Patents by Inventor Michael C. Pallas

Michael C. Pallas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10156509
    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
    Type: Grant
    Filed: June 13, 2012
    Date of Patent: December 18, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark Andersen, Michael C. Pallas, Haopeng Wang
  • Publication number: 20160208342
    Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample.
    Type: Application
    Filed: March 25, 2016
    Publication date: July 21, 2016
    Inventors: Gordon A. Janaway, Mark Andersen, Kornelija Zgonc, Michael C. Pallas, Marcin Sikora, Casey R. McFarland, Ferrier N. Le, Haopeng Wang, Jian Gong, Gothami Padmabandu
  • Patent number: 9273354
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: March 1, 2016
    Assignee: Illumina, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20150080247
    Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 19, 2015
    Inventors: Michael C. Pallas, James C. Nurse, Gary Lim, Theodore E. Straub, Eliodor Ghenciu, Evan W. Foster, Jorge Fonseca, Kevin Maher
  • Publication number: 20150072897
    Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/?15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 12, 2015
    Inventors: Michael C. Pallas, James C. Nurse, Gary Lim, Theodore E. Straub, Evan W. Foster
  • Publication number: 20150051118
    Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 19, 2015
    Inventors: Eliodor Ghenciu, Theodore E. Straub, Evan W. Foster, Michael C. Pallas
  • Publication number: 20140213464
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Application
    Filed: April 4, 2014
    Publication date: July 31, 2014
    Applicant: ILLUMINA, INC.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20140203169
    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
    Type: Application
    Filed: June 13, 2012
    Publication date: July 24, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark Andersen, Michael C. Pallas, Haopeng Wang
  • Patent number: 8728729
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: May 20, 2014
    Assignee: Illumina, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Patent number: 8361713
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: January 29, 2013
    Assignee: Illumina, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20090143244
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Application
    Filed: October 12, 2007
    Publication date: June 4, 2009
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20080285879
    Abstract: Methods, software, and apparatus for focusing an image in biological instrument are disclosed. Focusing elements are moved to various focus positions within a focus element travel range, and sample images are captured at the various focus positions. The sample images are resolved into subregions and an optimal focus position is determined based on the image intensity statistical dispersions within the identified subregions.
    Type: Application
    Filed: July 1, 2008
    Publication date: November 20, 2008
    Applicant: APPLIED BIOSYSTEMS INC.
    Inventors: Patrick D. Kinney, Howard G. King, Michael C. Pallas, Mark Naley
  • Patent number: 7394943
    Abstract: Methods, software, and apparatus for focusing an image in biological instrument are disclosed. Focusing elements are moved to various focus positions within a focus element travel range, and sample images are captured at the various focus positions. The sample images are resolved into subregions and an optimal focus position is determined based on the image intensity statistical dispersions within the identified subregions.
    Type: Grant
    Filed: June 30, 2004
    Date of Patent: July 1, 2008
    Assignee: Applera Corporation
    Inventors: Patrick D. Kinney, Howard G. King, Michael C. Pallas, Mark Naley
  • Patent number: 7282370
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: August 18, 2005
    Date of Patent: October 16, 2007
    Assignee: Solexa, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Patent number: 6969488
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: July 17, 2001
    Date of Patent: November 29, 2005
    Assignee: Solexa, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Sydney Brenner, Michael C. Pallas
  • Patent number: 6831994
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: July 17, 2001
    Date of Patent: December 14, 2004
    Assignee: Lynx Therapeutics, inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Patent number: 6806052
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: April 18, 2002
    Date of Patent: October 19, 2004
    Assignee: Lynx Therapeutics, Inc.
    Inventors: John Bridgham, Kevin P. Corcoran, George S. Golda, Michael C. Pallas, Sydney Brenner
  • Patent number: 6654505
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.
    Type: Grant
    Filed: July 17, 2001
    Date of Patent: November 25, 2003
    Assignee: Lynx Therapeutics, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20030077615
    Abstract: Abstract An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Application
    Filed: April 18, 2002
    Publication date: April 24, 2003
    Applicant: Lynx Therapeutics, Inc.
    Inventors: John Bridgham, Kevin P. Corcoran, George S. Golda, Michael C. Pallas, Sydney Brenner
  • Patent number: RE43097
    Abstract: The invention provides a method of nucleic acid sequence analysis based on the ligation of one or more sets of encoded adaptors to the terminus of a target polynucleotide. Encoded adaptors whose protruding strands form perfectly matched duplexes with the complementary protruding strands of the target polynucleotide are ligated, and the identity of the nucleotides in the protruding strands is determined by an oligonucleotide tag carried by the encoded adaptor. Such determination, or “decoding” is carried out by specifically hybridizing a labeled tag complement to its corresponding tag on the ligated adaptor.
    Type: Grant
    Filed: May 18, 2010
    Date of Patent: January 10, 2012
    Assignee: Illumina, Inc.
    Inventors: Glenn Albrecht, Sydney Brenner, Robert B. DuBridge, David H. Lloyd, Michael C. Pallas