Abstract: Aspects relate to systems and methods for high-contrast cleavage detection. An exemplary method includes a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent, a waveguide configured to propagate an electromagnetic (EM) wave, wherein the waveguide includes a surface upon which the plurality of probes are immobilized, wherein the surface is configured to provide communication between the EM wave and the high-contrast agent, a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent and the plurality of probes are configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte, and a sensor in communication with the waveguide and configured to detect cleaving of the at least a probe by way of the EM wave.

Abstract: An apparatus for combined digital and optical processing of a cryptocurrency data block includes a digital processor that computes a hash vector from the cryptocurrency data block; a laser and splitter that produces optical input signals; optical modulators that binary phase-shift key modulate the optical input signals based on the hash vector; a photonic matrix multiplier circuit that performs an optically perform a discrete matrix-vector product operation on the modulated optical input signals to produce optical output signals, where the discrete matrix-vector product operation is defined by matrix elements limited to K discrete values, where 2?K?17; and photodetectors and comparators that perform optoelectronic conversions of the optical output signals to produce corresponding digital electronic output signals. The digital processor performs a second hash computation on an XOR result between the digital electronic output signals and the hash vector to produce a proof of work result.

Abstract: Disclosed herein are methods of allergen testing and IgG depletion. In some embodiments, testing for IgE antibodies specific for an allergen may be improved by first depleting IgG in a sample. In some embodiments, IgG may be depleted using Biotinylated Protein G (bPG) and streptavidin (SA).

Abstract: An apparatus for detecting an analyte, the apparatus includes a biosensor having at least a sensor surface functionalized with a binding ligand, wherein the at least a sensor surface is configured to selectively bind to an analyte, a microfluidic device configured to receive a sample fluid containing the analyte, incubate the biosensor with the sample fluid, and conjugate an anti-analyte molecule with a nanoparticle, wherein the nanoparticle is configured to provide a binding signal to the biosensor when the nanoparticle binds to the analyte bound to the binding ligand on the at least a sensor surface, and incubate the biosensor with the analyte bound to the binding ligand on the at least a sensor surface with the anti-analyte molecule conjugated with the nanoparticle, and a sensor circuit communicatively connected to the biosensor, wherein the sensor circuit is configured to detect at least an analyte characteristic of the analyte.

Abstract: An apparatus for controlling assay steps using active flow components, the apparatus includes a microfluidic device containing a reservoir configured to contain a fluid and microfluidic channels connected to the reservoir, wherein the microfluidic channels are configured to create a microfluidic environment for an assay, active flow components, a sensor device configured to detect a sensed property of the fluid, and an external device connected to the microfluidic device including actuators, wherein the actuators are configured to connect the active flow components using a mechanical interface and initiate active flow processes corresponds to assay steps of the assay based on the active flow components, and a reading device configured to read the sensed property of the fluid from the sensor device, wherein the active flow components are configured to flow the fluid bi-directionally through the sensor device within the microfluidic environment based on active flow processes.

Abstract: Apparatus of a multipurpose fluid extraction device for diagnostics and method of use are disclosed. The apparatus includes a fluid extraction system, wherein the fluid extraction system is configured to extract a fluid from a user, a microfluidic assembly, wherein the microfluidic assembly is configured to provide a flow of extracted fluid, where the microfluidic assembly includes a microfluidic channel, a photonic sensor, wherein the photonic sensor is configured to output a sensor signal to a reader device that is configured to detect one or more characteristics of the extracted fluid as a function of the sensor signal, an assay component fluidically connected to the microfluidic assembly, wherein the assay component is configured for testing the extracted fluid and a fluid collecting reservoir fluidically connected to the microfluidic assembly, wherein the fluid collecting reservoir is configured to collect the extracted fluid from the microfluidic assembly.

Abstract: The invention relates generally to systems and methods for assaying a biological sample suspected of comprising a target biomolecule using cascading amplification.

