Patents by Inventor Michael D. Topal

Michael D. Topal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of cleaving DNA
    Patent number: 5418150
    Abstract: A method of cleaving substrate DNA with a restriction enzyme, wherein the substrate DNA is resistant to cleavage by the restriction enzyme, is disclosed. The method comprises co-incubating the substrate DNA and the restriction enzyme with an activating DNA sequence. The activating sequence comprises an oligonucleotide comprised of the restriction enzyme recognition site and cleavage permissible flanking sequences joined directly to both the 5' and 3' ends of the recognition site. Exemplary restriction enzymes which may be used in practicing the present invention include Nae I, BspM I, Hpa II, Nar I, and Sac II.
    Type: Grant
    Filed: September 21, 1993
    Date of Patent: May 23, 1995
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Michael D. Topal, Michael J. Conrad
  • Method of cleaving DNA
    Patent number: 5248600
    Abstract: A method of cleaving substrate DNA with a restriction enzyme, wherein the substrate DNA is resistant to cleavage by the restriction enzyme, is disclosed. The method comprises co-incubating the substrate DNA and the restriction enzyme with an activating DNA sequence. The activating sequence comprises an oligonucleotide comprised of the restriction enzyme recognition site and cleavage permissible flanking sequences joined directly to both the 5' and 3' ends of the recognition site. Exemplary restriction enzymes which may be used in practicing the present invention include Nae I, BspM I, Hpa II, Nar I, and Sac II.
    Type: Grant
    Filed: December 14, 1990
    Date of Patent: September 28, 1993
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Michael D. Topal, Michael J. Conrad