Patents by Inventor Michael Anthony Tangrea
Michael Anthony Tangrea has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200041489Abstract: Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.Type: ApplicationFiled: April 6, 2018Publication date: February 6, 2020Inventors: Michael R. EMMERT-BUCK, Michael Anthony TANGREA
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Patent number: 8597715Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: GrantFiled: April 2, 2010Date of Patent: December 3, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Patent number: 8460744Abstract: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.Type: GrantFiled: February 25, 2010Date of Patent: June 11, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Robert F. Bonner, Thomas J. Pohida, Michael R. Emmert-Buck, Michael Anthony Tangrea, Rodrigo F. Chuaqui
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Publication number: 20100216166Abstract: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.Type: ApplicationFiled: February 25, 2010Publication date: August 26, 2010Inventors: Robert F. Bonner, Thomas J. Pohida, Michael R. Emmert-Buck, Michael Anthony Tangrea, Rodrigo F. Chuaqui
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Publication number: 20100190177Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: ApplicationFiled: April 2, 2010Publication date: July 29, 2010Inventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Patent number: 7709047Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: GrantFiled: July 23, 2003Date of Patent: May 4, 2010Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Patent number: 7695752Abstract: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.Type: GrantFiled: August 12, 2005Date of Patent: April 13, 2010Assignee: The Government of the United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Robert F. Bonner, Thomas J. Pohida, Michael R. Emmert-Buck, Michael Anthony Tangrea, Rodrigo F. Chuaqui