layer comprises a plurality of protrusions arranged on a first side of the bottom plate and protruding from the first side of the bottom plate along a height direction. The plurality of protrusions are arranged at intervals along a first direction, so that shelf teeth of a warehousing device are configured to enter the support layer, and the first direction is perpendicular to the height direction.

Abstract: Discloses are a method for preparing fluff pulp from bamboos, and the fluff pulp prepared thereby. The method comprises the following steps: treating the bamboos with high-concentration alkali at a relatively mild temperature, after a certain period of treatment, replacing a treatment liquid for separating hemicellulose with a weak black liquor; Kraft cooking the treated bamboos to obtain unbleached bamboo pulp, removing rejects in the unbleached bamboo pulp by using a slotted screen; then removing miscellaneous cells by using a microporous sieve; carrying out two stages of oxygen delignification on the screened pulp; carrying out ECF bleaching treatment of a D0EpD1P process; and finally obtaining the bamboo fluff pulp which has high whiteness, low DCM extract content, low ash content and long fiber length, contains 4.2-4.6 million fibers per gram, and has relatively high bulkiness.

Abstract: A warehousing system (100) is provided. The system is provided with a storage region (100a) and includes a plurality of first shelving units (111) arranged in the storage region (100a), a plurality of travel aisles (120) formed between the first shelving units (111) facing each other, and a first transport passage (131) in communication with the travel aisles (120). The warehousing system (100) further includes second shelving units (112) arranged in an upper-layer space of the first transport passage (131) and located on two sides of extension lines of the travel aisles (120). A height (HL2) of a lowest goods location of each second shelving unit (112) is greater than a height (HL1) of a lowest goods location of each first shelving unit (111).

Abstract: A baculovirus vector and a use thereof in the preparation of a recombinant adeno-associated virus (rAAV) in an insect cell are provided. The baculovirus vector includes an exogenous gene expression cassette and a stable sequence. The stable sequence is located at a site 5 kb or less from the exogenous gene expression cassette, and the stable sequence is a conserved noncoding element (CNE) sequence or a nucleocapsid assembly-essential element (NAE) sequence. When an insect cell is infected with a recombinant baculovirus (rBV) constructed in this way, after multiple continuous passages, production levels of the rBV and the rAAV still remain relatively stable.

Abstract: The present application provides a shelf management method and system, a sorting zone, and a stock sorting system. A shelf management system may include: a mobile robot, configured to transport a shelf; the shelf, configured to be provided with locations for placing commodities and be transportable by the mobile robot; a shelf zone, configured to store the shelf; a station and a station queuing zone, wherein the station is a worker operation position; and the station queuing zone is an area set near the worker operation position, where the mobile robot transporting the shelf queues and waits for a worker's operation; and a server, communicatively connected with the mobile robot and configured to execute a corresponding shelf management method.

Abstract: An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5? end to a 3? end: a first promoter at least used to drive gene transcription of the first ORF; a 5? part of a gene of the first ORF; an intron; and a 3? part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

Abstract: A shelf management method includes predicting whether there is space in the station queue area of a station; if yes, selecting a shelf from among the shelves allocated to the station and have not yet been transported, and controlling a moving robot to transport the selected shelf; after the moving robot is engaged with the selected shelf, re-predicting whether there is space in the station queue areas of all the stations that need the selected shelf; if yes, controlling the moving robot to transport the selected shelf to the station queue area that has been predicted to have space, and when the moving robot has transported the selected shelf to the pre-set area around the station queue area that has been predicted to have space, determining whether there is newly available space in the station queue area; if yes, controlling the moving robot to enter the station queue area.

Abstract: Discloses are a method for preparing fluff pulp from bamboos, and the fluff pulp prepared thereby. The method comprises the following steps: treating the bamboos with high-concentration alkali at a relatively mild temperature, after a certain period of treatment, replacing a treatment liquid for separating hemicellulose with a weak black liquor; Kraft cooking the treated bamboos to obtain unbleached bamboo pulp, removing rejects in the unbleached bamboo pulp by using a slotted screen; then removing miscellaneous cells by using a microporous sieve; carrying out two stages of oxygen delignification on the screened pulp; carrying out ECF bleaching treatment of a D0EpD1P process; and finally obtaining the bamboo fluff pulp which has high whiteness, low DCM extract content, low ash content and long fiber length, contains 4.2-4.6 million fibers per gram, and has relatively high bulkiness.

