Abstract: The present invention provides immunogenic compositions having one or more polysaccharide-protein conjugates in which one or more polysaccharides from Streptococcus pneumoniae bacterial capsules are conjugated to a carrier protein in an aprotic solvent such as dimethylsulfoxide (DMSO). The present invention also provides methods for providing an enhanced immune response to a pneumococcal polysaccharide protein conjugate vaccine comprising administering to a human subject an immunogenic composition comprising polysaccharide-protein conjugates prepared in DMSO conditions.

Abstract: The present disclosure relates generally to detection of contamination of a sample or diagnosis of subject based upon detection or quantification of amino acid sequences in a sample, specifically to the identification and use of molecular biomarkers for Candida albicans biofilm infections.

Abstract: An after-fan system for an engine may comprise an after-fan turbine an electrical generator operationally coupled to the after-fan turbine, and an electric motor electrically coupled to the electrical generator. The electrical generator may be configured to generate an electrical current in response to rotation of the after-fan turbine. The electric motor may be configured to generate torque.

Abstract: A method is described for reconstituting lyophilized mutant diphtheria toxin in dimethyl sulfoxide for use in producing pneumococcal capsular polysaccharide mutant diphtheria toxin conjugates.

Abstract: A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.

Abstract: The present invention provides immunogenic compositions having one or more polysaccharide-protein conjugates in which one or more polysaccharides from Streptococcus pneumoniae bacterial capsules are conjugated to a carrier protein in an aprotic solvent such as dimethylsulfoxide (DMSO). The present invention also provides methods for providing an enhanced immune response to a pneumococcal polysaccharide protein conjugate vaccine comprising administering to a human subject an immunogenic composition comprising polysaccharide-protein conjugates prepared in DMSO conditions.

Abstract: A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized in the form of lyospheres of carrier protein and lyospheres of the one or more polysaccharides. A predetermined amount of carrier protein lyospheres and activated polysaccharide lyospheres are mixed together and the mixture reconstituted in an organic solvent to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.

Abstract: The invention relates to a flywheel unit with a damping device for fastening the flywheel unit and a flywheel energy store. The flywheel unit comprises a rotor, a machine housing enclosing the rotor and with an underside, and a damping device fastened to the underside of the machine housing for fastening the flywheel unit to a suitable foundation, wherein the damping device comprises a lower stand unit for fastening to the foundation, an upper stand unit for fastening to the underside of the machine housing, and a membrane connecting the lower stand unit with the upper stand unit, wherein the upper and lower stand units as well as the membrane are configured in such away, that the membrane can establish a pliable self-supporting connection between the upper and lower stand units.

Abstract: Hybrid electric propulsion systems are described. The systems include a gas turbine engine having low and high speed spools, each spool having a respective compressor and turbine. A mechanical power transmission is configured to at least one of extract power from and supply power to at least one of the low speed spool and the high speed spool, an electric machine is configured to augment rotational power of at least one of the spools, and a controller is operable to determine a rotational speed of the low speed spool, determine a rotational speed of the high speed spool, determine if a predetermined operational zone of operation based on the determined rotational speeds is present, and when a predetermined operational zone is determined, control a power augmentation of at least one spool to limit dwell operation of the gas turbine engine within the predetermined operational zone.

Abstract: A system includes a recirculation line, a mainline flow meter operable to measure a flowrate of fluid flowing through the recirculation line, a mixing chamber, an inlet line coupled between the recirculation line and the mixing chamber, at least one chemical injection port coupled to the inlet line, a dedicated feed pump operably associated with each chemical injection port, and an outlet line coupled between the mixing chamber and the recirculation line. The mixing chamber includes a plurality of mixing zones, a mixing blade assembly that includes at least one blade within each mixing zone, and a motor coupled to the mixing blade assembly and operable to rotate the mixing blade assembly. Each of the dedicated feed pumps is coupled to a separate chemical supply and is operable to pump a chemical to the corresponding chemical injection port for injection into the inlet line.

