Abstract: Various techniques for detecting obfuscated web skimmers based on encoding and hooking are disclosed. In some embodiments, a system/process/computer program product for detecting obfuscated web skimmers based on encoding and hooking includes receiving a sample; performing static analysis on the sample using encoded web skimmer detection rules (e.g., encoded using a plurality of encoding methods); performing dynamic analysis on the sample using hooking; and detecting an obfuscated web skimmer based on results of the static analysis using the encoded web skimmer detection rules and/or the dynamic analysis using hooking.

Abstract: The present disclosure relates to a microchannel-based method for obtaining the mechanical modulus of a cell, a microchannel-based jet stream control method, an apparatus, an electronic device, and a storage medium. The microchannel-based jet stream control method includes: obtaining the mechanical modulus of a cell before the cell enters a jet stream region of a microchannel; and adjusting the intensity of a jet stream originated from a jet stream apparatus and used to perforate the cell based on the mechanical modulus of the cell, so that the cell membrane tension of the cell is between a perforation threshold and a rupture threshold. According to the technical solution of the present disclosure, the cell transduction rate, the cell throughput, and the cell survival rate can be improved.

Abstract: The method may include: determining a third frequency-domain data stream, which includes a first frequency-domain data stream and a second frequency-domain data stream, a length of the third frequency-domain data stream is 2N, lengths of both the first frequency-domain data stream and the second frequency-domain data stream are N, data in the first frequency-domain data stream and data in the second frequency-domain data stream are separately mapped to different locations in the third frequency-domain data stream, the first frequency-domain data stream is obtained by performing zero adding processing and Fourier transform processing on a first source data stream, the second frequency-domain data stream is obtained by performing cyclic extension processing on a second source data stream; converting the third frequency-domain data stream into time-domain data and sending the time-domain data to a base station.

Abstract: The present disclosure discloses a strain producing D-allulose 3-epimerase and application thereof, and belongs to the technical field of bioengineering. The present disclosure provides a method for improving the expression of D-allulose 3-epimerase by screening promoters and optimizing RBS thereof. The recombinant Bacillus subtilis constructed using thevectors pP43NMK-hag and pP43NMK-hag-RBS4 provided by the present disclosure improves the enzyme activity of a target gene D-allulose 3-epimerase, and theenzyme activities in shake flasks upon transformation are 1.30 times and 1.69 times that of an original vector. The present disclosure further provides a non-antibiotic resistance vector and a non-antibiotic resistance recombinant B. subtilis strain. Using the non-antibiotic resistance strain B. subtilis 1A751-dal-/pP43NMK-hag-RBS4-dpe-dal provided by the present disclosure, the highest fermentation enzyme activity in a shake flask is 24.72 U/mL, and the enzyme activity in a fermenter is 714.8 U/mL.

Abstract: The present disclosure discloses a strain producing D-allulose 3-epimerase and application thereof, and belongs to the technical field of bioengineering. The present disclosure provides a method for improving the expression of D-allulose 3-epimerase by screening promoters and optimizing RBS thereof. The recombinant Bacillus subtilis constructed using thevectors pP43NMK-hag and pP43NMK-hag-RBS4 provided by the present disclosure improves the enzyme activity of a target gene D-allulose 3-epimerase, and theenzyme activities in shake flasks upon transformation are 1.30 times and 1.69 times that of an original vector. The present disclosure further provides a non-antibiotic resistance vector and a non-antibiotic resistance recombinant B. subtilis strain. Using the non-antibiotic resistance strain B. subtilis 1A751-dal-/pP43NMK-hag-RBS4-dpe-dal provided by the present disclosure, the highest fermentation enzyme activity in a shake flask is 24.72 U/mL, and the enzyme activity in a fermenter is 714.8 U/mL.