Abstract: High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

Abstract: A drug delivery device comprising a housing body; a piston rod configured to rotate in a rotational direction during dose dispensing under a driving force; and a threaded insert, wherein the piston rod is in threaded engagement with the threaded insert such that during the dose dispensing. the piston rod displaces axially in a distal direction relative to the threaded insert for displacement of a cartridge hung. The threaded insert is movable relative to a stop provided in the housing body and a spring is provided to bias the threaded insert against the stop so that the rotation of the piston rod causes the threaded insert to move away from the stop in response to reaction forces, induced by the cartridge bung into the threaded insert, acting on the piston rod in a proximal direction when the reaction forces exceed a biasing force of the spring.

Abstract: A sensor device removably attachable to a drug delivery device includes a sensing arrangement arranged within the sensor device and circuitry configured to process signals provided by the sensing arrangement. The sensing arrangement is configured such that, when the sensor device is attached to the drug delivery device, the sensing arrangement is operable to read encoded information present on a rotatable component internal to the drug delivery device and that is able to be sensed through a window or aperture of the drug delivery device, and is operable to sense through the window or aperture at least a part of a drive mechanism of the drug delivery device. The circuitry is configured to determine information relating to a set dose, and to determine information relating to whether the drug delivery device is in a dose setting mode or whether the device is in a dose delivery mode.

Abstract: A drug delivery device, comprising: a housing, a plunger arranged within the housing and movable along the longitudinal axis and adapted to push a piston within a container in a distal direction, the plunger being prevented from rotating and comprising an external thread, a drive nut arranged within the housing and comprising an internal thread engaging the thread of the plunger, an axial bearing arranged between the drive nut and the housing to limit axial movement of the drive nut relative to the housing, a mechanism configured to exert a force on the drive nut in the distal direction through a rotatable component coupled to the drive nut for joint rotation and at least one non-rotatable component, wherein a bearing is arranged between the at least one rotatable component and the at least one non-rotatable component, the bearing having a smaller diameter than the axial bearing.

Abstract: Technologies for using an inferred relationship between data sets to computationally align the data sets and generate actionable output include obtaining, from an online system, a set of member records; inferring a job category based at least partly on job function data extracted from the set of member records; obtaining, from the management system, a set of job records that correspond to the inferred job category; calculating an aggregate alignment score by comparing member skills data extracted from the set of member records to job skills data extracted from the set of job records, where the aggregate alignment score is a measure of a mismatch between the member skills data for the set of member records and the job skills data for the set of job records; based on the aggregate alignment score, identifying a skill that is actionable to address the mismatch between the member skills data and the job skills data; generating actionable output that replaces, modifies, or supplements first course recommendation d

Abstract: A sensor device removably attachable to a drug delivery device includes a sensing arrangement arranged within the sensor device and circuitry configured to process signals provided by the sensing arrangement. The sensing arrangement is configured such that, when the sensor device is attached to the drug delivery device, the sensing arrangement is operable to read encoded information present on a rotatable component internal to the drug delivery device and that is able to be sensed through a window or aperture of the drug delivery device, and is operable to sense through the window or aperture at least a part of a drive mechanism of the drug delivery device. The circuitry is configured to determine information relating to a set dose, and to determine information relating to whether the drug delivery device is in a dose setting mode or whether the device is in a dose delivery mode.

Abstract: A set including a pump dispenser for discharging pharmaceutical or cosmetic liquids and an external evaluation unit, which is designed to be exchangeable so that, after the pump dispenser has been emptied, the evaluation unit can be attached to a new pump dispenser. The evaluation unit can be attached to the discharging head of the pump dispenser such that the evaluation unit detects an actuation button and can be partially displaced with same. The evaluation unit can alternatively have a receiving container which is open on at least one side and into which the pump dispenser can be inserted and the force application of which is detected on actuation.

Abstract: The present disclosure is generally directed to a drug delivery device for selecting and dispensing a number of user variable doses of a medicament. The device comprises a housing and at least two separate component parts, wherein for example at least one of these component parts performs a movement relative to the housing during dose setting and for example at least one of these component parts performs a movement relative to the housing during dose dispensing, and wherein the relative movement of the at least two separate component parts with respect to each other during dose setting differs from the relative movement of the at least two separate component parts with respect to each other during dose dispensing.