Abstract: Systems and methods for coupling photonic integrated subcircuits are described herein. The example system can include a first cartridge (4702) including a first photonic integrated subcircuit (4706) and a first alignment feature (4720, 4722). The system can include a second cartridge (4704) including a second photonic integrated subcircuit (4708) and a second alignment feature (4724, 4726), where the first alignment feature (4720, 4722) and the second alignment feature (4724, 4726) can be configured to enable alignment between the first photonic integrated subcircuit (4706) and the second photonic integrated subcircuit (4708). When the first photonic integrated subcircuit (4706) is aligned to the second photonic integrated subcircuit (4708), a first light path of the first photonic integrated subcircuit (4706) can be optically coupled to a second light path of the second photonic integrated subcircuit (4708).

Abstract: Systems and methods for generating a virtual environment for implementing an integrated photonics assembly are presented. An example system can include one or more processors and a memory coupled with the processors, where the processor executes a plurality of modules stored in the memory. The plurality of modules can include a user interface module for deploying one or more virtual photonic integrated subcircuits within the virtual environment, in which the virtual environment is configured to enable coupling of at least two virtual photonic integrated subcircuits. The coupling of the virtual photonic integrated subcircuits can form a virtual integrated photonics assembly. The modules can include a library module comprising a plurality of virtual photonic integrated subcircuits. One or more virtual photonic integrated subcircuits can include a performance characteristic.

Abstract: Disclosed herein are integrated photonics systems (3800) for biosensing including an interrogator photonic circuit (3802) and cartridge (3804) and methods using these systems. The cartridge (3804) comprises a sensor photonic integrated subcircuit. The cartridge (3804) is configured to receive a biological sample. The interrogator photonic circuit (3802) is optically coupled to the cartridge (3804) an comprises: (i) a light source (3806) configured to generate light; and (ii) one or more waveguides configured to carry the light, wherein the light is used to determine a characteristic of the biological sample in the cartridge (3804). A system can have an assembly of a plurality of modular photonic integrated subcircuits. Each subcircuit can be pre-fabricated and can be configured to transfer light to and receive light from another subcircuit based on the first functionality.

Abstract: Aspects relate to systems and methods for fluid sensing using passive flow. An exemplary system includes a microfluidic device, the microfluidic device including at least a reservoir configured to contain at least a fluid and at least a passive flow component in fluidic communication with the at least a reservoir and configured to flow the at least a fluid with predetermined flow properties, at least an sensor device configured to be in sensed communication with the at least a fluid and detect at least a sensed property; and at least an sensor interface configured to wet at least a surface of the at least a sensor device with the at least a fluid.

Abstract: A method of tagged-base DNA sequencing readout on waveguide surfaces includes immobilizing, a surface of a waveguide, a nucleotide fragment, exposing the nucleotide fragment to a first plurality of capped nucleotides, wherein the first plurality of capped nucleotides include a first plurality of nucleotide types, each distinct nucleotide type has a distinct capping agent, and each distinct capping agent has a distinct optical signature, severing base pair connections between the at least a nucleotide fragment and the first plurality of capped nucleotides, wherein the nucleotide fragment remains attached and a first single nucleotide, of the first plurality of capped nucleotides, remains immobilized on a nucleotide binding locus adjacent to the first nucleotide sequence, and detecting a first distinct optical signature of a first distinct capping agent of the first single nucleotide using the waveguide.

Abstract: Aspects relate to systems and methods for high-contrast cleavage detection. An exemplary method includes a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent, a waveguide configured to propagate an electromagnetic (EM) wave, wherein the waveguide includes a surface upon which the plurality of probes are immobilized, wherein the surface is configured to provide communication between the EM wave and the high-contrast agent, a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent and the plurality of probes are configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte, and a sensor in communication with the waveguide and configured to detect cleaving of the at least a probe by way of the EM wave.