Abstract: A container taking and conveying assembly includes: a base; a bearing assembly configured to bear a container and defining an accommodating space for accommodating the container; a taking and placing assembly configured to take out the container from a first target position and load the container onto the bearing assembly, or unload the container from the bearing assembly and place the container at a second target position; and a moving assembly configured to drive the taking and placing assembly to move in a first movement trajectory where the moving assembly is configured to drive the taking and placing assembly to move in the accommodating space to load or unload the container, and a second movement trajectory where the moving assembly is configured to drive the taking and placing assembly to leave the accommodating space to allow the container to enter the accommodating space and be borne on the bearing assembly.

Abstract: A warehousing system, includes: a workstation area including at least one sorting station; a goods shelf parking area configured to park a goods shelf; a multifunctional area comprising at least one storage position, and the at least one storage position being configured to store a container transferred from the sorting station or the goods shelf; and a container loading and unloading device configured to transfer the container among the workstation area, the goods shelf parking area, and the multifunctional area.

Abstract: The disclosure discloses an expression cassette for expressing a gene including overlapping open reading frames in an insect cell and an application thereof. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; the overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a native or engineered intron with splicing activity in the insect cell, the intron includes ATG or the intron is located between any two adjacent nucleotides in ATG. A recombinant adeno-associated virus vector including the expression cassette of the disclosure regulates relative expressions of VP1, VP2, and VP3 proteins, and relative expressions of Rep78 and Rep52 proteins by using a designed intron sequence and through an intron splicing function for large-scale production of rAAV.

Abstract: Disclosed are a robot control system and method. The robot control system includes a storage region, a lifting machine, a control device, and a self-driven robot. The storage region includes a loft having at least two storeys and is configured to store a container, and there is provided a passage on the floor of each of the at least two storeys of the loft for the self-driven robot to move through. The lifting machine is configured to transport the self-driven robot or the container to a target storey corresponding to a transportation task. The control device is configured to assign the transportation task to the self-driven robot and plan a travel route on the target storey for the self-driven robot according to the transportation task, and dispatch the self-driven robot to travel according to the travel route to perform the transportation task.

Abstract: Systems and methods herein describe a network system for federated searching. The systems and methods receive a search query, transmit the search query to search providers, receive search results corresponding to the search query, extract metadata from each search result, generate matched groupings comprising a first search results that have matching metadata, generate a ranked list of the matched groupings and a plurality of relevance scores, identify a second subset of search results based on the ranked list of matched groupings, and cause display of the second subset of search results on a graphical user interface of a computing device.

Abstract: The disclosure belongs to the field of genetic engineering and discloses an expression cassette of a gene including overlapping open reading frames and an application thereof in an insect cell. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a first intron and a second intron with splicing activity in the insect cell, the first intron and the second intron share a 5? part or a 3? part, and the artificially constructed sequence does not include a translation start codon; during a post-transcriptional processing process, through an alternative splicing function of the first intron and the second intron, a translation start codon AUG can be formed.

Abstract: Systems and methods herein describe a network system for federated searching. The systems and methods receive a search query, transmit the search query to search providers, receive search results corresponding to the search query, extract metadata from each search result, generate matched groupings comprising a first search results that have matching metadata, generate a ranked list of the matched groupings and a plurality of relevance scores, identify a second subset of search results based on the ranked list of matched groupings, and cause display of the second subset of search results on a graphical user interface of a computing device.

Abstract: Provided are a preparation method and system for a recombinant adeno-associated virus (rAAV) and a recombinant bacmid. The method comprises: first reconstructing a recombinant bacmid containing a recombinant baculovirus genome that produces essential functional elements for an rAAV, at least one of the essential functional elements being inserted into the N-terminal or C-terminal of a locus of an essential gene of the recombinant baculovirus genome; and then transfecting the obtained recombinant bacmid containing the recombinant baculovirus genome that produces the rAAV into a host cell line for culturing to prepare an rAAV. Compared with recombinant baculoviruses obtained by conventional Tn7 recombinant preparations of recombinant bacmid, the recombinant baculovirus obtained by inserting a core element containing Cap, Rep and ITR into two sides of a baculovirus essential gene has a more stable rAAV serial passage production level in a cell and has a higher rAAV yield.