Patents by Inventor Michael A. Dalton

Michael A. Dalton has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
    Publication number: 20030211506
    Abstract: The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI and other nicking enzymes in non-modified strand displacement amplification, is disclosed also.
    Type: Application
    Filed: November 14, 2002
    Publication date: November 13, 2003
    Inventors: Huimin Kong, Lauren Sears Higgins, Michael A Dalton, Rebecca B Kucera, Ira Schildkraut, Geoffrey G Wilson
  • Method for cloning and producing the SwaI restriction endonuclease
    Patent number: 6245545
    Abstract: The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
    Type: Grant
    Filed: April 27, 1999
    Date of Patent: June 12, 2001
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren S. Higgins, Michael A. Dalton
  • Method for cloning and producing the SgrAI restriction endonuclease
    Patent number: 6048731
    Abstract: The present invention relates to the recombinant DNA which encodes the SgrAI restriction endonuclease and the MspI modification methylase, and the production of SgrAI restriction endonuclease from the recombinant DNA.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: April 11, 2000
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren S. Higgins, Michael A. Dalton
  • Method for cloning and producing the DraIII restriction endonuclease
    Patent number: 6048719
    Abstract: The present invention relates to the recombinant DNA which encodes the DraIII restriction endonuclease modification methylase, and the production of DraIII restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 vector which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 vector which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
    Type: Grant
    Filed: January 22, 1999
    Date of Patent: April 11, 2000
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren S. Higgins, Michael A. Dalton
  • Method for cloning and producing the AvaI restriction endonuclease in E. coli and purification of the recombinant AvaI restriction endonuclease
    Patent number: 6004793
    Abstract: A recombinant restriction endonuclease from Anabaena variabilis is provided, as well as the isolated gene which encodes it and methods for the production of the recombinant AvaI restriction endonuclease. An isolated gene encoding a modification methylase from A. variabilis is also provided.
    Type: Grant
    Filed: August 18, 1998
    Date of Patent: December 21, 1999
    Assignee: New England Biolabs, Inc.
    Inventors: Keith D. Lunnen, Michael A. Dalton, Geoffrey G. Wilson, Shuang-yong Xu