Patents by Inventor Michael Christopher Montalto
Michael Christopher Montalto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9518982Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.Type: GrantFiled: November 3, 2009Date of Patent: December 13, 2016Assignee: GENERAL ELECTRIC COMPANYInventors: Anup Sood, Michael Christopher Montalto, Michael Gerdes, Maximilian Lewis Seel, Robert John Filkins
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Patent number: 8822147Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.Type: GrantFiled: April 27, 2012Date of Patent: September 2, 2014Assignee: General Electric CompanyInventors: Anup Sood, Michael Christopher Montalto, Michael Gerdes, Maximilian Lewis Seel, Robert John Filkins
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Publication number: 20140045251Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.Type: ApplicationFiled: October 23, 2013Publication date: February 13, 2014Applicant: General Electric CompanyInventors: Anup Sood, Michael Christopher Montalto, Michael John Gerdes, Maximilian Lewis Seel, Robert John Filkins
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Publication number: 20120231967Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.Type: ApplicationFiled: April 27, 2012Publication date: September 13, 2012Applicant: GENERAL ELECTRIC COMPANYInventors: Anup Sood, Michael Christopher Montalto, Michael Gerdes, Maximilian Lewis Seel, Robert John Filkins
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Publication number: 20120135449Abstract: Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent, and releasing the sample from the flow cell wherein the integrity of the sample is intact. The process of staining, combining and flowing may be iteratively repeated.Type: ApplicationFiled: February 3, 2012Publication date: May 31, 2012Applicant: GENERAL ELECTRIC COMPANYInventors: Jun Xie, Robert John Filkins, Fiona Mary Ginty, Michael Christopher Montalto, Anup Sood, Jeffrey Bernard Fortin, Wei-Cheng Tian, Michael John Gerdes
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Patent number: 8114382Abstract: The present invention relates to methods for the detection of myelin and a quantitative measurement of its local concentration in a sample using a benzofuran compound or its radioisotope derivatives. In one embodiment a method of myelin detection comprises identifying a subject at risk of or diagnosed with a myelin-associated neuropathy, parenterally administering to the subject the benzofuran compound, or a derivative of the compound, and determining myelination in the subject by detecting binding in the sample. In one embodiment, the invention provides a method of imaging myelinated tissue in a surgical field of mammalian tissue comprising the steps of contacting the surgical site with the benzofuran compound or a radioisotope derivative of the compound, and detecting binding in the surgical site.Type: GrantFiled: September 17, 2008Date of Patent: February 14, 2012Assignee: General Electric CompanyInventors: Cristina Abucay Tan Hehir, Stephen Johnson Lomnes, Kenneth Michael Fish, Tiberiu Mircea Siclovan, Michael Christopher Montalto, Tunchiao Hubert Lam
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Publication number: 20110305635Abstract: The present invention relates to methods for the detection of myelin and a quantitative measurement of its local concentration in a sample using a benzofuran compound or its radioisotope derivatives. In one embodiment a method of myelin detection comprises identifying a subject at risk of or diagnosed with a myelin-associated neuropathy, parenterally administering to the subject the benzofuran compound, or a derivative of the compound, and determining myelination in the subject by detecting binding in the sample. In one embodiment, the invention provides a method of imaging myelinated tissue in a surgical field of mammalian tissue comprising the steps of contacting the surgical site with the benzofuran compound or a radioisotope derivative of the compound, and detecting binding in the surgical site.Type: ApplicationFiled: September 17, 2008Publication date: December 15, 2011Applicant: GENERAL ELECTRIC COMPANYInventors: Christina Abucay Tan Hehir, Stephen Johnson Lomnes, Kenneth Michael Fish, Tiberiu Mircea Siclovan, Michael Christopher Montalto, Tunchiao Hubert Lam
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Patent number: 8060348Abstract: A system for analyzing tissue samples, that generally comprises, a storage device for at least temporarily storing one or more images of one or more cells, wherein the images comprise a plurality of channels; and a processor that is adapted to determine the extent to which a biomarker may have translocated from at least one subcellular region to another subcellular region; and then to generate a score corresponding to the extent of translocation.Type: GrantFiled: November 30, 2006Date of Patent: November 15, 2011Assignee: General Electric CompanyInventors: Harvey Ellis Cline, Ali Can, Xiaodong Tao, Robert John Filkins, Maximilian Lewis Seel, Michael Christopher Montalto
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Patent number: 7837981Abstract: Provided herein are agents that bind to soluble beta-amyloid. Also provided are in vivo and in vitro methods for detecting soluble beta-amyloid in a sample that may include brain tissue.Type: GrantFiled: December 11, 2006Date of Patent: November 23, 2010Assignee: General Electric CompanyInventors: Tiberiu Mircea Siclovan, Michael Christopher Montalto, Kenneth Michael Fish, Eric Dustin Agdeppa, Cristina Tan Hehir