Abstract: Aspects relate to methods and systems for optical matrix calculation. An exemplary system includes at least a first light source configured to output at least a first optical output having a first wavelength, at least a second light source configured to output at least a second optical output having a second wavelength substantially different from the first wavelength, at least an optical modulator configured to modulate the at least a first optical output, at least an optical matrix multiplier configured to perform at least two matrix multiplications, a first matrix multiplication as a function of the first optical output and a second matrix multiplication as a function of the second optical output, and at least a photodetector configured to measure the at least a first optical output and the at least a second optical output.

Abstract: The Anti-idling System for Ambulances and Other Vehicles is a novel system for making electricity available to vehicles which idle curbside in urban and suburban environments. The invention comprises a pedestal with a specialized electricity plug, a cord and cord reel, an onboard microcontroller with GSM and RFID card reader which controls the flow of electricity and communicates with a cloud-based management system, and a cloud-based management system which controls user authentication. This enables an ambulance or other vehicle operator to approach the pedestal, access the plug and cord and turn on the flow of electricity using an RFID card or mobile phone, and use the pedestal to power their vehicle while in proximity to the pedestal. When the vehicle engine is started, the system detects the auto-ejection of the plug from the vehicle, turns off the flow of electricity, and automatically retracts the plug and cord into the pedestal.

Abstract: The Anti-idling System for Ambulances and Other Vehicles is a novel system for making electricity available to vehicles which idle curbside in urban and suburban environments. The invention comprises a pedestal with a specialized electricity plug, a cord and cord reel, an onboard microcontroller with GSM and RFID card reader which controls the flow of electricity and communicates with a cloud-based management system, and a cloud-based management system which controls user authentication. This enables an ambulance or other vehicle operator to approach the pedestal, access the plug and cord and turn on the flow of electricity using an RFID card or mobile phone, and use the pedestal to power their vehicle while in proximity to the pedestal. When the vehicle engine is started, the system detects the auto-ejection of the plug from the vehicle, turns off the flow of electricity, and automatically retracts the plug and cord into the pedestal.

Abstract: The Anti-idling System for Ambulances and Other Vehicles is a novel system for making electricity available to vehicles which idle curbside in urban and suburban environments. The invention comprises a pedestal with a specialized electricity plug, a cord and cord reel, an onboard microcontroller with GSM and RFID card reader which controls the flow of electricity and communicates with a cloud-based management system, and a cloud-based management system which controls user authentication. This enables an ambulance or other vehicle operator to approach the pedestal, access the plug and cord and turn on the flow of electricity using an RFID card or mobile phone, and use the pedestal to power their vehicle while in proximity to the pedestal. When the vehicle engine is started, the system detects the auto-ejection of the plug from the vehicle, turns off the flow of electricity, and automatically retracts the plug and cord into the pedestal.

Abstract: A process for treating acid mine drainage containing heavy metals and soluble contaminants is provided. In one embodiment, at least a metal cation is added to the acid mine drainage at a pre-select pH to form insoluble heavy metal complexes. In one embodiment, the metal cation is a trivalent metal ion, e.g., ferric iron such as in ferric sulfate. In another embodiment, a divalent metal ion such as in ferrous sulfate is used. After the removal of the heavy metal complexes, the effluent water is treated with at least a phosphate additive to remove remaining soluble contaminants, thus producing a treated water stream with reduced levels of contaminants.

Abstract: The present invention relates to a chewing gum base formulation comprising a biocompatible particulate polymer having an average particle size of from about 200 ?m to about 2000 ?m wherein the polymer fibrillates when subjected to a shear stress such as that encountered during chewing.

Abstract: A process for treating acid mine drainage containing heavy metals and soluble contaminants is provided. In one embodiment, at least a metal cation is added to the acid mine drainage at a pre-select pH to form insoluble heavy metal complexes. In one embodiment, the metal cation is a trivalent metal ion, e.g., ferric iron such as in ferric sulfate. In another embodiment, a divalent metal ion such as in ferrous sulfate is used. After the removal of the heavy metal complexes, the effluent water is treated with at least a phosphate additive to remove remaining soluble contaminants, thus producing a treated water stream with reduced levels of contaminants.