Abstract: An oligosaccharide vaccine for specific prevention of fungal infections and a method for preparing the same are provided. The vaccine is formed by conjugating a sulfhydrylated protein with oligosaccharide. The sulfhydrylated protein is formed by introducing a sulfhydryl group (—SH) to a carrier protein containing a primary amino group (—NH2), and then is conjugated with the oligosaccharide to form the oligosaccharide vaccine under the binding action of a bridging agent. The carrier protein is a non-humanized protein, and the oligosaccharide is a chitosan oligosaccharide mixture and/or chitin oligosaccharide mixture. The vaccine has strong immunogenicity, can activate Th17 cell immunity, and can recognize and protect infections caused by fungi.