Abstract: A disposable cassette for detecting nucleic acids or performing other assays. The cassette can be inserted into a base station during use. The cassette has numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. The vent pockets have protrusions to help prevent accidental resealing. The cassette also can have a gasket to ensure free air movement between open vent pockets. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with the vent pockets and the chambers.

Abstract: A method and device for isolating a migratory cell population from a mixed population of migratory cells and non-migratory cells is described herein. The method comprises placing the mixed population of migratory cells and non-migratory cells in contact with a device having one or more microchannels; and isolating the migratory cell population within the microchannels following an incubation period, wherein the one or more microchannels have an average size in a proliferation direction of the migratory cells that is less than a minimum proliferation space of the migratory cells along the proliferation direction.

Abstract: A genus of dihydropyridine chemical modulators of synaptojanin is disclosed. These modulators are selective inhibitors of synaptojanin 1 and may be used to treat cognitive impairment or traumatic brain injury, including promoting regeneration in cases of traumatic brain injury, or for treating neurodegenerative disorders.

Abstract: The present disclosure relates to compounds of Formula I: and pharmaceutically acceptable salts thereof, pharmaceutical compositions thereof, useful in the treatment of treating viral infections, for example, coronaviridae infections.

Abstract: The present disclosure provides to transparent and controllable human-AI interaction via chaining of machine-learned language models. In particular, although existing language models (e.g., so-called “large language models” (LLMs)) have demonstrated impressive potential on simple tasks, their breadth of scope, lack of transparency, and insufficient controllability can make them less effective when assisting humans on more complex tasks. In response, the present disclosure introduces the concept of chaining instantiations of machine-learned language models (e.g., LLMs) together, where the output of one instantiation becomes the input for the next, and so on, thus aggregating the gains per step.

Abstract: Provided herein are methods of treating a subject by administering a combination of an activatable cytokine construct (ACC) and a PD-1/PD-L1 pathway inhibitor in which the ACC includes: a first monomer construct including an optional first peptide mask (PM1), an optional third cleavable moiety (CM3), a first mature cytokine protein (CP1), a first cleavable moiety (CM1), and a first dimerization domain (DD1), wherein the CM1 is positioned between the CP1 and the DD1; and a second monomer construct including an optional second peptide mask (PM2), an optional forth cleavable moiety (CM4), a second mature cytokine protein (CP2), a second cleavable moiety (CM2), and a second dimerization domain (DD2), wherein the CM2 is positioned between the CP2 and the DD2; wherein the DD1 and the DD2 bind to each other thereby forming a dimer of the first monomer construct and the second monomer construct; and where the ACC is characterized by a reduction in at least one activity of the CP1 and/or CP2 as compared to a control l

Abstract: Electric submersible pumping systems are exposed to harsh chemicals in the downhole environment. Conductors used in the electric submersible pumping system must be shielded against these chemicals. To improve the resistance of conductors to hydrogen sulfide and other aggressive chemicals, an improved insulation system includes an interior layer applied to the conductor, a first outer layer, and an intermediate layer between the interior layer and the first outer layer. The intermediate layer can be a nano-composite layer that includes a mixture of nanoparticles in a polyether ether ketone (PEEK) polymer.

Abstract: A T-Cell receptor (TCR) specific embedding model uses a bidirectional long short-term memory (LSTM) to generate representations for TCR sequences and predict a “next token” in a TCR sequence. The embedding model can be trained in an unsupervised manner using a large collection of TCR sequences, and can be combined with downstream models to perform tasks, such as a TCR-epitope binding prediction model and a clustering algorithm. The embedding model demonstrates significant of prediction improvement when compared to existing models.

Abstract: A metals-based additive manufacturing machine and method are disclosed. The machine and method include a hybrid temperature monitoring system. The hybrid temperature monitoring system includes a Raman spectrometer, a single-element ultrasound transducer, and a phased-array ultrasound pair. The hybrid temperature monitoring system can generate a real-time three-dimensional temperature map of the melt pool and optionally a portion of the metal powder base and/or a formed portion of a desired artifact. The real-time three-dimensional temperature map can be used for optimizing the metals-based additive manufacturing process in real-time or during subsequent process runs.

Abstract: A metals-based additive manufacturing machine and method are disclosed. The machine and method include a hybrid temperature monitoring system. The hybrid temperature monitoring system includes a Raman spectrometer, a single-element ultrasound transducer, and a phased-array ultrasound pair. The hybrid temperature monitoring system can generate a real-time three-dimensional temperature map of the melt pool and optionally a portion of the metal powder base and/or a formed portion of a desired artifact. The real-time three-dimensional temperature map can be used for optimizing the metals-based additive manufacturing process in real-time or during subsequent process runs.

Abstract: A method of forming a layered material including arranging a 2DLM on a base material comprising one or more Moiré interferences, and adding material or removing material at a location of the one or more Moiré interferences.