Abstract: The present disclosure is generally directed to a clicker arrangement for use in a drug delivery device, i.e. a handheld injection device for selecting and dispensing a number of user variable doses of a medicament. The clicker arrangement comprises a first element rotatable at least in a first direction and a second, non-rotatable element. The first element is axially displaceable relative to the second element between a first position and a second position. One of the elements comprises a clicker arm and the other comprises a protrusion wherein upon rotation of the first element in the first direction relative to the second element the clicker arm is elastically deflectable in a radial direction by the protrusion and relaxable in the opposite radial direction upon disengagement with the protrusion thereby generating an audible and/or tactile feedback signal.

Abstract: The disclosure relates to a drug delivery device for expelling a pre-determined or pre-settable amount of a liquid medicament formulation which improves the stalling behavior and indicates the stalling state to the user. The device comprises: a medicament reservoir attached to a housing and an expelling mechanism configured for acting against the medicament reservoir in order to expel a portion of the liquid medicament formulation therefrom, the expelling mechanism comprising an arrangement of a threaded nut in a fixed axial relation to the housing and a lead screw in threaded engagement with the threaded nut, the threaded nut and the lead screw being rotatable relative to each other by a rotational input interface, a mechanical energy reservoir (for storing energy, the stored energy being releasable from the energy reservoir by a rotational interface, a drive train having upstream and downstream interfaces; and a trigger movable relative to the housing.

Abstract: The present disclosure relates to a drug delivery device comprising a dose setting member for setting user variable doses of a medicament and a display for indicating the dose set by the dose setting member. The display comprises a number wheel, which is rotationally coupled to the dose setting member and is provided with a series of markings on its outer circumference, and at least one prism deviating the image of the markings of the number wheel, e.g. by 90°.

Abstract: The present disclosure relates to a ratchet mechanism and to an injection device for expelling of a number of preset or user-selectable doses of a medicament. The ratchet mechanism comprises a housing and a ratchet member that is circularly shaped and rotationally supported relative to the housing. The ratchet member comprises a ratchet surface, a plurality of ratchet features on the ratchet surface, and one or more intermediate ratchet sections on the ratchet surface extending between the plurality of ratchet features. The ratchet mechanism further comprises a counter ratchet member that is circularly shaped, coaxially arranged relative to the ratchet member, rotatable relative to the ratchet member at least along a first sense of rotation, and rotationally supported relative to the housing. The counter ratchet member comprises a counter ratchet surface, a plurality of counter ratchet features on the counter ratchet surface, and one or more intermediate counter ratchet sections.

Abstract: The present disclosure is generally directed to a drive mechanism for use in a drug delivery device having a cylindrical cartridge, i.e. a handheld injection device for selecting and dispensing a number of user variable doses of a medicament. The drive mechanism includes a base element, a toothed piston rod, which is guided within and movable relative to the base element, and a drive gear having a pinion, which is rotatably held in the base element and in meshed engagement with the toothed piston rod. The toothed piston rod includes multiple rigid rod pieces which are connected by hinges. The rigid rod pieces comprise a flat plate provided with a straight toothed rack.

Abstract: A drive train for a wind-up injection device, comprises a torsional energy storage adapted to be loaded or unloaded by a rotatable element, a rotatable user handle coupled with a button, a rotationally drivable expelling mechanism, and a clutch element coupled with the torsional energy storage via the rotatable element and comprising a ratchet for maintaining the rotatable element at one of a number of discrete angular positions, wherein via the rotatable element, the clutch element is adapted to transmit a torque from the user handle to the torsional energy storage or from the torsional energy storage to the expelling mechanism, and wherein the ratchet is switchable from one position to an adjacent position by a torque transmitted from the user handle to the torsional energy storage, and wherein the drive train is adapted to dampen torque peaks by a flexible impact portion in the area of the clutch element.

