Patents by Inventor Michael C. Holmes

Michael C. Holmes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods and compositions for treating hemophilia B
    Patent number: 9175280
    Abstract: Disclosed herein are methods and compositions for insertion of Factor IX (FIX) sequences into the genome of a cell for treating hemophilia B.
    Type: Grant
    Filed: October 12, 2011
    Date of Patent: November 3, 2015
    Assignees: Sangamo BioSciences, Inc., The Children's Hospital of Philadelphia
    Inventors: Philip D. Gregory, Katherine A. High, Michael C. Holmes, Hojun Li
  • Methods and compositions for alteration of a cystic fibrosis transmembrane conductance regulator (CFTR) gene
    Patent number: 9161995
    Abstract: Nucleases and methods of using these nucleases for alteration of a CFTR gene and generation of cells and animal models.
    Type: Grant
    Filed: July 25, 2012
    Date of Patent: October 20, 2015
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Dmitry M. Guschin, Michael C. Holmes, David Paschon, Phillip Tam
  • METHODS AND COMPOSITIONS FOR REGULATING HIV INFECTION
    Publication number: 20150267223
    Abstract: Methods and compositions for regulating HIV infection and/or replication in which an anti-HIV transgene is integrated into the genome of a cell using a nuclease.
    Type: Application
    Filed: May 28, 2015
    Publication date: September 24, 2015
    Inventors: Michael C. Holmes, Philip D. Gregory, Fyodor Urnov
  • METHODS AND COMPOSITIONS FOR MODIFICATION OF A HLA LOCUS
    Publication number: 20150252094
    Abstract: Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.
    Type: Application
    Filed: January 5, 2015
    Publication date: September 10, 2015
    Inventors: Trevor Collingwood, Laurence J.N. Cooper, Philip D. Gregory, Michael C. Holmes, Jeffrey C. Miller, Edward J. Rebar, Andreas Reik, Fyodor Urnov
  • Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
    Patent number: 9121072
    Abstract: Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications.
    Type: Grant
    Filed: September 15, 2011
    Date of Patent: September 1, 2015
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Michael C. Holmes, Yannick Doyon, Tianjian Li
  • METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION
    Publication number: 20150240263
    Abstract: Disclosed herein are methods and compositions for targeted, nuclease-mediated insertion of transgene sequences into the genome of a cell.
    Type: Application
    Filed: February 24, 2015
    Publication date: August 27, 2015
    Inventors: Michael C. Holmes, Thomas Wechsler
  • Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
    Patent number: 9115409
    Abstract: Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: August 25, 2015
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Yannick Doyon, Michael C. Holmes, Tianjian Li
  • LINEAR DONOR CONSTRUCTS FOR TARGETED INTEGRATION
    Publication number: 20150225727
    Abstract: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
    Type: Application
    Filed: April 29, 2015
    Publication date: August 13, 2015
    Inventors: Russell DeKelver, Philip D. Gregory, Michael C. Holmes, Fyodor Urnov
  • DELIVERY METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED GENOME ENGINEERING IN HEMATOPOIETIC STEM CELLS
    Publication number: 20150174169
    Abstract: The present disclosure is in the field of genome engineering, particularly targeted modification of the genome of a hematopoietic cell.
    Type: Application
    Filed: October 16, 2014
    Publication date: June 25, 2015
    Inventors: Pietro Genovese, Philip D. Gregory, Michael C. Holmes, Angelo Lombardo, Luigi Naldini
  • TARGETED DISRUPTION OF T CELL RECEPTOR GENES USING ENGINEERED ZINC FINGER PROTEIN NUCLEASES
    Publication number: 20150164954
    Abstract: Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
    Type: Application
    Filed: January 7, 2015
    Publication date: June 18, 2015
    Inventors: Maria Chiara Bonini, Pietro Genovese, Philip D. Gregory, Michael C. Holmes, Luigi Naldini, David Paschon, Elena Provasi, Lei Zhang
  • Linear donor constructs for targeted integration
    Patent number: 9045763
    Abstract: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for integration of an exogenous sequence into a specified region of interest in the genome of a cell.
