Patents by Inventor Michael Borns

Michael Borns has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Transposase-random priming DNA sample preparation
    Patent number: 10240196
    Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: March 26, 2019
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Bahram Arezi, Michael Borns, Holly Hogrefe, Connie Hansen
  • Fast hybridization for next generation sequencing target enrichment
    Patent number: 10093962
    Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: October 9, 2018
    Assignee: Agilent Technologies, Inc.
    Inventor: Michael Borns
  • TRANSPOSASE-RANDOM PRIMING DNA SAMPLE PREPARATION
    Publication number: 20170342483
    Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
    Type: Application
    Filed: May 27, 2016
    Publication date: November 30, 2017
    Inventors: Bahram Arezi, Michael Borns, Holly Hogrefe, Connie Hansen
  • Fast hybridization for next generation sequencing target enrichment
    Patent number: 9587268
    Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
    Type: Grant
    Filed: January 29, 2014
    Date of Patent: March 7, 2017
    Assignee: Agilent Technologies Inc.
    Inventor: Michael Borns
  • FAST HYBRIDIZATION FOR NEXT GENERATION SEQUENCING TARGET ENRICHMENT
    Publication number: 20160017405
    Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
    Type: Application
    Filed: September 29, 2015
    Publication date: January 21, 2016
    Applicant: Agilent Technologies, Inc.
    Inventor: Michael Borns
  • High fidelity DNA polymerase compositions and uses thereof
    Patent number: 9181534
    Abstract: The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 5?-3? exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: November 10, 2015
    Assignee: Agilent Technologies Inc.
    Inventors: Holly Hogrefe, Michael Borns, Joseph A. Sorge
  • FAST HYBRIDIZATION FOR NEXT GENERATION SEQUENCING TARGET ENRICHMENT
    Publication number: 20150211047
    Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
    Type: Application
    Filed: January 29, 2014
    Publication date: July 30, 2015
    Applicant: Agilent Technologies, Inc.
    Inventor: Michael Borns
  • DNA binding protein-polymerase chimeras
    Patent number: 9040276
    Abstract: The invention relates to compositions and methods directed to chimeric DNA polymerases, which comprise a mutated DNA binding polypeptide domain and a mutated or wild-type DNA polymerase polypeptide domain.
    Type: Grant
    Filed: July 17, 2006
    Date of Patent: May 26, 2015
    Assignee: Agilent Technologies, Inc.
    Inventor: Michael Borns
  • DNA polymerase fusions and uses thereof
    Patent number: 8883454
    Abstract: The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.
    Type: Grant
    Filed: December 23, 2009
    Date of Patent: November 11, 2014
    Assignee: Agilent Technologies, Inc.
    Inventor: Michael Borns
  • Polymerase Enhancing Factor (PEF) Extracts PEF Protein Complexes Isolated PEF Proteins and Methods for Purifying and Identifying
    Publication number: 20120003713
    Abstract: The invention provides novel extracts, proteins, and complexes that improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. As an example, specifically described is nucleotide and amino acid sequence information for a Pyrococcus furiosus PEF (P45), which was used to produce a recombinant PEF.
    Type: Application
    Filed: March 18, 2011
    Publication date: January 5, 2012
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: Connie Hansen, Holly Hogrefe, Michael Borns, Michael Muhich
  • DNA polymerase blends and uses thereof
    Patent number: 7960157
    Abstract: The present invention discloses novel blends of chimeric and non-chimeric thermostable DNA polymerases for use in PCR, DNA sequencing and mutagenesis protocols. The invention allows for PCR reactions with shorter extension times that will facilitate PCR amplification of genomic DNA templates and improve the efficacy of long PCR.
    Type: Grant
    Filed: November 5, 2003
    Date of Patent: June 14, 2011
    Assignee: Agilent Technologies, Inc.
    Inventor: Michael Borns
  • Polymerase enhancing factor (PEF) extracts, PEF protein complexes, isolated PEF protein, and methods for purifying and identifying
    Patent number: 7943358
    Abstract: The invention provides novel extracts, proteins, and complexes that improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. As an example, specifically described is nucleotide and amino acid sequence information for a Pyrococcus furiousus PEF (P45), which was used to produce a recombinant PEF.
    Type: Grant
    Filed: December 4, 2006
    Date of Patent: May 17, 2011
    Assignee: Agilent Technologies, Inc.
    Inventors: Connie J. Hansen, Holly Hogrefe, Michael Borns, Michael Muhich
  • Reaction buffer composition for nucleic acid replication with packed DNA polymerases
    Patent number: 7939645
    Abstract: The invention relates to compositions, methods, and kits for nucleic acid replication, including polymerase chain reaction (PCR) and mutagenesis reactions. A buffer composition is provided which allows higher concentrations of DNA polymerase to be used, resulting in greater yield of amplified product and faster reaction kinetics.
    Type: Grant
    Filed: January 8, 2007
    Date of Patent: May 10, 2011
    Assignee: Agilent Technologies, Inc
    Inventor: Michael Borns
  • High fidelity DNA polymerase compositions and uses therefor
    Patent number: 7932070
    Abstract: The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3?-5? exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
    Type: Grant
    Filed: August 23, 2002
    Date of Patent: April 26, 2011
    Assignee: Agilent Technologies, Inc.
    Inventors: Holly Hogrefe, Michael Borns, Joseph A. Sorge
  • DNA polymerase fusions and uses thereof
    Publication number: 20100173365
    Abstract: The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.
    Type: Application
    Filed: December 23, 2009
    Publication date: July 8, 2010
    Inventor: Michael Borns
  • DNA polymerase fusions and uses thereof
    Patent number: 7704712
    Abstract: The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.
    Type: Grant
    Filed: March 19, 2004
    Date of Patent: April 27, 2010
    Assignee: Stratagene California
    Inventor: Michael Borns
  • DNA polymerase fusions and uses thereof
    Patent number: 7659100
    Abstract: The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.
    Type: Grant
    Filed: November 29, 2006
    Date of Patent: February 9, 2010
    Assignee: Stratagene California
    Inventor: Michael Borns
  • Zwitterionic detergents for the storage and use of DNA polymerases
    Publication number: 20080064071
    Abstract: The present invention provides methods, compositions, and kits for storing and enhancing the activity of thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic detergent or non-detergent surfactant. Compositions and kits for performing the process according to the invention are also provided.
    Type: Application
    Filed: July 25, 2007
    Publication date: March 13, 2008
    Inventors: Holly Hogrefe, Jeffrey Fox, Michael Borns, Jeffrey Braman
  • Reaction buffer composition for nucleic acid replication with packed DNA polymerases
    Publication number: 20080038782
    Abstract: The invention relates to compositions, methods, and kits for nucleic acid replication, including polymerase chain reaction (PCR) and mutagenesis reactions. A buffer composition is provided which allows higher concentrations of DNA polymerase to be used, resulting in greater yield of amplified product and faster reaction kinetics.
    Type: Application
    Filed: January 8, 2007
    Publication date: February 14, 2008
    Applicant: Stratagene California
    Inventor: Michael Borns
  • Transposase-random priming DNA sample preparation
    Patent number: RE49207
    Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
    Type: Grant
    Filed: November 8, 2019
    Date of Patent: September 13, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Bahram Arezi, Michael Borns, Holly Hogrefe, Connie Hansen