Patents by Inventor Michael Dauner
Michael Dauner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220204966Abstract: Evolved strains of Bifidobacterium and Lactobacillus are provided. The evolved strains have probiotic properties and have enhanced storage stability in both wet and dry conditions. The strains of the invention contain key genetic mutations and phenotypic alterations as compared with wild-type.Type: ApplicationFiled: April 23, 2020Publication date: June 30, 2022Inventors: Michael DAUNER, Gerda SAXER QUANCE, Steven Keith WILLIAMS
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Patent number: 10006058Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: GrantFiled: December 12, 2016Date of Patent: June 26, 2018Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Patent number: 9944954Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.Type: GrantFiled: August 4, 2016Date of Patent: April 17, 2018Assignee: Butamax Advanced Biofuels LLCInventors: Ritu Bhalla, Gopal K. Chotani, Michael Dauner, Mark J. Nelson, Daniel P. O'Keefe, Caroline M. Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb
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Patent number: 9771602Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products. Host cells provided herein comprise a pyruvate-utilizing pathway and a competitive growth advantage over other microorganisms in solution.Type: GrantFiled: March 14, 2014Date of Patent: September 26, 2017Assignee: Butamax Advanced Biofuels LLCInventors: Larry Cameron Anthony, Michael Dauner
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Patent number: 9701989Abstract: Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: GrantFiled: December 18, 2013Date of Patent: July 11, 2017Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Quinn Qun Zhu, Seung-Pyo Hong, Pamela L Sharpe, Narendra S Yadav, Xiaochun Fan, Michael Dauner
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Publication number: 20170088863Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: ApplicationFiled: December 12, 2016Publication date: March 30, 2017Inventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Patent number: 9550999Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: GrantFiled: July 18, 2014Date of Patent: January 24, 2017Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Publication number: 20160376613Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.Type: ApplicationFiled: August 4, 2016Publication date: December 29, 2016Inventors: RITU BHALLA, GOPAL K. CHOTANI, MICHAEL DAUNER, MARK J. NELSON, DANIEL P. O'KEEFE, CAROLINE M. PERES, JAHNAVI CHANDRA PRASAD, JEAN-FRANCOIS TOMB
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Publication number: 20160326552Abstract: The invention relates, for example, to recombinant yeast cells for differential gene expression during the propagation and production phases of a fermentation-based production process, as well as methods for using the same.Type: ApplicationFiled: December 22, 2014Publication date: November 10, 2016Inventors: Michael DAUNER, Brian James PAUL
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Patent number: 9441209Abstract: Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: GrantFiled: March 17, 2015Date of Patent: September 13, 2016Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Quinn Qun Zhu, Seung-Pyo Hong, Dongming Xie, Zhixiong Xue, Hyeryoung Yoon, Michael Dauner
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Patent number: 9441250Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.Type: GrantFiled: March 13, 2014Date of Patent: September 13, 2016Assignee: Butamax Advanced Biofuels LLCInventors: Ritu Bhalla, Gopal K Chotani, Michael Dauner, Mark J Nelson, Daniel P O'Keefe, Caroline M Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb
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Patent number: 9181566Abstract: The invention relates to suitable screening strategies for evaluating various candidate promoters for differential gene expression during the propagation and production phases of a fermentation process. The invention also relates to recombinant host cells that comprise identified promoter nucleic acid sequences and methods for producing fermentation products employing the same.Type: GrantFiled: December 28, 2012Date of Patent: November 10, 2015Assignee: BUTAMAX ADVANCED BIOFUELS LLCInventors: Michael Dauner, Arthur Leo Kruckeberg, Robert A Larossa, Brian James Paul, Wonchul Suh, Joseph Frederich Tuminello
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Publication number: 20150218526Abstract: Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: ApplicationFiled: March 17, 2015Publication date: August 6, 2015Inventors: Quinn Qun Zhu, Seung-Pyo Hong, Dongming Xie, Zhixiong Xue, Hyeryoung Yoon, Michael Dauner
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Publication number: 20150218597Abstract: The invention relates generally to the field of industrial microbiology and alcohol production. More specifically, the invention relates to the use of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) to improve butanol production. Butanol production can be improved by providing sufficient amounts of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) in the production media.Type: ApplicationFiled: March 16, 2015Publication date: August 6, 2015Inventors: MICHAEL DAUNER, MEHMEDALIJA JAHIC
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Patent number: 9012190Abstract: The invention relates generally to the field of industrial microbiology and alcohol production. More specifically, the invention relates to the use of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) to improve butanol production. Butanol production can be improved by providing sufficient amounts of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) in the production media.Type: GrantFiled: June 15, 2011Date of Patent: April 21, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Mehmedalija Jahic
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Patent number: 9005940Abstract: Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: GrantFiled: December 18, 2013Date of Patent: April 14, 2015Assignee: E I du Pont de Nemours and CompanyInventors: Quinn Qun Zhu, Seung-Pyo Hong, Dongming Xie, Zhixiong Xue, Hyeryoung Yoon, Michael Dauner
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Publication number: 20140349349Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: ApplicationFiled: July 18, 2014Publication date: November 27, 2014Inventors: MICHAEL DAUNER, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Patent number: 8871488Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: GrantFiled: June 15, 2011Date of Patent: October 28, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Publication number: 20140273130Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products. Host cells provided herein comprise a pyruvate-utilizing pathway and a competitive growth advantage over other microorganisms in solution.Type: ApplicationFiled: March 14, 2014Publication date: September 18, 2014Applicant: Butamax Advanced Biofuels LLCInventors: Larry Cameron ANTHONY, Michael Dauner
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Publication number: 20140273129Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Applicant: BUTAMAX ADVANCED BIOFUELS LLCInventors: Ritu Bhalla, Gopal K. Chotani, Michael Dauner, Mark J. Nelson, Daniel P. O'keefe, Caroline M. Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb