Abstract: Provided are a method for preparing an mRNA-GalNAc targeting molecule, an in vivo delivery system therefor and use thereof. The mRNA-GalNAc targeting molecule comprises an mRNA molecule that is linked to PolyA modified with an N-acetylgalactosamine at 3?-end, wherein a sequence of the mRNA molecule comprises a 5? cap and a target gene sequence. By directly linking the mRNA molecule expressing the target gene to the polyA sequence coupled with GalNAc, an mRNA molecule with GalNAc at 3?-end is synthesized to realize the aim of targeted drug delivery in liver. The method is simpler and more reliable, and solves existing problems in coupling between mRNA and N-acetylgalactosamine.

Abstract: Embodiments of the present application provide a server of a battery swapping station, a charging method and system for a battery, a device and a medium. The method is applied to a server of a battery swapping station, and the method includes: acquiring version information of a first software version after a vehicle mounted with a power battery arrives at the battery swapping station, wherein the first software version is a software version of a first battery management unit of the power battery; controlling, under a condition that the version information of the first software version is lower than version information of a second software version stored in the server, a second battery management unit of the battery swapping station to update the first software version of the first battery management unit to the second software version.

Abstract: Embodiments of the present application provide a vehicle control method, module, and system, a device and a medium. The method includes: acquiring, under a condition that it is determined to install a target battery provided by a battery swapping station for the vehicle, a network address of a first battery management unit of the target battery; requesting to establish a wireless communication connection between a control module and the first battery management unit, based on the network address of the first battery management unit of the target battery; transmitting, under a condition that the wireless communication connection between the control module and the first battery management unit is successfully established, a first prompt signal to a target object, where the first prompt signal is used to prompt the target object to perform a relevant operation for allowing the vehicle to leave a battery swapping area.

Abstract: Battery swapping methods, apparatus, systems, devices and media are provided in the embodiments of the present disclosure. The method includes: receiving a battery swapping request of a vehicle; determining a second battery swapping time and a second swapping electricity amount based on a first battery swapping time and a first swapping electricity amount, in a case where a first charging parameter is adjusted to a second charging parameter to charge an available battery of a battery swapping station; and sending a battery swapping response to a terminal device of the vehicle, wherein the battery swapping response includes the second battery swapping time and the second swapping electricity amount, the second battery swapping time is a recommended battery swapping time provided by the battery swapping station, and the second swapping electricity amount is a recommended swapping electricity amount provided by the battery swapping station.

Abstract: Embodiments of the present application provide a battery swapping method, module, device and medium. The method includes: acquiring a first image captured for a vehicle within a battery swapping area, and acquiring a first location of a battery on the vehicle; determining a second location of the vehicle in the battery swapping area according to the first image; determining, according to the first location and the second location, a target location of the battery in the battery swapping area to control a battery swapping device to move to the target location for battery swapping. According to the embodiments of the present application, battery swapping can be completed in a case where a user parks the vehicle randomly in the battery swapping area. Thus, convenience of the battery swapping process is improved.

Abstract: The embodiments of the present application provide a method, apparatus, device, and medium for electric energy transmission. The method includes: determining a resource value of a unit of electric energy of a grid at a target time; under a condition that the resource value is greater than or equal to a first resource threshold, determining a number of available batteries in a battery swapping station and an ordered number of batteries in the battery swapping station within a target time period; and under a condition that the number of available batteries is greater than a number of reserved batteries, controlling a charging and discharging module to transmit electric energy of a first battery in the battery swapping station to the grid.

Abstract: Provided are a strain ofBeijerinckia fluminensis and an application thereof in arsenic oxidation. The strain is AS-56, and has been deposited in the China Center for Type Culture Collection on 5 Dec. 2019, the deposit number being CCTCC NO: M 20191014. The strain AS-56 of Beijerinckia fluminensis can oxidise As(III) to the less toxic As(V) and can completely oxidize 0.15 g/L of As(III) solution to As(V), greatly reducing arsenic toxicity, and thus having wide prospects for application in environmental remediation.

Abstract: Provided are a method for large-scale synthesis of a long-chain RNA and a method for site-specific modification of the long-chain RNA. The method for large-scale synthesis of a long-chain RNA comprises: designing short RNA fragments and splint DNA fragments; ligating; capping; and removing the splint DNA fragments and other steps. A large number of short RNA fragments and different splint DNA fragments are chemically synthesized, and then the different short RNA fragments are ligated by a biological method so as to form a target long-chain RNA. The product long-chain RNA has a low mutation rate, a plurality of the short RNA fragments can be assembled in a single reaction, and the long-chain RNA can be synthesized at a high throughput so as to fulfill the large-scale production of the long-chain RNA. In addition, by chemical modification of the short RNA fragments, the site-specific modification of the long-chain RNA can be realized.

Abstract: The invention discloses a method for identifying the bacterial species and key functional genes thereof involved in antimony reduction in the soil. After consuming the original substrate by starvation culture, the sole metabolic substrate is added and the sole electron acceptor Sb(V) is provided, so that there is only one dominant electron exchange process in the system. The microorganisms metabolize and oxidize the organic substrate while coupling with the reduction of antimony, so that Sb(V) gets electrons and is reduced to Sb(III). The present invention observes the Sb(V) reduction in an anaerobic culture system of paddy soil under Sb(V) stress, and uses DNA-SIP technology to identify the phylogenic information of microorganisms that can drive the Sb(V) reduction in the culture system.

Abstract: Disclosed herein are an mRNA targeting molecule comprising an N-acetylgalactosamine binding polypeptide and a preparation method therefor. A plasmid vector containing a DNA fragment formed by sequentially connecting a promoter, a target gene, a specific protease cleavage sequence, and a polynulcleotide sequence encoding a GBD capable of binding to N-acetylgalactosamine, is transcribed to obtain an mRNA, which is connected to a DNA-puromycin linker under the action of a T4 ligase. The resulting connection product is subjected to protein translation, followed by cleavage using a specific protease to obtain an mRNA-puromycin-GBD complex, which then binds to a GBD protein sequence under the action of an N-acetylgalactosamine transferase to form an mRNA-puromycin-GBD-GalNAc complex, thereby modifying the mRNA with GalNAc, thus achieving the purpose of precise administration in a process of mRNA drug delivery and increasing the efficacy of the mRNA drug molecule.

Abstract: The present invention provides a mannose-based mRNA targeted delivery system and use thereof. The mRNA can encode one or more target polypeptides and contains at least one mannose modification. The technical solution of the present invention modifies the mRNA molecule with a mannose, so that the mRNA can be directly and efficiently coupled with the mannose, and the targeted delivery of the mRNA is realized without the need for a carrier.

Abstract: The present application discloses a battery management system, a processing device, a battery management method, and a battery management and control system.

Abstract: A method for reconstructing a signal from a subset of the full signal is disclosed. In one embodiment, the method includes receiving a plurality of randomly sampled multichannel radio-frequency or in-phase and quadrature signals acquired from a physical environment including a transmitter, a propagation medium and a receiver; modelling the full signal as a matrix comprising a plurality of vector signals, wherein the full signal is expressed as a matrix product of a first matrix and a second matrix; updating the second matrix based on an objective function to produce an updated second matrix; determining a convergence parameter in dependence upon the evaluated objective function; and modifying the updated second matrix in dependence upon the convergence parameter.

Abstract: In one implementation, a redactable document signature system includes an encoding engine, a reordering engine, and a signature engine. The encoding engine is to access a plurality of subdocuments of a document, to generate a plurality of commitment values from the plurality of subdocuments, and to generate a plurality of dummy values. Each dummy value is indistinguishable from a commitment value. The reordering engine is to define an order of the plurality of commitment values and the plurality of dummy values independent of an order of the subdocuments. The signature engine is to calculate a signature value for the document using the plurality of commitment values and the plurality of dummy values according to the order.

Abstract: In one implementation, a redactable document signature system includes an encoding engine, a reordering engine, and a signature engine. The encoding engine is to access a plurality of subdocuments of a document, to generate a plurality of commitment values from the plurality of subdocuments, and to generate a plurality of dummy values. Each dummy value is indistinguishable from a commitment value. The reordering engine is to define an order of the plurality of commitment values and the plurality of dummy values independent of an order of the subdocuments. The signature engine is to calculate a signature value for the document using the plurality of commitment values and the plurality of dummy values according to the order.

Abstract: In one implementation, a redactable document signature system includes an encoding engine, a reordering engine, and a signature engine. The encoding engine is to access a plurality of subdocuments of a document, to generate a plurality of commitment values from the plurality of subdocuments, and to generate a plurality of dummy values. Each dummy value is indistinguishable from a commitment value. The reordering engine is to define an order of the plurality of commitment values and the plurality of dummy values independent of an order of the subdocuments. The signature engine is to calculate a signature value for the document using the plurality of commitment values and the plurality of dummy values according to the order.

Abstract: Embodiments of the present application provide a beamforming based communications method and apparatus. The method includes: obtaining, by a transmit end, a transmit-end precoding matrix TrBB, a transmit-end intermediate-frequency beamforming matrix TrIF, and a transmit-end radio-frequency-end beamforming matrix TrRF according to feedback information that is from a receive end; acquiring a first data stream, and performing precoding processing on the first data stream according to TrBB, to generate a first analog signal; performing weighting and power amplification processing on the first analog signal according to TrIF, to generate a second analog signal; performing weighting and power amplification processing on the second analog signal according to TrRF, to generate a third analog signal; and determining an antenna array matching the third analog signal, and transmitting the third analog signal to the receive end by using the antenna array matching the third analog signal.

Abstract: The present invention provides a multi-channel array distortion compensation apparatus and method. The apparatus determines, according to a status of a first indication signal, whether to trigger a signal compensation operation; determines, according to a status of a second indication signal, a first adjustment condition is met, compares power of a received signal with a power threshold, and if the power of the received signal is greater than the threshold power, compensates the received signal according to a first adjustment factor; and if the status of the second indication signal does not meet the first adjustment condition, determines the status of the second indication signal meets a second adjustment condition, performs auxiliary compensation according to a second adjustment factor. By compensating signal distortion caused by chip stacking for a received signal, this reduces costs for implementing a multi-channel array system without the need to increase system complexity.

Abstract: The present invention provides a multi-channel array distortion compensation apparatus and method. The apparatus determines, according to a status of a first indication signal, whether to trigger a signal compensation operation; determines, according to a status of a second indication signal, a first adjustment condition is met, compares power of a received signal with a power threshold, and if the power of the received signal is greater than the threshold power, compensates the received signal according to a first adjustment factor; and if the status of the second indication signal does not meet the first adjustment condition, determines the status of the second indication signal meets a second adjustment condition, performs auxiliary compensation according to a second adjustment factor. By compensating signal distortion caused by chip stacking for a received signal, this reduces costs for implementing a multi-channel array system without the need to increase system complexity.