Abstract: Provided herein are fusion proteins and methods of use thereof to purify nucleic acids from a composition. The disclosed compositions and methods allow for faster, more efficient purification of nucleic acids compared to traditional affinity chromatography.

Abstract: Provided herein are fusion proteins comprising a first polypeptide and a second polypeptide, wherein the second polypeptide has phase behavior. The first polypeptide may be a polypeptide having a desirable biological activity, such as a polypeptide with therapeutic, cosmetic, and/or industrial importance. Fusing the first polypeptide to the polypeptide with phase behavior facilitates purification thereof, and may also help to stabilize the first polypeptide during expression, storage, or after exposure to various conditions known to unfold, degrade, or misfold proteins.

Abstract: Disclosed herein are fusion proteins comprising the CR3 domain of the low density lipoprotein (LDLR) and a polypeptide with phase behavior. Also provided herein are polypeptides with phase behavior. Additionally provided herein are methods of improving or decreasing viral transduction efficiency comprising administering a fusion protein or polypeptide with phase behavior described herein.

Abstract: Disclosed herein are purification matrices comprising adeno-associated virus (AAV)-binding polypeptides and methods of using the same.

Abstract: Provided herein are protein-based purification matrices and methods of use thereof to purify biologics and/or to remove contaminants from a composition. Methods of bringing two or more biologics in close proximity are also provided. The disclosed compositions and methods allow for faster, more efficient purification of a biologic compared to traditional affinity chromatography.

Abstract: Provided herein are protein-based purification matrices and methods of use thereof to purify biologics and/or to remove contaminants from a composition. Methods of bringing two or more biologics in close proximity are also provided. The disclosed compositions and methods allow for faster, more efficient purification of a biologic compared to traditional affinity chromatography.