Abstract: Disclosed herein include methods, compositions, and kits suitable for use in the measurement of the states of living cells across time and for use in the delivery of polyribonucleotides and circuits. There are provided, in some embodiments, RNA exporter proteins comprising an RNA-binding domain, a membrane-binding domain, and an interaction domain capable of nucleating self-assembly. Disclosed herein include polynucleotides encoding reporter RNA molecule(s) or cargo RNA molecule(s). In some embodiments, a plurality of RNA exporter proteins are capable of self-assembling into lipid-enveloped nanoparticles (LNs) secreted from a cell in which the RNA exporter proteins are expressed, thereby generating a population of LNs comprising exported reporter RNA molecule(s) or a fusogen and exported cargo RNA molecule(s). Disclosed herein include export modulator proteins capable of, e.g., enhancing or suppressing LN export.

Abstract: In a preferred embodiment, a fluid-operated switch continuously and selectively adjustable over both a pressure range and a vacuum range. The switch includes a diaphragm enclosed in a housing, a first side of the diaphragm communicating with the fluid. On the second side of the diaphragm, actuator means are provided which actuate an electrical switch when the diaphragm is flexed by changes in the pressure or vacuum of the fluid. A main spring provides adjustable resistance to motion of the actuator means in the direction of diaphragm flexure corresponding to an increase in pressure, while a counterspring provides resistance to motion of the actuator means in the direction of diaphragm flexure corresponding to an increase in vacuum. Adjustment of the set point of the switch is effected through adjusting the degree of compression or relaxation of the springs by adjusting a threaded bonnet on the housing.