Abstract: Devices that may couple two or more apparatuses, such as an organ-on-a-chip device and a microfluidic device. Devices that include an organ-on-a-chip device, a microfluidic device, and a cap that couples the organ-on-a-chip device and the microfluidic device. Systems that include the devices and a detection unit. Methods for quantitation of insulin.

Abstract: Methods and systems for insulin detection. The methods may include contacting one or more islets with glucose to produce a first stream that is then contacted with an anti-insulin antibody and a labeled insulin to produce a second stream. The second stream may be analyzed to determine a ratio of antibody-bound (B) labeled insulin to free (F) labeled insulin in the second stream, wherein the ratio of B:F is inversely related to a concentration of target insulin.

Abstract: Devices that may couple two or more apparatuses, such as an organ-on-a-chip device and a microfluidic device. Devices that include an organ-on-a-chip device, a microfluidic device, and a cap that couples the organ-on-a-chip device and the microfluidic device. Systems that include the devices and a detection unit. Methods for quantitation of insulin.

Abstract: A sample preparation device for electron microscopy (EM) that is configured to eliminate user-to-user variations and environment contaminations, which are often present in the conventional method of sample preparation. The device not only provides a means for evenly and reproducibly delivering a fluid or sample to an EM grid, but also provides a means for sealing the EM grid in an air-tight chamber and delivering air-sensitive samples to the EM grid. The platform may comprise readily fabricated glass chips with features integrated to preserve the integrity of the sample grid and to facilitate its extraction. The methods may eliminate the element of user dependent variability and thus improve the throughput, reproducibility and translation of these methods.

Abstract: Provided herein are methods for multiplexed sample analysis by mass spectrometry. The methods may be performed without the need for chemical tagging. The methods also may include the analogous use of frequency modulation to multiplex mass spectrometric analysis, which may be referred to as frequency-modulated continuous flow analysis electrospray ionization mass spectrometry (FM-CFA-ESI-MS).

Abstract: Provided herein are methods for multiplexed sample analysis by mass spectrometry. The methods may be performed without the need for chemical tagging. The methods also may include the analogous use of frequency modulation to multiplex mass spectrometric analysis, which may be referred to as frequency-modulated continuous flow analysis electrospray ionization mass spectrometry (FM-CFA-ESI-MS).

Abstract: A sample preparation device for electron microscopy (EM) that is configured to eliminate user-to-user variations and environment contaminations, which are often present in the conventional method of sample preparation. The device not only provides a means for evenly and reproducibly delivering a fluid or sample to an EM grid, but also provides a means for sealing the EM grid in an air-tight chamber and delivering air-sensitive samples to the EM grid. The platform may comprise readily fabricated glass chips with features integrated to preserve the integrity of the sample grid and to facilitate its extraction. The methods may eliminate the element of user dependent variability and thus improve the throughput, reproducibility and translation of these methods.

Abstract: A sample preparation device for electron microscopy (EM) that is configured to eliminate user-to-user variations and environment contaminations, which are often present in the conventional method of sample preparation. The device not only provides a means for evenly and reproducibly delivering a fluid or sample to an EM grid, but also provides a means for sealing the EM grid in an air-tight chamber and delivering air-sensitive samples to the EM grid. The platform may comprise readily fabricated glass chips with features integrated to preserve the integrity of the sample grid and to facilitate its extraction. The methods may eliminate the element of user dependent variability and thus improve the throughput, reproducibility and translation of these methods.

Abstract: An apparatus and associated method for attaching a disc drive cover member to a disc drive base member. A fixture comprises a base and a stop guard, cradle and guide rails supported by the base. The disc drive base member is operatively receivingly engaged in the cradle adjacent the guide rails. The disc drive cover member is operatively abuttingly engaged at one end against the stop guard and pivoted toward the disc drive base member. Any substantial misalignment of the disc drive cover member with the disc drive base member causes the disc drive cover member to engage at least one of the guide rails and therefore be urged into proper alignment, preventing adverse contact between the disc drive cover member and the disc drive base member.