Abstract: A packaged food product processing machine. The machine comprises a food consumer interface configured to receive a food consumer selection identifying an end state of a food product, a package cooling sub-system comprising a chilled fluid bath, a gripper component configured to agitate a package containing the food product in the chilled fluid bath, and a controller configured to command the gripper to control the rate of heat transfer from the package to the chilled fluid bath based on receiving an input identifying an end state selection from the food consumer interface and based on receiving an input containing a value of the physical parameter of the food product from the gripper component.

Abstract: A turbine engine has: a compressor; a combustor; a turbine, a gas flowpath passing consecutively through the compressor, combustor, and turbine; and inlet member along the gas flowpath upstream of the compressor. The inlet member includes the unitarily-formed single piece combination of: a three dimensional (3D) lattice portion; and a nose cap body surrounding the lattice portion.

Abstract: An after-fan system for a gas turbine engine includes a variable pitch fan exit guide vane array. An after-fan turbine downstream of the variable pitch fan exit guide vane array and a control operable to vary a pitch of the variable fan exit guide vane array. A method of generating thrust for a gas turbine engine includes rotating a fan section with an array of fan blades; rotating an after-fan turbine downstream of the fan section; and varying a pitch of a variable fan exit guide vane array downstream of the fan section and upstream of the after-fan turbine.

Abstract: A laser hole drilling system includes a laser source that generates a laser beam along an optical axis; a spherical lens along the optical axis downstream of the laser source; and a control system in communication with the spherical lens and the laser source, the control system operable to locate the spherical lens with respect to the laser source to produce a light distribution in polar coordinates of a real portion of the Fourier Transform to generate an asymmetric teardrop shaped energy distribution at a focal plane.

Abstract: A laser hole drilling system. The system includes a laser source that generates a laser beam along an optical axis, a cylindrical lens along the optical axis downstream of the laser source, and a spherical lens downstream of the cylindrical lens, the spherical lens offset from the optical axis to provide an anamorphic optical train to generate an asymmetric teardrop shaped energy distribution at a focal plane.

Abstract: A thermal relief device (1) is described comprising a housing (2) having an inlet (3) and an outlet (4) connected by a relief channel (5). Such a thermal relief device should have a simple construction. To this end a microporous structure (10) is arranged between inlet (3) and outlet (4).

Abstract: A plasma generator includes a cylindrical containment housing, an anode in a confinement space within the containment housing and a cathode within the anode. The cylindrical containment housing includes an open end and a closed end. A base forms the closed end. That base includes a first gas inlet and a first gas outlet.

Abstract: The present invention provides improved methods for preparing polysaccharide protein conjugates utilizing surfactants to improve filterability of the conjugation reaction mixture. The polysaccharide-protein conjugates are useful for inclusion in vaccines.

Abstract: The present invention provides immunogenic compositions having one or more polysaccharide-protein conjugates in which polysaccharides obtained from bacterial capsules are conjugated to diphtheria toxin fragment B (DTFB) or a variant thereof. The immunogenic compositions may be multivalent pneumococcal polysaccharide conjugate compositions in which polysaccharides from one or more Streptococcus pneumoniae serotypes are conjugated to diphtheria toxin fragment B (DTFB) and, optionally, additional polysaccharides from one or more different Streptococcus pneumoniae serotypes are conjugated to one or more other carrier proteins.

Abstract: The present invention provides immunogenic compositions having one or more polysaccharide-protein conjugates in which one or more polysaccharides from Streptococcus pneumoniae bacterial capsules are conjugated to a carrier protein in an aprotic solvent such as dimethylsulfoxide (DMSO). The present invention also provides methods for providing an enhanced immune response to a pneumococcal polysaccharide protein conjugate vaccine comprising administering to a human subject an immunogenic composition comprising polysaccharide-protein conjugates prepared in DMSO conditions.