Abstract: Disclosed herein is a system that relies upon an assay card employing only capillary action and no outside energy source containing cargo-loaded gated porous nanoparticles, an optional device cartridge, an optional reagent container, and optional software for detection and/or quantitation of at least one target analyte of a liquid sample.

Abstract: The present invention relates to proteome chips comprising arrays having a large proportion of all proteins expressed in a single species. The invention also relates to methods for making proteome chips. The invention also relates to methods for using proteome chips to systematically assay all protein interactions in a species in a high-throughput manner. The present invention also relates to methods for making and purifying eukaryotic proteins in a high-density array format. The invention also relates to methods for making protein arrays by attaching double-tagged fusion proteins to a solid support. The invention also relates to a method for identifying whether a signal is positive.