    Type: Grant
    Filed: June 16, 2011
    Date of Patent: June 2, 2015
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Russell DeKelver, Philip D. Gregory, Michael C. Holmes, Fyodor Urnov
  • MODIFICATION OF CXCR4 USING ENGINEERED ZINC FINGER PROTEINS
    Publication number: 20150110757
    Abstract: Disclosed herein are methods and compositions for modulating activity of CXCR4 genes, for example using zinc finger transcription factors (ZF-TFs) or zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZF-TFs or ZFNs, vectors comprising polynucleotides encoding ZF-TFs or ZFNs and cells comprising polynucleotides encoding ZF-TFs or ZFNs and/or cells comprising ZF-TF or ZFNs are also provided.
    Type: Application
    Filed: September 24, 2014
    Publication date: April 23, 2015
    Inventors: Michael C. Holmes, Jeffrey C. Miller, Jianbin Wang
  • DELIVERY METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED GENOME ENGINEERING
    Publication number: 20150110762
    Abstract: The present disclosure is in the field of genome engineering, particularly targeted modification of the genome of a cell.
    Type: Application
    Filed: October 16, 2014
    Publication date: April 23, 2015
    Inventors: Michael C. Holmes, Jianbin Wang
  • Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
    Patent number: 8956828
    Abstract: Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
    Type: Grant
    Filed: November 10, 2010
    Date of Patent: February 17, 2015
    Assignees: Sangamo BioSciences, Inc., Ospedale San Raffaele S.R.L.
    Inventors: Maria Chiara Bonini, Pietro Genovese, Philip D. Gregory, Michael C. Holmes, Luigi Naldini, David Paschon, Elena Provasi, Lei Zhang
  • Methods and compositions for modification of a HLA locus
    Patent number: 8945868
    Abstract: Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: February 3, 2015
    Assignees: Sangamo BioSciences, Inc., Board of Regents, The University of Texas Systems
    Inventors: Trevor Collingwood, Laurence J. N. Cooper, Philip D. Gregory, Michael C. Holmes, Jeffrey C. Miller, Edward J. Rebar, Andreas Reik, Fyodor Urnov
  • Methods and compositions for targeted integration
    Patent number: 8936936
    Abstract: Disclosed herein are methods and compositions for targeted integration of one or more copies of a sequence of interest using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain and integrase defective lentiviral donor constructs.
    Type: Grant
    Filed: October 23, 2008
    Date of Patent: January 20, 2015
    Assignees: Sangamo BioSciences, Inc., Ospedale San Raffaele S.R.L.
    Inventors: Michael C. Holmes, Shuyuan Yao, Luigi Naldini, Angelo Leone Lombardo
  • METHODS AND COMPOSITIONS FOR TARGETED CLEAVAGE AND RECOMBINATION
    Publication number: 20140369980
    Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
    Type: Application
    Filed: July 31, 2014
    Publication date: December 18, 2014
    Inventors: Michael C. Holmes, Fyodor Urnov
  • Methods and compositions for modification of the locus
    Patent number: 8895264
    Abstract: Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: November 25, 2014
    Assignees: Sangamo BioSciences, Inc., The Regents of the University of California
    Inventors: Gregory J. Cost, Michael C. Holmes, Noriyuki Kasahara, Josee Laganiere, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Fyodor Urnov, Lei Zhang
  • DELIVERY METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED GENOME ENGINEERING
    Publication number: 20140335063
    Abstract: Disclosed herein are methods and compositions for delivery of engineered nucleases and donor molecules into the genome of a cell.
    Type: Application
    Filed: May 6, 2014
    Publication date: November 13, 2014
    Inventors: Paula M. Cannon, Colin Michael Exline, Michael C. Holmes
  • Modification of CXCR4 using engineered zinc finger proteins
    Patent number: 8871905
    Abstract: Disclosed herein are methods and compositions for modulating activity of CXCR4 genes, for example using zinc finger transcription factors (ZF-TFs) or zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZF-TFs or ZFNs, vectors comprising polynucleotides encoding ZF-TFs or ZFNs and cells comprising polynucleotides encoding ZF-TFs or ZFNs and/or cells comprising ZF-TF or ZFNs are also provided.
    Type: Grant
    Filed: March 18, 2010
    Date of Patent: October 28, 2014
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Michael C. Holmes, Jeffrey C. Miller, Jianbin Wang