Abstract: In one embodiment, a stirring system has a stirring assembly, a threaded leadscrew, and a multi-motion assembly adapted to travel along the leadscrew and accompanying parallel guide rods. The multi-motion assembly (i) has a timing pulley for powering the stirring assembly via a timing belt and (ii) converts the rotation of the leadscrew into either travel along the leadscrew in an engaged state or rotation of the timing pulley in a disengaged state. The multi-motion assembly includes (i) a screw-nut having an inside thread matching the leadscrew's thread and (ii) a clutch sub-assembly. The clutch sub-assembly (i) prevents the screw nut from rotating with the leadscrew in the engaged state, such that leadscrew rotation causes linear movement of the multi-motion assembly and (ii) allows the screw nut to rotate with the leadscrew in the disengaged state, such that leadscrew rotation causes corresponding rotation of the screw nut and timing pulley.

Abstract: A system, apparatus and method for identifying states or types of individual objects within a class of objects of interest are provided. A supervised algorithm is executed on a set of map data to separate a class of objects of interest from other objects. An unsupervised algorithm is executed to identify different types or states of individual objects within the class of objects of interest identified by the supervised algorithm. The results are then stored on a non-transitory storage medium.

Abstract: In one embodiment, a stirring system has a stirring assembly, a threaded leadscrew, and a multi-motion assembly adapted to travel along the leadscrew and accompanying parallel guide rods. The multi-motion assembly (i) has a timing pulley for powering the stifling assembly via a timing belt and (ii) converts the rotation of the leadscrew into either travel along the leadscrew in an engaged state or rotation of the timing pulley in a disengaged state. The multi-motion assembly includes (i) a screw-nut having an inside thread matching the leadscrew's thread and (ii) a clutch sub-assembly. The clutch sub-assembly (i) prevents the screw nut from rotating with the leadscrew in the engaged state, such that leadscrew rotation causes linear movement of the multi-motion assembly and (ii) allows the screw nut to rotate with the leadscrew in the disengaged state, such that leadscrew rotation causes corresponding rotation of the screw nut and timing pulley.

Abstract: In one embodiment, a washing system including a nozzle assembly having a nozzle and having formed therein a rinse passageway and a waste passageway. Each passageway has first and second ends. The first end of the waste passageway is adapted to be coupled to a waste pump adapted to remove waste fluid via a waste aperture defining the second end of the waste passageway. The first end of the rinse passageway is adapted to be coupled to a rinse pump adapted to provide rinse fluid via a rinse aperture defining the second end of the rinse passageway. The nozzle is coupled to the rinse aperture and is adapted to rotate and direct flow of the rinse fluid outwardly while rotating.

Abstract: In one embodiment, a washing system including a nozzle assembly having a nozzle and having formed therein a rinse passageway and a waste passageway. Each passageway has first and second ends. The first end of the waste passageway is adapted to be coupled to a waste pump adapted to remove waste fluid via a waste aperture defining the second end of the waste passageway. The first end of the rinse passageway is adapted to be coupled to a rinse pump adapted to provide rinse fluid via a rinse aperture defining the second end of the rinse passageway. The nozzle is coupled to the rinse aperture and is adapted to rotate and direct flow of the rinse fluid outwardly while rotating.

Abstract: In one embodiment, a washing system including a nozzle assembly having a nozzle and having formed therein a rinse passageway and a waste passageway. Each passageway has first and second ends. The first end of the waste passageway is adapted to be coupled to a waste pump adapted to remove waste fluid via a waste aperture defining the second end of the waste passageway. The first end of the rinse passageway is adapted to be coupled to a rinse pump adapted to provide rinse fluid via a rinse aperture defining the second end of the rinse passageway. The nozzle is coupled to the rinse aperture and is adapted to rotate and direct flow of the rinse fluid outwardly while rotating.

Abstract: In one embodiment, a washing system including a nozzle assembly having a nozzle and having formed therein a rinse passageway and a waste passageway. Each passageway has first and second ends. The first end of the waste passageway is adapted to be coupled to a waste pump adapted to remove waste fluid via a waste aperture defining the second end of the waste passageway. The first end of the rinse passageway is adapted to be coupled to a rinse pump adapted to provide rinse fluid via a rinse aperture defining the second end of the rinse passageway. The nozzle is coupled to the rinse aperture and is adapted to rotate and direct flow of the rinse fluid outwardly while rotating.

Abstract: Improved sampler apparatus and methods for dissolution testing include a controller that determines whether several different dissolution testing methods can be performed concurrently by the sampler and controls the sampler in response to the determination; fluid delivery apparatus adapted to pierce a septum and to deliver fluid to or from a collection receptacle through a first aperture, and to permit air flow to or from the collection receptacle through a second aperture; a syringe pump including a distributor having apertures through which fluid flows into and out of the pump chamber, the piston of the syringe pump being moveable adjacent the apertures; and/or a leak sensor responsive to the presence of spilled fluids.

Abstract: Improved sampler apparatus and methods for dissolution testing include a controller that determines whether several different dissolution testing methods can be performed concurrently by the sampler and controls the sampler in response to the determination; fluid delivery apparatus adapted to pierce a septum and to deliver fluid to or from a collection receptacle through a first aperture, and to permit air flow to or from the collection receptacle through a second aperture; a syringe pump including a distributor having apertures through which fluid flows into and out of the pump chamber, the piston of the syringe pump being moveable adjacent the apertures; and/or a leak sensor responsive to the presence of spilled fluids.