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Patent number: 7745222Abstract: Provided herein are amyloid-binding assays that simulate in situ brain conditions by combining binders with various A-beta species on mammalian brain tissue. In general the amyloid-binding assays comprise the steps of (a) contacting a mammalian brain tissue sample with at least one A-beta species; (b) applying a putative binder to the brain tissue sample; and (c) determining whether the putative binder binds to the A-beta species.Type: GrantFiled: October 13, 2006Date of Patent: June 29, 2010Assignee: General Electric CompanyInventors: Kenneth Michael Fish, Cristina Tan Hehir, Michael Christopher Montalto, Eric Dustin Agdeppa
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Patent number: 7741045Abstract: Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.Type: GrantFiled: September 28, 2007Date of Patent: June 22, 2010Assignee: General Electric CompanyInventors: Michael J. Gerdes, Anup Sood, Michael Christopher Montalto, Ali Can, Fiona Ginty, Maureen Ann Bresnahan, Robert John Filkins, Zhengyu Pang
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Patent number: 7741046Abstract: Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets adhered to a solid support, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The method further includes the steps of oxidizing the bound fluorescent probe with a solution containing an oxidizing agent that substantially inactivates the fluorescent probe, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.Type: GrantFiled: September 28, 2007Date of Patent: June 22, 2010Assignee: General Electric CompanyInventors: Melinda Larsen, Anup Sood, Michael J. Gerdes, Michael Christopher Montalto, Zhengyu Pang, Fiona Ginty
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Patent number: 7727511Abstract: Provided herein are agents that bind to soluble beta-amyloid. Also provided are in vivo and in vitro methods for detecting soluble beta-amyloid in a sample that may include brain tissue.Type: GrantFiled: December 11, 2006Date of Patent: June 1, 2010Assignee: General Electric CompanyInventors: Tiberiu Mircea Siclovan, Michael Christopher Montalto, Tun Chiao Hubert Lam, Eric Dustin Agdeppa, Amy Casey Williams, Cristina Tan Hehir
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Publication number: 20100068140Abstract: The present invention relates to methods for the detection of myelin and a quantitative measurement of its local concentration in a sample using a benzofuran compound or its radioisotope derivatives. In one embodiment a method of myelin detection comprises identifying a subject at risk of or diagnosed with a myelin-associated neuropathy, parenterally administering to the subject the benzofuran compound, or a derivative of the compound, and determining myelination in the subject by detecting binding in the sample. In one embodiment, the invention provides a method of imaging myelinated tissue in a surgical field of mammalian tissue comprising the steps of contacting the surgical site with the benzofuran compound or a radioisotope derivative of the compound, and detecting binding in the surgical site.Type: ApplicationFiled: September 17, 2008Publication date: March 18, 2010Applicant: GENERAL ELECTRIC COMPANYInventors: Christina Abucay Tan Hehir, Stephen Johnson Lomnes, Kenneth Michael Fish, Tiberiu Mircea Siclovan, Michael Christopher Montalto, Tunchiao Hubert Lam
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Publication number: 20100047925Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.Type: ApplicationFiled: November 3, 2009Publication date: February 25, 2010Applicant: GENERAL ELECTRIC COMPANYInventors: Anup Sood, Michael Christopher Montalto, Michael Gerdes, Maximilian Lewis Seel, Robert John Filkins
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Patent number: 7629125Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.Type: GrantFiled: November 16, 2006Date of Patent: December 8, 2009Assignee: General Electric CompanyInventors: Anup Sood, Michael Christopher Montalto, Michael Gerdes, Maximilian Lewis Seel, Robert John Filkins
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Publication number: 20090253163Abstract: Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, and flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.Type: ApplicationFiled: April 2, 2008Publication date: October 8, 2009Applicant: GENERAL ELECTRIC COMPANYInventors: Jun Xie, Fiona Ginty, Robert John Filkins, Michael Christopher Montalto, Anup Sood, Jeffrey Bernard Fortin, Wei-Cheng Tian, Michael John Gerdes
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Publication number: 20090162280Abstract: Agents, methods, and kits for qualitatively or quantitatively determining the presence of soluble A-beta in source of A-beta are provided, wherein the agents comprise the compound of formula III or the compound of formula III labeled with a signal generator.Type: ApplicationFiled: December 20, 2007Publication date: June 25, 2009Applicant: GENERAL ELECTRIC COMPANYInventors: Cristina Abucay Tan Hehir, Tiberiu Mircea Siclovan, Tunchiao Hubert Lam, Michael Christopher Montalto, Richard Waltz Harper, Warren Jaye Porter
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Publication number: 20080274473Abstract: Provided herein are amyloid-binding assays that simulate in situ brain conditions by combining binders with various A-beta species on mammalian brain tissue. In general the amyloid-binding assays comprise the steps of (a) contacting a mammalian brain tissue sample with at least one A-beta species; (b) applying a putative binder to the brain tissue sample; and (c) determining whether the putative binder binds to the A-beta species.Type: ApplicationFiled: October 13, 2006Publication date: November 6, 2008Inventors: Kenneth Michael Fish, Cristina Tan Hehir, Michael Christopher Montalto, Eric Dustin Agdeppa
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Publication number: 20080118916Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.Type: ApplicationFiled: November 16, 2006Publication date: May 22, 2008Applicant: GENERAL ELECTRIC COMPANYInventors: ANUP SOOD, MICHAEL CHRISTOPHER MONTALTO, MICHAEL GERDES, MAXIMILIAN LEWIS SEEL, ROBERT JOHN FILKINS