Abstract: The present disclosure relates to health monitoring and maintenance of mobile platforms such as aircraft. In particular, onboard apparatus and methods and also ground-based apparatus and methods that cooperate in assisting with the maintenance of mobile platforms by facilitating diagnosis of events detected onboard mobile platforms while such mobile platforms are in operation (e.g., transit, flight) are disclosed. In various aspects, the present disclosure discloses apparatus and methods for handling and reporting the detection of events onboard mobile platforms, reporting predefined additional information associated with the event upon request from a ground facility, identifying one or more potential causes for the detected event and determining the occurrence probability for each potential cause identified.

Abstract: The present disclosure relates to health monitoring and maintenance of mobile platforms such as aircraft. In particular, onboard apparatus and methods and also ground-based apparatus and methods that cooperate in assisting with the maintenance of mobile platforms by facilitating diagnosis of events detected onboard mobile platforms while such mobile platforms are in operation (e.g., transit, flight) are disclosed. In various aspects, the present disclosure discloses apparatus and methods for handling and reporting the detection of events onboard mobile platforms, reporting predefined additional information associated with the event upon request from a ground facility, identifying one or more potential causes for the detected event and determining the occurrence probability for each potential cause identified.

Abstract: Disclosed is a method of screening a test substance for possession of binding activity for possession of binding activity for MSP42 or a fragment thereof, the method comprising the steps of: combining or contacting, in any order, (i) a molecule comprising MSP142 or a fragment thereof, (ii) the test substance, and (iii) a comparison substance known to have binding activity for MSP142 or a fragment thereof; and determining the presence and/or amount, if any, of comparison substance and/or test substance bound of the MSP142 or fragment thereof.

Abstract: Fluorogenic protease substrates are provided that comprise a peptide doubly labelled via thiol groups of the peptide with an alkyleneamidotetramethylrhodamine (alkyleneamido-TMR) group. Preferred substrates are doubly labelled with substantially pure 5-methyleneamido-TMR or 6-methyleneamido-TMR. Methods of preparing the substrates are provided, which comprise reacting the unlabelled peptide with haloalkylamido-TMR (preferably iodoacetamido-TMR). More generally, fluorogenic protease substrates are also provided which comprise a peptide doubly labelled with the same rhodamine derivative, where the two labels, and their linkages to the peptide, are substantially isomerically identical. Also provided are related methods for assaying protease activity in a sample, kits for use in such methods, and solid supports bearing the substrates of the invention.