Abstract: Disclosed herein are compositions and methods related to isolated cells (e.g., isolated stem cells) comprising a disruption in the 3?-UTR of an immunosuppressor, cells differentiated from such stem cells (e.g., pancreatic islet cells or immune cells) and methods of using the cells to treat diseases (e.g., diabetes or cancer). Methods of producing (i.e., genetically modifying) the isolated cells (e.g., isolated stem cells) are also provided.

Abstract: A slot machine, gaming method, and program product are provided to dynamically display visual and audio content to be played in synchronization with a music track. A digital signal processor performs frequency analysis of background music as it is played, and identifies musical beats to create beat events. The beat event is used to trigger additional presentation elements such as visual effects, sounds, and lighting on the beat of the background music. Spectral content information from the frequency analysis is used to create dynamic background graphics.

Abstract: A slot machine, gaming method, and program product are provided to dynamically display visual and audio content to be played in synchronization with a music track. A digital signal processor performs frequency analysis of background music as it is played, and identifies musical beats to create beat events. The beat event is used to trigger additional presentation elements such as visual effects, sounds, and lighting on the beat of the background music. Spectral content information from the frequency analysis is used to create dynamic background graphics.

Abstract: The present disclosure relates to cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.

Abstract: Cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator, are provided. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.

Abstract: A slot machine, gaming method, and program product are provided to dynamically display visual and audio content to be played in synchronization with a music track. A digital signal processor performs frequency analysis of background music as it is played, and identifies musical beats to create beat events. The beat event is used to trigger additional presentation elements such as visual effects, sounds, and lighting on the beat of the background music. Spectral content information from the frequency analysis is used to create dynamic background graphics.

Abstract: Cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator, are provided. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.

Abstract: Methods and apparatus are described for implementing service discovery protocols on subnetted zero configuration networks. A process for managing service advertisement across a plurality of subnets may comprise: collecting service advertisements on a local network level by designated network devices; sending listings of services from each of the designated devices to a master network device; sending a table of services for the plurality of subnets from the master device to all of the designated devices on the plurality of subnets; creating by each of the designated network devices for the corresponding subnet a service discovery proxy table listing the service advertisements on the subnets of the plurality of subnets beyond the subnet corresponding to the designated device; and periodically transmitting by each of the designated devices on the corresponding subnets service advertisements for the services of the corresponding service discovery proxy table.

Abstract: An automated method for culturing stem cells using a robotic liquid handling system including a translatable bed and a movable multi-channel pipette. The method includes the steps of: locating a first multi-well cell culture plate and a multi-trough plate on the bed; placing a suspension of stem cells in at least one trough of the multi-trough plate; using the multi-channel pipette, transferring a portion of the suspension of stem cells to each well of the first multi-well cell culture plate such that at least two of the wells of the first multi-well cell culture plate have different densities of stem cells; selecting a well of the first multi-well cell culture plate having a desired density of stem cells; locating a second multi-well cell culture plate on the bed; and using the multi-channel pipette, transferring the cells of the selected well to a plurality of wells of the second multi-well cell culture plate.

Abstract: An automated method for culturing stem cells using a robotic liquid handling system including a translatable bed and a movable multi-channel pipette. The method includes the steps of: locating a first multi-well cell culture plate and a multi-trough plate on the bed; placing a suspension of stem cells in at least one trough of the multi-trough plate; using the multi-channel pipette, transferring a portion of the suspension of stem cells to each well of the first multi-well cell culture plate such that at least two of the wells of the first multi-well cell culture plate have different densities of stem cells; selecting a well of the first multi-well cell culture plate having a desired density of stem cells; locating a second multi-well cell culture plate on the bed; and using the multi-channel pipette, transferring the cells of the selected well to a plurality of wells of the second multi-well cell culture plate.

Abstract: Safety apparatuses may include an apparatus housing configured for deployment in the at least one protected area. A CPU may be provided in the apparatus housing. At least one power supply may interface with the CPU. At least one caution light may interface with the CPU. The at least one caution light may be provided on an exterior of the apparatus housing, whereby the CPU illuminates the at least one caution light and the at least one caution light notifies or alerts the drivers to the persons in the at least one protected area. Safety methods are also disclosed.

Abstract: An automated method for culturing stem cells using a robotic liquid handling system including a translatable bed and a movable multichannel pipette. The method includes the steps of: locating a first multi-well cell culture plate and a multi-trough plate on the bed; placing a suspension of stem cells in at least one trough of the multi-trough plate; using the multi-channel pipette, transferring a portion of the suspension of stem cells to each well of the first multi-well cell culture plate such that at least two of the wells of the first multi-well cell culture plate have different densities of stem cells; selecting a well of the first multi-well cell culture plate having a desired stem cell density; locating a second multi-well cell culture plate; and using the multi-channel pipette, transferring the cells of the selected well to a plurality of wells of the second multi-well cell culture plate.

Abstract: Methods and apparatus are described for implementing service discovery protocols on subnetted zero configuration networks. A process for managing service advertisement across a plurality of subnets may comprise: collecting service advertisements on a local network level by designated network devices; sending listings of services from each of the designated devices to a master network device; sending a table of services for the plurality of subnets from the master device to all of the designated devices on the plurality of subnets; creating by each of the designated network devices for the corresponding subnet a service discovery proxy table listing the service advertisements on the subnets of the plurality of subnets beyond the subnet corresponding to the designated device; and periodically transmitting by each of the designated devices on the corresponding subnets service advertisements for the services of the corresponding service discovery proxy table.

Abstract: Methods and apparatus are described for implementing service discovery protocols on subnetted zero configuration networks. A process for managing service advertisement across a plurality of subnets may comprise: collecting service advertisements on a local network level by designated network devices; sending listings of services from each of the designated devices to a master network device; sending a table of services for the plurality of subnets from the master device to all of the designated devices on the plurality of subnets; creating by each of the designated network devices for the corresponding subnet a service discovery proxy table listing the service advertisements on the subnets of the plurality of subnets beyond the subnet corresponding to the designated device; and periodically transmitting by each of the designated devices on the corresponding subnets service advertisements for the services of the corresponding service discovery proxy table.