Abstract: The present disclosure relates to an injection device for expelling of a number of preset or user-selectable doses of a medicament, the injection device comprising: —an elongated housing (10; 310; 410) extending along a longitudinal axis (1), —an expelling mechanism (2) comprising a trigger (70) and a piston rod (30), —a dose setting mechanism (3) comprising a rotatable member (260; 360; 460; 560) and a counterpart member (240; 340; 440; 540), wherein the counterpart member and the rotatable member comprise a first mechanical code (242; 342; 442; 542) and a second mechanical code (262; 362; 462; 562), respectively, —wherein for setting of a dose the rotatable member (260; 360; 460; 560) is rotatable relative to the housing (10; 310; 410) and relative to the counterpart member (240; 340; 440; 40) within a range of numerous rotational states, and wherein during setting of the dose the rotatable member (260; 360; 460; 560) is constrained along the longitudinal axis (1) relative to the housing (10; 310; 410) or r

Abstract: A drive train (T) for a wind-up injection device (1) for injecting a liquid drug comprises a torsional energy storage (9) adapted to be loaded or unloaded by a rotatable element (6), a rotatable user handle (8), a rotationally driveable expelling mechanism (5) adapted to expel the liquid drug and a clutch element (12) coupled with the torsional energy storage (9) via the rotatable element (6) and comprising a ratchet (12.4) for maintaining the rotatable element (6) at one of a number of discrete angular positions against the torque of the torsional energy storage (9). The clutch element (12) is adapted to transmit a torque from the user handle (8) via the rotatable element (6) to the torsional energy storage (9) or alternatively from the torsional energy storage (9) via the rotatable element (6) to the expelling mechanism (5), wherein the ratchet (12.4) is switchable from one position to an adjacent position by a torque transmitted from the user handle (8) to the torsional energy storage (9).

Abstract: A button (70, 270, 370, 470) for a drug delivery device with a reduced operating loudness comprises a plate-like button body forming a touch surface (70b, 270b, 370b, 470b), wherein the plate-like button body is coupled by an axial supporting member (75, 275, 375, 475) to a noise-generating interface of the drug delivery device and movable into a longitudinal direction of the drug delivery device, wherein the plate-like button body comprises a material composite with at least one first component (271, 272, 371, 372, 373, 374, 471) consisting of a first material and at least one second component (278, 378, 478) consisting of a second material different from the first material, wherein the at least one first component (271, 272, 371, 372, 373, 374, 47) and the at least one second component (278, 378, 478) are coupled via at least one coupling plane (270d, 270e, 370d, 470d) that is at least sectionwise slanted or perpendicular to the longitudinal direction.

Abstract: A drug delivery device for selecting and dispensing a number of user variable doses of a medicament includes a housing having an opening, a dose selector operable to set a dose by rotation relative to the housing, a release member controlling movement of a piston in a dispensing direction, and a display comprising a units number wheel and a tens number wheel which are coupled to each other such that a continuous rotation of the units number wheel is translated into an intermittent rotary motion of the tens number wheel. The release member rotates, during dose dispensing, an angle corresponding to the dose set by the dose selector, thereby allowing a piston to move in a dispensing direction.

Abstract: This disclosure refers to a dosing mechanism for use in a drug delivery device implementing a dialing mode and a dispensing mode, wherein the dosing mechanism comprises a dose button, a housing and a determination unit. The dose button is activatable by the user for dose dispense, causing displacement of an indicating element connected to the housing from a first position to a second position. The determination unit is adapted such that it detects the indicating element in its second position and thereby determines that the dosing mechanism is in the dispensing mode. With some embodiments, it is possible to distinguish between the dialing mode and the dispensing mode. Some embodiments further refer to a drug delivery device comprising the above dosing mechanism.

Abstract: A dose setting mechanism comprising a first element and a second element; the first element being moveable with respect to the second element; the first element and the second element being coupled by a ratchet mechanism allowing motion of the first element with respect to the second element from a first position to a second position only in a first direction while preventing motion in a second direction that is opposite to the first direction; once passing the second position, the first element being moveable with respect to the second element in the first direction and in the second direction.