Abstract: A microscopic confocal fluorescence spectrometer is provided. The microscopic confocal fluorescence spectrometer includes an optical system and an instrument body. By adopting an open microscopic fluorescence light path, a lens body part of an existing spectrometer microscope is omitted, so that the space behind an objective lens is significantly increased. By reasonably arranging a laser, a spectrum detection device and other components, the space on the side of the objective lens is further increased, and the occupied space of the optical system is reduced, so that the overall volume of the instrument is reduced, and the adaptability of the instrument is further improved. In addition, the spectrum detection device is arranged outside the second shell to facilitate the heat dissipation of the spectrum detection device, so that the heat stability and working reliability of the instrument are further improved.