Abstract: The present invention relates to a user digital journey validation method comprising the steps of: connecting, by a fully distributed blockchain computer system, a trust network comprising: a plurality of user nodes; a plurality of trusted party nodes; a visitor node corresponding to a visitor to a digital service; and a plurality of links, the user nodes corresponding to users, the trusted party nodes corresponding to trusted parties, the visitor node being the most recent node in the trust network, the links being the connections between nodes; rating, by the trust network, the visitor node, the rating being a visitor trust score; recording, via a digital journey mapping system, a digital journey of the visitor; analysing, by the AI system, the digital journey of the visitor; detecting, by the AI system, bot-like behaviour associated with the visitor node or the user nodes; assigning, by the AI system, a warning flag to the visitor node or the user if the visitor node or user node has associated bot-like be

Abstract: A method of culturing non-transformed mammalian respiratory epithelial cells in vitro. The method includes the steps of harvesting the epithelial cells from the respiratory tract of a mammalian donor; suspending the epithelial cells in a fluid culture medium to form a cell suspension; introducing the cell suspension into a first reservoir in communication with an adherence substrate; introducing additional fluid culture medium into a second reservoir separated from the first reservoir by a fluid permeable baffle; incubating the cells under conditions suitable for promoting epithelial cell growth for a period of at least 72 hours; and changing the fluid culture medium within the second reservoir during the incubation period at intervals of approximately 24-72 hours without disturbing the epithelial cells within the first reservoir. Preferably, the epithelial cells are harvested from the nasal mucosa of a human donor using a minimally-invasive brushing technique.

Abstract: A method of culturing non-transformed mammalian respiratory epithelial cells in vitro. The method includes the steps of harvesting the epithelial cells from the respiratory tract of a mammalian donor; suspending the epithelial cells in a fluid culture medium to form a cell suspension; introducing the cell suspension into a first reservoir in communication with an adherence substrate; introducing additional fluid culture medium into a second reservoir separated from the first reservoir by a fluid permeable baffle; incubating the cells under conditions suitable for promoting epithelial cell growth for a period of at least 72 hours; and changing the fluid culture medium within the second reservoir during the incubation period at intervals of approximately 24-72 hours without disturbing the epithelial cells within the first reservoir. Preferably, the epithelial cells are harvested from the nasal mucosa of a human donor using a minimally-invasive brushing technique.