Abstract: A system for detecting and analyzing patient sample quality and/or analytes, while the sample is in the metering tip used to aspirate the sample liquid from an original patient sample container, and also to dispense the liquid onto a slide test element. Spectrophotometric analysis may be done on the sample liquid while it is still in the tip which has been converted into a cuvette. One technique for such analysis is by scanning the cuvette for transmittance in a light-tight enclosure. Near-infrared and adjacent visible radiation may be used, and the absorption spectra of the liquid detected and analyzed. A possible aspect of the present invention relates to enhancing throughput of an analyzer by conducting a sample quality measurement in a process that is parallel to the main analyzer timing cycle.

Abstract: A method for removing an undesired component from a bound desired component in an analysis that includes the steps of: (a) providing a container having a desired component bound thereto and an undesired component; (b) dispensing a wash fluid in the container at a first level sufficient to contact at least a portion of the bound desired component; (c) oscillating the level of the wash fluid in the container; and (d) removing at least a portion of the wash fluid from the container. Another aspect of the invention provides a method for washing an analyte bound to the walls of a surface coated container of an analyzer that includes: (a) providing a surface coated container having an analyte bound thereto; (b) dispensing a wash fluid in the container at a first level sufficient to contact at least a portion of the analyte bound thereto; (c) oscillating the level of the wash fluid in the container; and (d) removing the wash fluid from the surface coated container.

Abstract: A metering tip for use in a clinical analytical apparatus includes a tapered body having at least one interior stepped areas. Each of the stepped areas include a sharp diametrical edge for latching a fluid meniscus being dispensed from the tip and for reducing fluid oscillation during metering.

Abstract: Apparatus and a method for mixing a liquid within a disposable aspirating probe tip so that most of the liquid is forced to move past a transition zone between two different inside diameters to cause rotational mixing. The apparatus and method can be used to provide agglutination of blood, which in turn can be used for blood typing. The probe tip can comprise a single integral piece, or two separate portions. The transition zone can comprise a sharp demarcation between inside diameters, or a smooth one.

Abstract: A method for improving the fluid dispense rate in a metering system utilizing speed includes the steps of modifying the fluid flow rate profile during a portion thereof so as substantially increase or decrease the speed of the motor during certain portions of a metering cycle in order to improve the efficiency of the metering system. For a variable speed pump producing a sinusoidal speed profile, the pump motor speed can be increased to increase the dispense velocity during the beginning and end of the dispense phase to reduce perfusion and improve metered volume precision or a motor speed profile can be applied which is inverted a relative to that of the variable speed pump to produce a constant velocity flow. Alternately or in addition to the above, the home position of the variable speed pump can be shifted to produce fluid velocity during the entirety of a dispense portion of a metering system cycle.

Abstract: Apparatus and a method for mixing a liquid within a disposable aspirating probe tip so that most of the liquid is forced to move past a transition zone between two different inside diameters to cause rotational mixing. The apparatus and method can be used to provide agglutination of blood, which in turn can be used for blood typing. The probe tip can comprise a single integral piece, or two separate portions.

Abstract: Apparatus and a method for mixing a liquid within a disposable aspirating probe tip so that most of the liquid is forced to move past a transition zone between two different inside diameters to cause rotational mixing. The apparatus and method can be used to provide agglutination of blood, which in turn can be used for blood typing. The probe tip can comprise a single integral piece, or two separate portions.

Abstract: A method of dispensing wash solution to separate free from bound labels in a slide immunorate assay. The method comprises dispensing wash first as separate drops spaced to allow complete absorption prior to the next drop, followed by a time at which the rate of dispensed wash exceeds the rate of fluid uptake of the slide, to form a continuous stream. The first phase of this wash method provides a more complete separation of bound from free under the dispense tip than occurs if only a continuous stream is used throughout.

Abstract: A holder of slide test elements for use in an analyzer at a sample-dispensing station, the holder comprising two opposing holding surfaces for holding generally horizontally a slide test element at opposite side edges of the test element, and a tower extending above the holding surfaces for generally centering a dispensing tip above a held slide test element. The holder is improved in that one of the holding surfaces of the holder extends at least sufficiently for underneath a slide test element held by the holding surfaces to be disposed under an approximate center of the held test element, and includes a raised reference surface located under, and disposed for abutting against, an under-surface of the held slide test element, so that a held slide test element is prevented by the raised reference surface from warping downwardly away from a dispensing tip in the tower.

Abstract: A centrifuge and method of operating it, wherein a patient sample tube is first spun while non-aligned with the centrifugal force to make use of the Boycott effect, and then while aligned with the centrifugal force to allow any gel present between the separated phases to properly seal. A latch is used to hold it in the non-aligned position until the latch is opened.

Abstract: A method for reducing interferent bias such as hemoglobin bias in immunoassays using dried slide test elements featuring peroxidase and leuco dye as the labeling mechanism.

Abstract: A method for verifying the effectiveness of a washing operation on a clinical dry chemistry slide element in which wash liquid is dispensed onto the surface of the slide element and then detection devices are used to verify the effectiveness of the wash by making an initial detection at an on center site followed by making a second detection at a second and off-center radial position. A comparison of the two readings provides verification of the wash. In addition, rate measurements can be made at each of the read positions to measure the effectiveness of the wash and to verify sample application for immunological assays.

Abstract: A method and related apparatus for the dispensing of a biological liquid, such as whole blood, to a diagnostic test element is described, in which a quantity of patient sample is preferably added to a container having contained filters for separating and supporting a quantity of the sample. The container has an exterior surface wettable with the filtered sample, a cover to prevent significant evaporation of the wetted filter surface, and a movable transfer element for transferring filtered liquid from the wetted surface and delivering the liquid to a test element.

Abstract: An analytical element for quantitatively assaying an analyte by measuring reflection density in the range 320 to 360 nm. The element comprises a reagent layer and a registration layer. One of such layers contains a dye which has a low absorbtivity band from 320 to 360 nm and a high absorbtivity band from 640 to 720 nm.

Abstract: There is described a cover for test elements for use in stations of an analyzer that are downstream from the sample-spotting station. The cover is improved in that its undersurface is grooved lengthwise to accommodate a drop of sample protruding from the test element. However, the clearance groove does not extend the full length of the cover, to prevent significant evaporation.

Abstract: There are disclosed polyethylene evaporation covers and a method of analysis that uniquely solve the problem of contamination of CKMB test elements when phosphorous test elements are incubated in the same incubator. The solution is to have the surface of the evaporation cover the contacts the test element, be non-porous polyethylene.

Abstract: There is disclosed apparatus in which an aspirating and dispensing tip is provided with liquid wherein residual liquid is left as a sheath on the exterior of the tip after aspiration. The apparatus is improved to include means exterior of the dispensing tip for separating the sheath into at least two parts without detaching residual liquid physically from the exterior surface, the two parts being separated along the axis of the tip by a dry annular portion of the exterior surface having a suitable width. Such separating means is spaced away from the tip a distance effective to prevent contact of the separating means with either the tip or the liquid of the sheath.

Abstract: Apparatus is disclosed for transferring liquid out of a container covered by a pierceable cap using a storage unit. The apparatus includes means for moving the storage unit into an aspirating position within the capped container, means for aspirating the liquid into the storage unit, means for moving the storage unit away from such aspirating position, and means for dispensing at least a fraction of the liquid out of the storage unit. The apparatus further includes means for holding such container during all movement of the storage unit away from its aspirating position.