Abstract: A hybridoma cell line secreting an ActA monoclonal antibody and a use thereof. The hybridoma cell line was deposited on Jul. 8, 2021, at the China Center for Type Culture Collection (CCTCC) with the depository accession number of CCTCC NO: C2021174. The present invention also discloses the ActA monoclonal antibody secreted by this hybridoma cell line or its progeny cell line, a detection kit containing this ActA monoclonal antibody, immunomagnetic beads, the preparation method and use of immunomagnetic beads, and competitive ELISA and indirect ELISA detection methods. The ActA monoclonal antibody of the present invention has the advantages of high titer, good specificity, and strong affinity with the natural antigen. The Listeria competitive ELISA detection kit and immunomagnetic beads developed based on this antibody have high sensitivity and good stability, effectively monitoring the level of ActA antibodies in clinical serum samples, and can be used for labeling Listeria monocytogenes.

Abstract: The present disclosure relates to a method for preparing an esterified alkane. The method includes mixing a gaseous alkane with oxygen or air to obtain a mixed gas; adding a chlorine-containing catalyst and/or a nitrogen-containing catalyst to a light-transmission reaction vessel; then adding the mixed gas, an acid and a solvent in sequence to carry out a reaction under ambient pressure and an illumination condition; and then conducting analysis to obtain an NMR yield, followed by extraction, drying, filtration, distillation under reduced pressure and separation by column chromatography to obtain an esterified alkane. The present disclosure has the advantages that the reaction can be carried out under the conditions of ambient temperature and pressure with a cheap and safe chlorine-containing compound and a nitrogen-containing compound as a catalyst and air as an oxidant, and the method has energy-saving and economic effects, convenient and safe operation and environmental friendliness.