Abstract: Disclosed herein are processes for ethanol production from a lignocellulosic feedstock. These processes provide DMR of lignocellulosic biomass comprising two-stage deacetylation followed by mechanical refining so as to increase fermentable sugar yield while reducing hydrolytic enzyme loading requirements.

Abstract: Disclosed herein are processes for ethanol production from a lignocellulosic feedstock. These processes provide DMR of lignocellulosic biomass comprising two-stage deacetylation followed by mechanical refining so as to increase fermentable sugar yield while reducing hydrolytic enzyme loading requirements.

Abstract: The utilization of the byproducts from the deacetylation of lignocellulosic biomass from the production of ethanol to create cement additives and/or alternatives to ordinary portland cement (OPC) is described. These byproducts may be used to create black liquor bioash (BLA), activated lignin carbon (ALC), and/or lignin aerogel admixtures. In some embodiments, the byproduct may be combined with at least one binder to form alkali-activated materials (AAMs).

Abstract: Disclosed herein are a cellulose particle and a binder, where the cellulose particle has a cellulose core and a surface group, and the binder interacts with the surface group to form a composite.

Abstract: A range of Cel7 putative cellobiohydrolase genes were identified using genome mining and homologous sequence alignment to Cel7A from Trichoderma reesei. A representative subset of these genes from across a broad diversity of evolutionarily disparate sources were cloned and expressed in T. reesei using a constitutive promotor and a common secretion signal. The purified recombinant enzymes were tested for efficacy on various substrates. The top performers were subjected to structural studies and subsites likely to confer enhanced performance were predicted using homology modeling and comparisons of natural sequence diversity. Once identified, the subsites were genetically introduced individually and combinatorically into the best in class Cel7A backbone we have found to date and then expressed in T. reesei and tested. A triple mutant was determined to have the highest cellulase activity we have measured in a cellobiohydrolase to date.

Abstract: Disclosed herein are processes for ethanol production from a lignocellulosic feedstock. These processes provide DMR of lignocellulosic biomass comprising two-stage deacetylation followed by mechanical refining so as to increase fermentable sugar yield while reducing hydrolytic enzyme loading requirements.

Abstract: Disclosed herein is a novel, cofactor balanced, cell-free biocatalysis pathway to make diacids from formaldehyde or methanol that does not use ATP and produces no CO2. In another embodiment, disclosed herein is a novel C5/C6 (hydrolysate) utilization pathway that interplays with the cell making diacids from formaldehyde or methanol that does not use ATP, produces no CO2, and replaces ATP with (cheap) polyphosphate (no other cofactors needed). Using methods and compositions disclosed herein, no CO2 is produced and the pathways disclosed herein are cell-free. The combination of pathways, feedstocks and products disclosed herein is novel.

Abstract: The present disclosure relates to a composition that includes a base layer having an outer surface and a first thickness and a material that includes a plurality of cellulose nanofibers in physical contact with the outer surface, where the composition has an average filtration efficiency of less than or equal to 90% for particles having a characteristic length between about 50 nm and about 100 ?m and the composition is characterized by an average inhalation resistance of less than or equal to 35 mm H2O as measured across the first thickness and the material.

Abstract: Non-naturally occurring Zymomonas strains useful for the production of 2,3-butanediol are provided.

Abstract: Disclosed herein are a cellulose particle and a binder, where the cellulose particle has a cellulose core and a surface group, and the binder interacts with the surface group to form a composite.

Abstract: Disclosed herein are a cellulose particle and a binder, where the cellulose particle has a cellulose core and a surface group, and the binder interacts with the surface group to form a composite.

Abstract: Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.

Abstract: Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases.

Abstract: Disclosed herein are chimeric Cel7A polypeptides useful for producing biofuels from lignocellulosic biomass.

Abstract: Non-naturally occurring Zymomonas strains useful for the production of 2,3-butanediol are provided.

Abstract: Disclosed herein are chimeric Cel7A polypeptides useful for producing biofuels from lignocellulosic biomass.

Abstract: Disclosed herein are a cellulose particle and a binder, where the cellulose particle has a cellulose core and a surface group, and the binder interacts with the surface group to form a composite.

Abstract: Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases.

Abstract: Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

Abstract: Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.