Abstract: The invention discloses a device for producing biogas with high methane content by utilizing livestock and poultry feces, wherein the interior of a tank body of a biogas fermentation tank is divided by a baffle, so as to form a main reaction chamber and an auxiliary reaction chamber which are communicated in upper portions, so that a reactant flows into the auxiliary reaction chamber only after entering the main reaction chamber via a relatively low feeding hole and then reaching a high position of a liquid level, and extension of fermentation time is realized, meanwhile, scales formed at the top of fermentation broth flow into the auxiliary reaction chamber along with liquid, so that the interior of the main reaction chamber keeps a liquid state all the time, and sealing and reduction of quantity of anaerobic bacteria are avoided.

Abstract: The invention discloses a device for producing biogas with high methane content by utilizing livestock and poultry feces, wherein the interior of a tank body of a biogas fermentation tank is divided by a baffle, so as to form a main reaction chamber and an auxiliary reaction chamber which are communicated in upper portions, so that a reactant flows into the auxiliary reaction chamber only after entering the main reaction chamber via a relatively low feeding hole and then reaching a high position of a liquid level, and extension of fermentation time is realized, meanwhile, scales formed at the top of fermentation broth flow into the auxiliary reaction chamber along with liquid, so that the interior of the main reaction chamber keeps a liquid state all the time, and sealing and reduction of quantity of anaerobic bacteria are avoided.

Abstract: The present invention is a method of shoot apical meristem transformation for dicot plant via sufficient and micro wounding (SMW). The technical process includes: germinate the seeds in Petri dish or in the nutrient matrix which can be transplanted with the seedling; expose the shoot apical meristem by removing one cotyledon away; make sufficient and micro wounding transformation treatment in vivo to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 ?m in diameter for each one and 0.

Abstract: The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 ?m in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant.