Abstract: The present teachings provide for methods of separating live and dead spermatozoa cells from non-human mammalian sex-selected semen. Sex-selected semen is first treated with proteinase K and TCEP, then applied to a density gradient media for separation. Separation is typically accomplished through centrifugation. Commercially available density gradient media include PERCOLL® and BOVIPURE®. Separation of live and dead cells in sex selected semen allows for precise quantification of sex skew using ddPCR™.

Abstract: An integrated system and method for preparing sperm cells to improve their survivability during cryopreservation are described herein. The system features a vessel, a controlled dispenser, and a dispense tube. The dispense tube has a first end fluidly connected to the dispenser and a second end disposed inside the vessel. The second end can be submerged in the sperm cell fluid. The controlled dispenser may be a syringe pump that includes a syringe for containing a cryoprotectant and a pushing mechanism for displacing the syringe. The syringe pump is configured to discharge the cryoprotectant through the dispense tube and into the vessel, thereby dispensing the cryoprotectant into the sperm cell fluid. Mixing of the fluids is achieved using a shaker table agitation.

Abstract: An integrated system and method for preparing sperm cells to improve their survivability during cryopreservation are described herein. The system features a vessel, a controlled dispenser, and a dispense tube. The dispense tube has a first end fluidly connected to the dispenser and a second end disposed inside the vessel. The second end can be submerged in the sperm cell fluid. The controlled dispenser may be a syringe pump that includes a syringe for containing a cryoprotectant and a pushing mechanism for displacing the syringe. The syringe pump is configured to discharge the cryoprotectant through the dispense tube and into the vessel, thereby dispensing the cryoprotectant into the sperm cell fluid. Mixing of the fluids is achieved using a shaker table agitation.

Abstract: An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Abstract: A securing device for securing a standstill of a vehicle, including a securing body having a bearing device for a movable bearing on the vehicle between a securing position (SP) and a release position (FP), and a counter-securing body having a fastening portion for a force-transmitting fastening to a wheel shaft of the vehicle. The counter-securing body includes at least one form-fitting portion for a form-fitting latching of a pawl portion of the securing body in its securing position (SP) and a release of the pawl portion of the securing body in its release position (FP).

Abstract: The present invention relates to an NADP(H) nanosensor comprising i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Abstract: A coryneform bacteria cell with an increased provision of Malonyl-CoA compared to its archetype, wherein the regulation and/or expression of one or more of genes fasB, gltA, accBC and accD1, and/or the functionality of the enzyme encoded by each gene is modified in a targeted manner. The cell may have one or more targeted modifications, including reduced or eliminated functionality of the fatty acid synthase FasB, mutation or partial or complete deletion of the fatty acid synthase encoding gene fasB, and/or reduced functionality of the promoter operatively linked to the citrate synthase gene gtIA, among other targeted modifications.

Abstract: A DNA sequence that includes at least 70% identity with respect to SEQ ID NO: 1 and further includes a triplet at position 1027-1029 that codes for alanine.

Abstract: A cable distribution plant is protected from noise where a modem housing encloses a switching power supply and digital electronics, the modem switching power supply for receiving AC mains power via an EMI filter and modem digital electronics for receiving a switching power supply output via an LC filter for filtering noise at the switching power supply frequency wherein multiple filters used with respective modems at subscriber sites protect the head-end from switching power supply harmonic noise otherwise aggregated by the nodes and passed to the head end.

Abstract: A securing device for securing a standstill of a vehicle, including a securing body having a bearing device for a movable bearing on the vehicle between a securing position (SP) and a release position (FP), and a counter-securing body having a fastening portion for a force-transmitting fastening to a wheel shaft of the vehicle. The counter-securing body includes at least one form-fitting portion for a form-fitting latching of a pawl portion of the securing body in its securing position (SP) and a release of the pawl portion of the securing body in its release position (FP).

Abstract: An integrated system and method for preparing sperm cells to improve their survivability during cryopreservation are described herein. The system features a vessel, a controlled dispenser, and a dispense tube. The dispense tube has a first end fluidly connected to the dispenser and a second end disposed inside the vessel. The second end can be submerged in the sperm cell fluid. The controlled dispenser may be a syringe pump that includes a syringe for containing a cryoprotectant and a pushing mechanism for displacing the syringe. The syringe pump is configured to discharge the cryoprotectant through the dispense tube and into the vessel, thereby dispensing the cryoprotectant into the sperm cell fluid. Mixing of the fluids is achieved using a shaker table agitation.

Abstract: A method of producing 5-ketofructose using an acetic acid bacterium which is genetically modified to express a fructose dehydrogenase as well as to microorganisms which can be used in said method.

Abstract: A cable distribution plant is protected from noise by a distributed cable modem filter.

Abstract: A DNA sequence that includes at least 70% identity with respect to SEQ ID NO: 1 and further includes a triplet at position 1027-1029 that codes for alanine.

Abstract: The present invention relates to an NADP(H) nanosensor comprising i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Abstract: A method for controlling a parking mode for a vehicle includes detecting an activation action of a user of the vehicle, determining a movement status of the vehicle, and activating a parking mode for the vehicle by closing a parking brake of a brake device of the vehicle and by engaging a gearshift lock of a transmission of the vehicle.

Abstract: A standstill securing concept for securing a standstill of a vehicle, having a transmission lock device for blocking a transmission movement of a drive transmission of the vehicle in a locking position and for enabling the transmission movement in an enablement position. The standstill securing concept further has a rotation-preventing securing device for blocking a wheel movement of one drive wheel of a drive axle of the vehicle in a park position and for enabling a wheel movement of the drive wheel in a drive position. An opposite drive wheel of the drive axle is configured without a device for rotation prevention.

Abstract: The present invention relates to an NADP(H) nanosensor comprising i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Abstract: A pneumatic cooling device including a housing having a first end and a second end. A handle extends from an outer surface of the housing. An inlet is positioned at substantially the first end of the housing and is configured to receive a supply of medical grade air into the housing. An outlet is positioned at substantially the second end of the housing and is configured to deliver the supply of medical grade air outside of the housing. A pathway extends through the housing between the inlet and the outlet and is configured to passively move the supply of medical grade air between the inlet and the outlet.

Abstract: A brake disk device for a vehicle having an annular brake disk with an encircling outer portion and an encircling inner portion, the inner diameter of which bounds a disk opening, and a brake pot with an annularly encircling edge portion, wherein the brake pot is fastened at the edge portion to the inner portion of the brake disk, wherein the edge portion is of multi-layered design at least in sections and/or is of thickened design in sections, wherein the walls of the edge portion are at least partially in contact, and an inner surface of the edge portion is designed as an additional brake surface.