Abstract: Disclosed herein are methods of generating a subpool of oligonucleotides from a larger pool of oligonucleotides. A subpool of oligonucleotides can contain a portion of a gene of interest, and can be generated on a subject by subject basis on demand. Also disclosed herein are methods of processing a subpool of oligonucleotides to generate probes for sequencing. Also disclosed herein are systems and methods for using subpools of oligonucleotides generated from a larger pool for high-throughput sequencing.

Abstract: A navigation system includes: a control unit configured to generate a feed hierarchy for prioritizing a trustworthiness of an interest feed based on a feed characteristic; determine a discrepancy by comparing the interest feed with the interest list, a feed record, or a combination thereof; determine a feed authorization based on the feed characteristic, the feed hierarchy, an entry category, or a combination thereof for manipulating the interest list; generate an update to the interest list based on the discrepancy, the feed hierarchy, and the feed authorization; and confirm the update to the interest list based on a navigation behavior at an interest location associated with the update as determined by a location unit coupled to the control unit.