Patents by Inventor Michael G. Bramucci

Michael G. Bramucci has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Butanol dehydrogenase enzyme from the bacterium
    Patent number: 9447385
    Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
    Type: Grant
    Filed: February 13, 2014
    Date of Patent: September 20, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
  • YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING CELL WALL PROTEINS
    Publication number: 20160138050
    Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. The yeast host cells provided herein comprise at least one genetic modification in a pyruvate decarboxylase gene and at least one genetic modification in an endogenous cell wall protein, which confers resistance to butanol and increased glucose utilization.
    Type: Application
    Filed: July 14, 2014
    Publication date: May 19, 2016
    Applicant: Butamax Advanced Biofuels LLC
    Inventor: Michael G. BRAMUCCI
  • Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
    Patent number: 9284612
    Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: March 15, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Der-Ing Liao, Mark J. Nelson, Michael G. Bramucci
  • Butanol tolerance in microorganisms
    Patent number: 9273330
    Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: March 1, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Vasantha Nagarajan
  • Yeast with increased butanol tolerance involving cell wall integrity pathway
    Patent number: 8795992
    Abstract: Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the cell wall integrity pathway. Yeast with increased expression of SLT2p, a mitogen activated protein kinase of the MAPK module of the cell wall integrity pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.
    Type: Grant
    Filed: December 21, 2009
    Date of Patent: August 5, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Robert A. Larossa, Dana R. Smulski
  • Butanol Dehydrogenase Enzyme from the Bacterium Achromobacter xylosoxidans
    Publication number: 20140170732
    Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
    Type: Application
    Filed: February 13, 2014
    Publication date: June 19, 2014
    Applicant: BUTAMAX ADVANCED BIOFUELS LLC
    Inventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
  • BUTANOL TOLERANCE IN MICROORGANISMS
    Publication number: 20140096439
    Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.
    Type: Application
    Filed: October 3, 2013
    Publication date: April 10, 2014
    Inventors: MICHAEL G. BRAMUCCI, VASANTHA NAGARAJAN
  • Butanol dehydrogenase enzyme from the bacterium
    Patent number: 8691540
    Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
    Type: Grant
    Filed: May 24, 2012
    Date of Patent: April 8, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
  • Yeast with increased butanol tolerance involving filamentous growth response
    Patent number: 8557562
    Abstract: Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the filamentous growth response. Yeast with increased expression of MSS11p, a transcriptional activator of the filamentous growth response pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.
    Type: Grant
    Filed: December 21, 2009
    Date of Patent: October 15, 2013
    Assignee: Butamax(TM) Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Robert A. Larossa, Manjari Singh
  • Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
    Patent number: 8455225
    Abstract: Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production.
    Type: Grant
    Filed: December 21, 2009
    Date of Patent: June 4, 2013
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Robert A. Larossa, Dana R. Smulski
  • Method for the production of 1-butanol
    Patent number: 8426173
    Abstract: A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.
    Type: Grant
    Filed: April 28, 2008
    Date of Patent: April 23, 2013
    Assignee: Butamax (TM) Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Dennis Flint, Edward S. Miller, Jr., Vasantha Nagarajan, Natalia Sedkova, Manjari Singh, Tina K. Van Dyk
  • Method for the production of 2-butanol
    Patent number: 8426174
    Abstract: A method for the production of 2-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.
    Type: Grant
    Filed: April 28, 2008
    Date of Patent: April 23, 2013
    Assignee: Butamax(TM) Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Dennis Flint, Edward S. Miller, Jr., Vasantha Nagarajan, Natalia Sedkova, Manjari Singh, Tina K. Van Dyk
  • BUTANOL DEHYDROGENASE ENZYME FROM THE BACTERIUM ACHROMOBACTER XYLOSOXIDANS
    Publication number: 20120231515
    Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
    Type: Application
    Filed: May 24, 2012
    Publication date: September 13, 2012
    Applicant: Butamax(TM) Advanced Biofuels LLC
    Inventors: MICHAEL G. BRAMUCCI, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
  • YEAST PRODUCTION CULTURE FOR THE PRODUCTION OF BUTANOL
    Publication number: 20120149080
    Abstract: High cell density cultures of yeast were found to have higher tolerance for butanol in the medium. The high cell density yeast cultures had greater survival and higher glucose utilization than cultures with low cell densities. Production of butanol using yeast in high cell density cultures is thus beneficial for improving butanol production.
    Type: Application
    Filed: June 16, 2011
    Publication date: June 14, 2012
    Applicant: Butamax(TM) Advanced Biofuels LLC
    Inventors: VASANTHA NAGARAJAN, Michael G. Bramucci
  • Butanol dehydrogenase enzyme from the bacterium
    Patent number: 8188250
    Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
    Type: Grant
    Filed: April 27, 2009
    Date of Patent: May 29, 2012
    Assignee: Butamax(TM) Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
  • FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY ACTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES
    Publication number: 20110250610
    Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
    Type: Application
    Filed: February 22, 2011
    Publication date: October 13, 2011
    Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLC
    Inventors: DER-ING LIAO, MARK J. NELSON, MICHAEL G. BRAMUCCI
  • Solvent tolerant microorganisms
    Patent number: 8017364
    Abstract: Pediococcus bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentative production of butanol.
    Type: Grant
    Filed: December 4, 2007
    Date of Patent: September 13, 2011
    Inventors: Michael G. Bramucci, Helene M. A. Kane, Vasantha Nagarajan
  • Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
    Patent number: 7910342
    Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
    Type: Grant
    Filed: April 16, 2008
    Date of Patent: March 22, 2011
    Assignee: Butamax(TM) Advanced Biofuels LLC
    Inventors: Der-Ing Liao, Mark J. Nelson, Michael G. Bramucci
  • YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING CELL WALL INTEGRITY PATHWAY
    Publication number: 20100167364
    Abstract: Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the cell wall integrity pathway. Yeast with increased expression of SLT2p, a mitogen activated protein kinase of the MAPK module of the cell wall integrity pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.
    Type: Application
    Filed: December 21, 2009
    Publication date: July 1, 2010
    Applicant: Butamax (TX) Advanced Biofuels LLC
    Inventors: MICHAEL G. BRAMUCCI, ROBERT A. LAROSSA, DANA R. SMULSKI
  • YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING HIGH OSMOLARITY/GLYCEROL RESPONSE PATHWAY
    Publication number: 20100167365
    Abstract: Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production.
    Type: Application
    Filed: December 21, 2009
    Publication date: July 1, 2010
    Applicant: Butamax (TM) Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Robert A. Larossa, Dana R. Smulski